Articles producció científica> Medicina i Cirurgia

Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells

  • Identification data

    Identifier: imarina:3657585
    Authors:
    Catalán Ú, López de Las Hazas MC, Rubió L, Fernández-Castillejo S, Pedret A, de la Torre R, Motilva MJ, Solà R.
    Abstract:
    Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due to an extensive first pass phase II metabolism. Therefore, it is necessary to find strategies to obtain HT metabolites and to demonstrate their protective role against the endothelial dysfunction. Biosynthesis of the main plasmatic HT metabolites was performed through Caco-2 cells. The bioactivity of HT and the mixture of metabolites was tested at physiological concentrations (1, 2, 5 and 10 μM) in human aortic endothelial cells (HAEC) co-incubated with TNF-α (10 ng/mL) for 18 and 24 h. After the incubations, cells and media were analyzed to test possible deconjugation of metabolites or conjugation of HT. Both HT and metabolites significantly reduced the secretion of E-selectin, P-selectin, ICAM-1 and VCAM-1, but only HT metabolites further reduced MCP-1 at 24 h. HT underwent a conjugation process after incubation leading to its main metabolites in a dose-dependent manner. Physiological HT metabolites, synthetized for the first time by using an intestinal cell model, might be the responsible in part for the protection against endothelial dysfunction. This article is protected by copyright.
  • Others:

    Author, as appears in the article.: Catalán Ú, López de Las Hazas MC, Rubió L, Fernández-Castillejo S, Pedret A, de la Torre R, Motilva MJ, Solà R.
    Department: Medicina i Cirurgia
    URV's Author/s: Catalán Santos, Úrsula / FERNÁNDEZ CASTILLEJO, SARA / Pedret Figuerola, Anna / Solà Alberich, Rosa Maria
    Keywords: Hydroxytyrosol metabolites Hydroxytyrosol Human aortic endothelial cells Caco-2 Caco 2 Atherosclerosis hydroxytyrosol human aortic endothelial cells caco-2 atherosclerosis
    Abstract: Hydroxytyrosol (HT) is the major phenolic compound in virgin olive oil (VOO) in free and conjugated forms that may exert health benefits against atherosclerosis. The native form of HT is undetectable in plasma due to an extensive first pass phase II metabolism. Therefore, it is necessary to find strategies to obtain HT metabolites and to demonstrate their protective role against the endothelial dysfunction. Biosynthesis of the main plasmatic HT metabolites was performed through Caco-2 cells. The bioactivity of HT and the mixture of metabolites was tested at physiological concentrations (1, 2, 5 and 10 μM) in human aortic endothelial cells (HAEC) co-incubated with TNF-α (10 ng/mL) for 18 and 24 h. After the incubations, cells and media were analyzed to test possible deconjugation of metabolites or conjugation of HT. Both HT and metabolites significantly reduced the secretion of E-selectin, P-selectin, ICAM-1 and VCAM-1, but only HT metabolites further reduced MCP-1 at 24 h. HT underwent a conjugation process after incubation leading to its main metabolites in a dose-dependent manner. Physiological HT metabolites, synthetized for the first time by using an intestinal cell model, might be the responsible in part for the protection against endothelial dysfunction. This article is protected by copyright.
    Thematic Areas: Saúde coletiva Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: ursula.catalan@urv.cat anna.pedret@urv.cat rosa.sola@urv.cat
    Author identifier: 0000-0001-8884-9823 0000-0002-5327-932X 0000-0002-8359-235X
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://onlinelibrary.wiley.com/doi/10.1002/mnfr.201500361
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Molecular Nutrition & Food Research. 59 (12): 2523-2536
    APA: Catalán Ú, López de Las Hazas MC, Rubió L, Fernández-Castillejo S, Pedret A, de la Torre R, Motilva MJ, Solà R. (2015). Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells. Molecular Nutrition & Food Research, 59(12), 2523-2536. DOI: 10.1002/mnfr.201500361
    Article's DOI: 10.1002/mnfr.201500361
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2015
    Publication Type: Journal Publications
  • Keywords:

    Biotechnology,Food Science,Food Science & Technology
    Hydroxytyrosol metabolites
    Hydroxytyrosol
    Human aortic endothelial cells
    Caco-2
    Caco 2
    Atherosclerosis
    hydroxytyrosol
    human aortic endothelial cells
    caco-2
    atherosclerosis
    Saúde coletiva
    Química
    Nutrição
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Food science & technology
    Food science
    Farmacia
    Educação física
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biotechnology
    Astronomia / física
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