Author, as appears in the article.: Catalán Ú, López de Las Hazas MC, Rubió L, Fernández-Castillejo S, Pedret A, de la Torre R, Motilva MJ, Solà R.
Department: Medicina i Cirurgia
URV's Author/s: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / Pedret Figuerola, Anna / Solà Alberich, Rosa Maria
Keywords: atherosclerosis caco-2 human aortic endothelial cells hydroxytyrosol Atherosclerosis Caco 2 Caco-2 Human aortic endothelial cells Hydroxytyrosol Hydroxytyrosol metabolites
Abstract: Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due to an extensive first pass phase II metabolism. Therefore, it is necessary to find strategies to obtain HT metabolites and to demonstrate their protective role against the endothelial dysfunction. Biosynthesis of the main plasmatic HT metabolites was performed through Caco-2 cells. The bioactivity of HT and the mixture of metabolites was tested at physiological concentrations (1, 2, 5 and 10 μM) in human aortic endothelial cells (HAEC) co-incubated with TNF-α (10 ng/mL) for 18 and 24 h. After the incubations, cells and media were analyzed to test possible deconjugation of metabolites or conjugation of HT. Both HT and metabolites significantly reduced the secretion of E-selectin, P-selectin, ICAM-1 and VCAM-1, but only HT metabolites further reduced MCP-1 at 24 h. HT underwent a conjugation process after incubation leading to its main metabolites in a dose-dependent manner. Physiological HT metabolites, synthetized for the first time by using an intestinal cell model, might be the responsible in part for the protection against endothelial dysfunction. This article is protected by copyright.
Thematic Areas: Astronomia / física Biotechnology Biotecnología Ciência de alimentos Ciências agrárias i Ciências biológicas i Ciências biológicas ii Educação física Farmacia Food science Food science & technology Interdisciplinar Medicina i Medicina ii Medicina veterinaria Nutrição Química Saúde coletiva
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: rosa.sola@urv.cat anna.pedret@urv.cat ursula.catalan@urv.cat
Author identifier: 0000-0002-8359-235X 0000-0002-5327-932X 0000-0001-8884-9823
Record's date: 2023-02-18
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201500361
Papper original source: Molecular Nutrition & Food Research. 59 (12): 2523-2536
APA: Catalán Ú, López de Las Hazas MC, Rubió L, Fernández-Castillejo S, Pedret A, de la Torre R, Motilva MJ, Solà R. (2015). Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells. Molecular Nutrition & Food Research, 59(12), 2523-2536. DOI: 10.1002/mnfr.201500361
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1002/mnfr.201500361
Entity: Universitat Rovira i Virgili
Journal publication year: 2015
Publication Type: Journal Publications
Repositori URV