Author, as appears in the article.: Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, Escoté X, Gómez-Serrano M, Peral B, Salvadó L, Fernández-Veledo S, Casals N, Vázquez-Carrera M, Villarroya F, Vendrell JJ, Serra D, Herrero L.
Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques
URV's Author/s: Fernandez Veledo, Sonia / Vendrell Ortega, Juan José
Keywords: Type 2 diabetes Obesity Macrophages Inflammation Fatty acid oxidation Cpt1 Adipocytes obesity macrophages inflammation fatty acid oxidation cpt1 adipocytes
Abstract: Lipid overload in obesity and type 2 diabetes is associated with adipocyte dysfunction, inflammation, macrophage infiltration, and decreased fatty acid oxidation (FAO). Here, we report that the expression of carnitine palmitoyltransferase 1A (CPT1A), the rate-limiting enzyme in mitochondrial FAO, is higher in human adipose tissue macrophages than in adipocytes and that it is differentially expressed in visceral vs. subcutaneous adipose tissue in both an obese and a type 2 diabetes cohort. These observations led us to further investigate the potential role of CPT1A in adipocytes and macrophages. We expressed CPT1AM, a permanently active mutant form of CPT1A, in 3T3-L1 CARΔ1 adipocytes and RAW 264.7 macrophages through adenoviral infection. Enhanced FAO in palmitate-incubated adipocytes and macrophages reduced triglyceride content and inflammation, improved insulin sensitivity in adipocytes, and reduced endoplasmic reticulum stress and ROS damage in macrophages. We conclude that increasing FAO in adipocytes and macrophages improves palmitate-induced derangements. This indicates that enhancing FAO in metabolically relevant cells such as adipocytes and macrophages may be a promising strategy for the treatment of chronic inflammatory pathologies such as obesity and type 2 diabetes.Copyright © 2015 the American Physiological Society.
Thematic Areas: Physiology (medical) Physiology Odontología Nutrição Medicine (all) Medicina ii Medicina i General medicine Farmacia Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas ii Ciências biológicas i Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 15221555
Author's mail: sonia.fernandez@urv.cat jvortega@iispv.cat
Author identifier: 0000-0003-2906-3788 0000-0002-6994-6115
Record's date: 2024-11-09
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://journals.physiology.org/doi/full/10.1152/ajpendo.00362.2014
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: American Journal Of Physiology-Endocrinology And Metabolism. 308 (9): E756-E769
APA: Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, Escoté X, Gómez-Serrano M, Peral B, Salvadó L, Fernández-Veledo S, Casals (2015). Enhanced fatty acid oxidation in adipocytes and macrophages reduces lipid-induced triglyceride accumulation and inflammation. American Journal Of Physiology-Endocrinology And Metabolism, 308(9), E756-E769. DOI: 10.1152/ajpendo.00362.2014
Article's DOI: 10.1152/ajpendo.00362.2014
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
Publication Type: Journal Publications