Articles producció científica> Bioquímica i Biotecnologia

Role of Mitochondrial Retrograde Pathway in Regulating Ethanol-Inducible Filamentous Growth in Yeast

  • Identification data

    Identifier: PC:2804
    Authors:
    Mas, A.González, B.Beltran, G.Cullen, P.J.Torija, M.J.
    Abstract:
    In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.
  • Others:

    Author, as appears in the article.: Mas, A.; González, B.; Beltran, G.; Cullen, P.J.; Torija, M.J.
    Department: Bioquímica i Biotecnologia
    URV's Author/s: MAS BARON, ALBERTO; González, B.; BELTRAN CASELLAS, GEMMA; Cullen, P.J.; TORIJA MARTÍNEZ, MARÍA JESÚS
    Keywords: filamentous growth pseudohyphal growth quorum sensing
    Abstract: In yeast, ethanol is produced as a by-product of fermentation through glycolysis. Ethanol also stimulates a developmental foraging response called filamentous growth and is thought to act as a quorum-sensing molecule. Ethanol-inducible filamentous growth was examined in a small collection of wine/European strains, which validated ethanol as an inducer of filamentous growth. Wine strains also showed variability in their filamentation responses, which illustrates the striking phenotypic differences that can occur among individuals. Ethanol-inducible filamentous growth in 61278b strains was independent of several of the major filamentation regulatory pathways [including fMAPK, RAS-cAMP, Snf1, Rpd3(L), and Rim101] but required the mitochondrial retrograde (RTG) pathway, an inter-organellar signaling pathway that controls the nuclear response to defects in mitochondrial function. The RTG pathway regulated ethanol-dependent filamentous growth by maintaining flux through the TCA cycle. The ethanol-dependent invasive growth response required the polarisome and transcriptional induction of the cell adhesion molecule Flo11p. Our results validate established stimuli that trigger filamentous growth and show how stimuli can trigger highly specific responses among individuals. Our results also connect an inter-organellar pathway to a quorum sensing response in fungi.
    Research group: Biotecnologia Enològica
    Thematic Areas: Enologia Enología Oenology
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664-042X
    Author identifier: 0000-0002-0763-1679; n/a; 0000-0002-7071-205X; n/a; 0000-0001-6419-0745
    Record's date: 2017-05-18
    Journal volume: 8
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: http://journal.frontiersin.org/article/10.3389/fphys.2017.00148/full
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.3389/fphys.2017.00148
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    First page: Art.num. 148
    Publication Type: Article Artículo Article
  • Keywords:

    Llevats
    Mitocondris
    filamentous growth
    pseudohyphal growth
    quorum sensing
    Enologia
    Enología
    Oenology
    1664-042X
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