Articles producció científica> Enginyeria Química

Cell-based assay coupled with chromatographic fractioning: A strategy for marine toxins detection in natural samples

  • Identification data

    Identifier: imarina:3190404
    Authors:
    Diogene, J.Gimenez, G.de la Iglesia, P.Canete, E.Caillaud, A.
    Abstract:
    Cell-based assays (CBA) have been proposed for the evaluation of toxicity caused by marine toxins in natural samples (fish, shellfish and microalgae). However, their application has been hindered due to the interferences present in biological matrices that may cause cellular response and interfere in toxicity evaluation. This work reviews in an extensive introduction the use of CBA for toxicity evaluation of marine toxins. Afterwards, the coupling of chromatographic fractioning with neuroblastoma Neuro-2a CBA is presented to enhance the applicability of CBA for complex matrices. Examples of application are provided for mussel samples (Mytilus galloprovincialis) and microalgae (Gambierdiscus sp.), and the results demonstrated the great potential of the combined strategy for reliable toxicological evaluation without ethical concern. Fractioning of an equivalent of 72 mg eq mL(-1) of mussel sample allowed the identification of non-toxic and toxic fractions whereas only 2.5mg eq mL(-1) of non-purified mussel sample was responsible for 20% of cell mortality. Furthermore, the application of CBA allowed selectively distinguishing between ciguatoxin-like and other unspecific toxicity in Gambierdiscus sp. extract.
  • Others:

    Author, as appears in the article.: Diogene, J. Gimenez, G. de la Iglesia, P. Canete, E. Caillaud, A.
    Department: REVISAR - Enginyeria Química
    URV's Author/s: GIMÉNEZ PAPIOL, GEMMA
    Keywords: SHELLFISH Neuroblastoma Microalgae Marine toxins Gambierdiscus Fractioning Fish Cytotoxicity Cell-Based Assay
    Abstract: Cell-based assays (CBA) have been proposed for the evaluation of toxicity caused by marine toxins in natural samples (fish, shellfish and microalgae). However, their application has been hindered due to the interferences present in biological matrices that may cause cellular response and interfere in toxicity evaluation. This work reviews in an extensive introduction the use of CBA for toxicity evaluation of marine toxins. Afterwards, the coupling of chromatographic fractioning with neuroblastoma Neuro-2a CBA is presented to enhance the applicability of CBA for complex matrices. Examples of application are provided for mussel samples (Mytilus galloprovincialis) and microalgae (Gambierdiscus sp.), and the results demonstrated the great potential of the combined strategy for reliable toxicological evaluation without ethical concern. Fractioning of an equivalent of 72 mg eq mL(-1) of mussel sample allowed the identification of non-toxic and toxic fractions whereas only 2.5mg eq mL(-1) of non-purified mussel sample was responsible for 20% of cell mortality. Furthermore, the application of CBA allowed selectively distinguishing between ciguatoxin-like and other unspecific toxicity in Gambierdiscus sp. extract.
    Thematic Areas: Toxicology Medicine (Miscellaneous)
    Author's mail: gemma.gimenez@urv.cat
    ISSN: 08872333
    Record's date: 2020-01-29
    Papper original source: TOXICOLOGY IN VITRO. (ISSN/ISBN: 08872333). 23(8): 1591-1596
    APA: Caillaud, A.; Canete, E.; de la Iglesia, P.; Gimenez, G.; Diogene, J. (2009). Cell-based assay coupled with chromatographic fractioning: A strategy for marine toxins detection in natural samples. TOXICOLOGY IN VITRO, 23(8), 1591-1596. DOI: 10.1016/j.tiv.2009.08.013
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.tiv.2009.08.013
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2009
    Publication Type: Journal Publications
  • Keywords:

    Medicine (Miscellaneous),Toxicology,Transplantation
    SHELLFISH
    Neuroblastoma
    Microalgae
    Marine toxins
    Gambierdiscus
    Fractioning
    Fish
    Cytotoxicity
    Cell-Based Assay
    Toxicology
    Medicine (Miscellaneous)
    08872333
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