Author, as appears in the article.: Tomas-Hernández S, Blanco J, Rojas C, Roca-Martínez J, Ojeda-Montes M, Beltrán-Debón R, Garcia-Vallvé S, Pujadas G, Arola L, Mulero M
Department: Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
URV's Author/s: Arola Ferrer, Luis Maria / Beltrán Debón, Raúl Alejandro / Blanco Pérez, Jordi / Garcia Vallve, Santiago / Mulero Abellán, Miguel / OJEDA MONTES, Mª JOSÉ / Pujadas Anguiano, Gerard / ROJAS DOMÍNGUEZ, CRISTINA / TOMAS HERNÁNDEZ, SARA
Keywords: Resveratrol Quercetin Protective role Pathway P70s6 kinase Molecular-mechanisms Lysosomal membrane permeabilization Ischemia/reperfusion injury Inhibition In-vitro Human leukemia-cells Hepatic steatosis Heme oxygenase 1 Ellagic acid Death Autophagy Apoptosis Amp-activated protein kinase quercetin p70s6 kinase lysosomal membrane permeabilization heme oxygenase 1 autophagy apoptosis amp-activated protein kinase
Abstract: Resveratrol (RSV) has been described as a potent antioxidant, anti-steatotic, and antitumor compound, and it has also been identified as a potent autophagy inducer. On the other hand, quercetin (QCT) is a dietary flavonoid with known antitumor, anti-inflammatory and antidiabetic effects. Additionally, QCT increases autophagy. To study the hypothetical synergistic effect of both compounds, we tested the combined effect of QCT and RSV on the autophagy process in HepG2 cells METHODS AND RESULTS: Autophagy was studied by western blotting, real-time RT-PCR, and cellular staining. Our results clearly indicate a bifunctional molecular effect of RSV. Both polyphenols were individually able to promote autophagy. Strikingly, when RSV was combined with QCT, it promoted a potent reduction of QCT-induced autophagy and influenced pro-apoptotic signaling. Conclusion: RSV acts differentially on the autophagic process depending on the cellular energetic state. We further characterized the molecular mechanisms related to this effect, and we observed that AMP-activated protein kinase (AMPK) phosphorylation, heme oxygenase 1 (HO-1) downregulation, lysosomal membrane permeabilization (LMP) and Zinc (Zn2+ ) dynamics could be important modulators of such RSV-related effects and could globally represent a promising strategy to sensitize cancer cells to QCT treatment. This article is protected by copyright. All rights reserved.
Thematic Areas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 16134125
Author's mail: jordi.blanco@urv.cat miquel.mulero@urv.cat santi.garcia-vallve@urv.cat gerard.pujadas@urv.cat lluis.arola@urv.cat raul.beltran@urv.cat
Author identifier: 0000-0001-8016-0984 0000-0002-0348-7497 0000-0003-2598-8089 0000-0003-2767-1974 0000-0001-9691-1906
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Molecular Nutrition & Food Research. 62 (5):
APA: Tomas-Hernández S, Blanco J, Rojas C, Roca-Martínez J, Ojeda-Montes M, Beltrán-Debón R, Garcia-Vallvé S, Pujadas G, Arola L, Mulero M (2018). Resveratrol Potently Counteracts Quercetin Starvation-Induced Autophagy and Sensitizes HepG2 Cancer Cells to Apoptosis. Molecular Nutrition & Food Research, 62(5), -. DOI: 10.1002/mnfr.201700610
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV