Author, as appears in the article.: Fernandez-Castillejo, Sara; Formentin, Pilar; Catalan, Ursula; Pallares, Josep; Marsal, Lluis F; Sola, Rosa
Department: Medicina i Cirurgia Enginyeria Electrònica, Elèctrica i Automàtica
URV's Author/s: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / Formentín Vallés, Pilar / Marsal Garví, Luis Francisco / Pallarès Marzal, Josep / Solà Alberich, Rosa Maria
Keywords: Silicon Microgrooves Human aortic endothelial cells (haecs) Contact guidance Cell morphology microgrooves human aortic endothelial cells (haecs) contact guidance cell morphology
Abstract: Background: Micro- and nanoscale substrates have been fabricated in order to study the influence of the topography on the cellular response. The aim of this work was to prepare different collagen-coated silicon substrates displaying grooves and ridges to mimic the aligned and elongated endothelium found in linear vessels, and to use them as substrates to study cell growth and behaviour. Results: The influence of groove-shaped substrates on cell adhesion, morphology and proliferation were assessed, by comparing them to flat silicon substrates, used as control condition. Using human aortic endothelial cells, microscopy images demonstrate that the cellular response is different depending on the silicon surface, when it comes to cell adhesion, morphology (alignment, circularity and filopodia presence) and proliferation. Moreover, these structures exerted no cytotoxic effect. Conclusion: The results suggest that topographical patterning influences cell response. Silicon groove substrates can be used in developing medical devices with microscale features to mimic the endothelium in lineal vessels.
Thematic Areas: Química Physics, applied Physics and astronomy (miscellaneous) Physics and astronomy (all) Nanoscience and nanotechnology Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) Interdisciplinar General physics and astronomy General materials science Engenharias iv Engenharias iii Electrical and electronic engineering Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 21904286
Author's mail: ursula.catalan@urv.cat josep.pallares@urv.cat rosa.sola@urv.cat lluis.marsal@urv.cat pilar.formentin@urv.cat pilar.formentin@urv.cat
Author identifier: 0000-0001-8884-9823 0000-0001-7221-5383 0000-0002-8359-235X 0000-0002-5976-1408
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.beilstein-journals.org/bjnano/articles/8/72
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Beilstein Journal Of Nanotechnology. 8 (1): 675-681
APA: Fernandez-Castillejo, Sara; Formentin, Pilar; Catalan, Ursula; Pallares, Josep; Marsal, Lluis F; Sola, Rosa (2017). Silicon microgrooves for contact guidance of human aortic endothelial cells. Beilstein Journal Of Nanotechnology, 8(1), 675-681. DOI: 10.3762/bjnano.8.72
Article's DOI: 10.3762/bjnano.8.72
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications
Repositori URV