Author, as appears in the article.: Ollendorff, V. Cabello, G. Federici, C. Perroy, J. Mulero, M.
Papper version: info:eu-repo/semantics/publishedVersion
Department: Bioquímica i Biotecnologia
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
URV's Author/s: Miquel Mulero, Julie Perroy, Carole Federici, Gérard Cabello
Abstract: Background Nuclear receptors (NR) regulate transcription of genes involved in many biological processes such as development, cell proliferation, differentiation and cell death. Amongst them, PPARG2 and THR control tissue glucose and lipid homeostasis which are deregulated in severe pathophysiological conditions such as metabolic syndromes. Methodology/Principal Findings Here, we describe a real time BRET approach to monitor heterodimerization between RXR and PPARG2 or THR in vitro or in living cells. The presence of a specific DNA target was required to induce in vitro a BRET shift reflecting heterodimerization of RXR/PPARG2 or RXR/THR. As in electrophoretic mobility shift assay (EMSA), the stringency and specificity of the BRET shift assay depended upon assay condition optimization including MgCl2 concentration. For the nuclear receptors, we found by mutagenesis analysis that each heterodimer partner must harbor an intact DNA binding domain to induce BRET and heterodimerization on a DNA target. Moreover the interaction between the PPARG2 ligand binding domain and the RXR DNA binding domain stabilized the heterodimer on its DNA target. BRET microscopy in living cells highlighted the heterodimerization of RXR/PPARG2 within the nucleus clustered in discrete foci that may represent active target gene transcription regulation regions. BRET imaging also suggested that heterodimerization between RXR and PPARG2 required the DNA binding of PPARG2. Conclusions/Significance The BRET approach described here allowed us to study the dynamic interactions which exist between NR in vitro or in living cells and can provide important information on heterodimerization modes, affinity with a given RE and subcellular localization of the heterodimers. This method could be used to study real time changes of NR heterodimers occurring on DNA depending upon cell activation, chromatin state and help to define the mechanisms of ligands or drug action designed to target NRs.
Entity: Universitat Rovira i Virgili.
Journal publication year: 2013
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1932-6203
Journal volume: 8
Repositori URV