Author, as appears in the article.: Basaure P, Guardia-Escote L, Cabré M, Peris-Sampedro F, Sánchez-Santed F, Domingo JL, Colomina MT.
Department: Psicologia Ciències Mèdiques Bàsiques Bioquímica i Biotecnologia
URV's Author/s: BASAURE GARCÍA, PÍA ISABEL / Cabré Bargalló, Maria / Colomina Fosch, Maria Teresa / Domingo Roig, José Luis / Guardia Escoté, Laia
Keywords: Vesicular acetylcholine transport proteins Sex differences Prosencephalon Polymorphism, genetic Pesticide Paraoxonase Oxidative stress Mice, transgenic Mice, inbred c57bl Mice Male Litter size Insecticides Inactivation, metabolic Genotype Female Development Cholinergic system Choline o-acetyltransferase Chlorpyrifos Butyrylcholinesterase Aryldialkylphosphatase Apolipoprotein e4 Apolipoprotein e3 Apoe Animals, newborn Animals Alpha7 nicotinic acetylcholine receptor Acetylcholinesterase pesticide paraoxonase development cholinergic system apoe
Abstract: Chlorpyrifos (CPF) is one of the most commonly used organophosphate pesticides in the world. Our previous results described that apolipoprotein E (APOE) polymorphisms are a source of individual differences in susceptibility to CPF. The aim of this study was to assess the physical and biochemical effects of postnatal exposure to CPF in the apoE targeted replacement mouse model. Mice were exposed to CPF at 0 or 1 mg/kg/day from postnatal day 10-15. Physical development, plasma and forebrain cholinesterase (ChE) activity and gene expression in liver and forebrain were evaluated. CPF exposure delays physical maturation and decreases the expression of choline acetyltransferase, α4-subunit and the α7 receptor. CPF decreases the expression of vesicular acetylcholine transporter (VAChT) mRNA in the forebrain only in apoE3 mice. The expression of paraoxonase-2 in the forebrain was also influenced by APOE genotype and CPF. Differences between genotypes were observed in litter size, ChE activity, expression of butyrylcholinesterase and paraoxonase-1 in liver and variants of acetylcholinesterase, VAChT and the α7 receptor in the forebrain. These results support that there are different vulnerabilities to postnatal CPF exposure according to the APOE polymorphism, which in turn affects the cholinergic system and defenses to oxidative stress.
Thematic Areas: Toxicology Saúde coletiva Química Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Food science & technology Food science Farmacia Ensino Engenharias iv Engenharias ii Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 02786915
Author's mail: maria.cabre@urv.cat joseluis.domingo@urv.cat mariateresa.colomina@urv.cat
Author identifier: 0000-0003-4124-8603 0000-0001-6647-9470 0000-0002-5619-4874
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Food And Chemical Toxicology. 118 42-52
APA: Basaure P, Guardia-Escote L, Cabré M, Peris-Sampedro F, Sánchez-Santed F, Domingo JL, Colomina MT. (2018). Postnatal chlorpyrifos exposure and apolipoprotein E (APOE) genotype differentially affect cholinergic expression and developmental parameters in transgenic mice. Food And Chemical Toxicology, 118(), 42-52. DOI: 10.1016/j.fct.2018.04.065
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV