Articles producció científica> Química Analítica i Química Orgànica

Performance of zwitterionic hydrophilic interaction LC for the determination of iodinated X-ray contrast agents

  • Identification data

    Identifier: PC:594
    Handle: http://hdl.handle.net/20.500.11797/PC594

  • Authors:

    Pocurull, E.
    Fontanals, N.
    Borrull, F.
    Herrero, P.
    Echeverría, S.

  • Others:

    Author, as appears in the article.: Pocurull, E. Fontanals, N. Borrull, F. Herrero, P. Echeverría, S.
    Department: Química Analítica i Química Orgànica
    e-ISSN: 1615-9314
    Abstract: This study compares the separation performance of a group of iodinated X-ray contrast media on four different columns. The first three were two stationary phases (SPs) modified with C18 and a polar-embedded SP (polar amide group bonded to an alkyl chain), all of which worked under RP-LC mode. The fourth was a zwitterionic sulphoalkylbetaine SP, working under the hydrophilic interaction LC (HILIC) mode. After the optimisation of the different parameters, the zwitterionic column displayed the best separation, which also overcomes the problems encountered when these analytes were separated under RP-LC. Moreover, when HILIC is coupled to MS/MS, sensitivity is enhanced. However, when sewage samples were analysed by SPE followed by the optimal HILIC-MS/MS, the sensitivity of the method was affected due to the high matrix effect, which had to be solved by dilution of the extract. Finally, the method was preliminarily validated with sewage and the figures of merit were comparable to those of the SPE-RP-LC-MS/MS.
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1615-9306
    Last page: 3695
    Journal volume: 36
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/abs/10.1002/jssc.201300702
    Article's DOI: 10.1002/jssc.201300702
    Entity: Universitat Rovira i Virgili.
    Journal publication year: 2013
    First page: 3688