Author, as appears in the article.: Figueras, M.J.; Talagrand-Reboul, E.; Roger, F.; Kimper, J.-L.; Colston, S.M.; Graf, J.; Latif-Eugenín, F.; Petit, F.; Marchandin, H.; Jumas-Bilak, E.; Lamy, B.
Department: Ciències Mèdiques Bàsiques
URV's Author/s: FIGUERAS SALVAT, MARIA JOSEFA; Talagrand-Reboul, E.; Roger, F.; Kimper, J.-L.; Colston, S.M.; Graf, J.; Latif-Eugenín, F.; Petit, F.; Marchandin, H.; Jumas-Bilak, E.; Lamy, B.
Keywords: Integrative taxonomy Complex of species; Aeromonas
Abstract: Aeromonas media is an opportunistic pathogen for human and animals mainly found in aquatic habitats and which has been noted for significant genomic and phenotypic heterogeneities. We aimed to better understand the population structure and diversity of strains currently affiliated to A. media and the related species A. rivipollensis. Forty-one strains were included in a population study integrating, multilocus genetics, phylogenetics, comparative genomics, as well as phenotypics, lifestyle, and evolutionary features. Sixteen gene-based multilocus phylogeny delineated three clades. Clades corresponded to different genomic groups or genomospecies defined by phylogenomic metrics ANI (average nucleotide identity) and isDDH (in silico DNA-DNA hybridization) on 14 whole genome sequences. DL-lactate utilization, cefoxitin susceptibility, nucleotide signatures, ribosomal multi-operon diversity, and differences in relative effect of recombination and mutation (i.e., in evolution mode) distinguished the two species Aeromonas media and Aeromonas rivipollensis. The description of these two species was emended accordingly. The genome metrics and comparative genomics suggested that a third clade is a distinct genomospecies. Beside the species delineation, genetic and genomic data analysis provided a more comprehensive knowledge of the cladogenesis determinants at the root and inside A. media species complex among aeromonads. Particular lifestyles and phenotypes as well as major differences in evolution modes may represent putative factors associated with lineage emergence and speciation within the A. media complex. Finally, the integrative and populational approach presented in this study is considered broadly in order to conciliate the delineation of taxonomic species and the population structure in bacterial genera organized in species complexes.
Research group: Unitat de Micologia i Microbiologia Ambiental
Thematic Areas: Health sciences Ciencias de la salud Ciències de la salut
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1664-302X
Author identifier: 0000-0002-2268-8980; ; ; ; ; ; ; ; ; ;
Record's date: 2017-05-26
Journal volume: 8
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.frontiersin.org/articles/10.3389/fmicb.2017.00621
Funding program: plan; JPIW2013-095-CO3 plan; AGL2011- 30461-C02-02
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3389/fmicb.2017.00621
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
First page: 621
Publication Type: Article Artículo Article
Repositori URV