Author, as appears in the article.: Tomas-Hernandez, Sarah; Blanco, Jordi; Rojas, Cristina; Roca-Martinez, Joel; Jose Ojeda-Montes, Maria; Beltran-Debon, Raul; Garcia-Vallve, Santiago; Pujadas, Gerard; Arola, Lluis; Mulero, Miquel
Department: Ciències Mèdiques Bàsiques; Bioquímica i Biotecnologia
URV's Author/s: Arola Ferrer, Luis Maria / Beltrán Debón, Raúl Alejandro / Blanco Pérez, Jordi / Garcia Vallve, Santiago / Mulero Abellán, Miguel / OJEDA MONTES, Mª JOSÉ / Pujadas Anguiano, Gerard / ROJAS DOMÍNGUEZ, CRISTINA / TOMAS HERNÁNDEZ, SARA
Keywords: Zinc; Ribosomal protein s6 kinases, 70-kda; Resveratrol; Quercetin; Protective role; Pathway; P70s6 kinase; Molecular-mechanisms; Lysosomal membrane permeabilization; Ischemia/reperfusion injury; Inhibition; In-vitro; Humans; Human leukemia-cells; Hepatic steatosis; Hep g2 cells; Heme oxygenase-1; Heme oxygenase 1; Endoplasmic reticulum stress; Ellagic acid; Death; Autophagy; Apoptosis; Amp-activated protein kinase; quercetin; p70s6 kinase; lysosomal membrane permeabilization; heme oxygenase 1; autophagy; apoptosis; amp-activated protein kinase
Abstract: Resveratrol (RSV) has been described as a potent antioxidant, anti-steatotic, and antitumor compound, and it has also been identified as a potent autophagy inducer. On the other hand, quercetin (QCT) is a dietary flavonoid with known antitumor, anti-inflammatory and antidiabetic effects. Additionally, QCT increases autophagy. To study the hypothetical synergistic effect of both compounds, we tested the combined effect of QCT and RSV on the autophagy process in HepG2 cells METHODS AND RESULTS: Autophagy was studied by western blotting, real-time RT-PCR, and cellular staining. Our results clearly indicate a bifunctional molecular effect of RSV. Both polyphenols were individually able to promote autophagy. Strikingly, when RSV was combined with QCT, it promoted a potent reduction of QCT-induced autophagy and influenced pro-apoptotic signaling. Conclusion: RSV acts differentially on the autophagic process depending on the cellular energetic state. We further characterized the molecular mechanisms related to this effect, and we observed that AMP-activated protein kinase (AMPK) phosphorylation, heme oxygenase 1 (HO-1) downregulation, lysosomal membrane permeabilization (LMP) and Zinc (Zn2+ ) dynamics could be important modulators of such RSV-related effects and could globally represent a promising strategy to sensitize cancer cells to QCT treatment. This article is protected by copyright. All rights reserved.
Thematic Areas: Saúde coletiva; Química; Nutrição; Medicina veterinaria; Medicina ii; Medicina i; Interdisciplinar; Food science & technology; Food science; Farmacia; Educação física; Ciências biológicas ii; Ciências biológicas i; Ciências agrárias i; Ciência de alimentos; Biotecnología; Biotechnology; Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 16134125
Author's mail: jordi.blanco@urv.cat; miquel.mulero@urv.cat; santi.garcia-vallve@urv.cat; gerard.pujadas@urv.cat; lluis.arola@urv.cat; raul.beltran@urv.cat
Record's date: 2025-02-24
Paper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.201700610
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Molecular Nutrition & Food Research. 62 (5): 1700610-
APA: Tomas-Hernandez, Sarah; Blanco, Jordi; Rojas, Cristina; Roca-Martinez, Joel; Jose Ojeda-Montes, Maria; Beltran-Debon, Raul; Garcia-Vallve, Santiago; P (2018). Resveratrol Potently Counteracts Quercetin Starvation-Induced Autophagy and Sensitizes HepG2 Cancer Cells to Apoptosis. Molecular Nutrition & Food Research, 62(5), 1700610-. DOI: 10.1002/mnfr.201700610
Article's DOI: 10.1002/mnfr.201700610
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV