Author, as appears in the article.: Fabiola Fernández-Silva Michaela Lackner Javier Capilla Emilio Mayayo Deanna Sutton Mariana Castanheira Annette W Fothergill
Department: Ciències Mèdiques Bàsiques
e-ISSN: 1098-6596
URV's Author/s: Fernández Silva, Fabiola Vanessa Michaela Lackner Capilla Luque, Javier Mayayo Artal , Emilio Manuel Deanna Sutton Mariana Castanheira Annette W Fothergill Cornelia Lass-Flörl Guarro Artigas , Josep
Keywords: Candida glabrata Candida glabrata Candida glabrata
Abstract: It has been argued that in vitro activity of caspofungin (CSP) is not a good predictor
of the in vivo outcome of echinocandins treatment. We evaluated the in vitro activity of CSP and the presence of FKS mutations in the hot spot 1 (HS1) region of FKS1 and FKS2 genes of 17 Candida glabrata strains with a wide MICs range. The efficacy of CSP against systemic infections by all those strains was evaluated in a murine model. No HS1 mutations were found in the eight strains showing MICs of CSP ≤ 0.5 μg/ml, but they were present in eight of the nine strains with MICs ≥ 1 μg/ml, i.e. three in the FKS1 and five in the FKS2 genes. CSP was effective to treat mice infected with strains with MICs ≤ 0.5μg/ml, showed variable efficacy in animals challenged with strains with MICs = 1μg/ml and did not work in those with strains with MICs > 1μg/ml. In addition, mutations outside the HS1 region were found in the FKS 2 gene of six strains with different MICs, including a first time reported mutation, but their presence did not influence the drug efficacy. In vitro activity of CSP was compared with other echinocandin i.e., anidulafungin suggesting that MICs of both drugs as well as mutations in the HS1 regions of FKS1 or FKS2 genes are predictive of the outcome.
Research group: Unitat de Micologia i Microbiologia Ambiental
licence for use: thttps://creativecommons.org/licenses/by/3.0/es/
ISSN: 0066-4804
Author identifier: N/A N/A N/A N/A N/A N/A N/A N/A N/A
Record's date: 2014-05-14
Last page: 3649
Journal volume: 58
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://aac.asm.org/content/58/7/3646.long
Article's DOI: 10.1128/AAC.02666-13
Entity: Universitat Rovira i Virgili
Journal publication year: 2014
First page: 3646
Publication Type: Article Artículo Article
Repositori URV