Author, as appears in the article.: Basaure, Pia; Guardia-Escote, Laia; Cabre, Maria; Peris-Sampedro, Fiona; Sanchez-Santed, Fernando; Domingo, Jose L; Teresa Colomina, Maria
Department: Psicologia; Ciències Mèdiques Bàsiques; Bioquímica i Biotecnologia
URV's Author/s: BASAURE GARCÍA, PÍA ISABEL / Cabré Bargalló, Maria / Colomina Fosch, Maria Teresa / Domingo Roig, José Luis / Guardia Escoté, Laia
Keywords: Vesicular acetylcholine transport proteins; Sex differences; Prosencephalon; Polymorphism, genetic; Pesticide; Paraoxonase; Oxidative stress; Mice, transgenic; Mice, inbred c57bl; Mice; Male; Litter size; Insecticides; Inactivation, metabolic; Genotype; Female; Development; Cholinergic system; Choline o-acetyltransferase; Chlorpyrifos; Butyrylcholinesterase; Aryldialkylphosphatase; Apolipoprotein e4; Apolipoprotein e3; Apoe; Animals, newborn; Animals; Alpha7 nicotinic acetylcholine receptor; Acetylcholinesterase; pesticide; paraoxonase; development; cholinergic system; apoe
Abstract: Chlorpyrifos (CPF) is one of the most commonly used organophosphate pesticides in the world. Our previous results described that apolipoprotein E (APOE) polymorphisms are a source of individual differences in susceptibility to CPF. The aim of this study was to assess the physical and biochemical effects of postnatal exposure to CPF in the apoE targeted replacement mouse model. Mice were exposed to CPF at 0 or 1 mg/kg/day from postnatal day 10-15. Physical development, plasma and forebrain cholinesterase (ChE) activity and gene expression in liver and forebrain were evaluated. CPF exposure delays physical maturation and decreases the expression of choline acetyltransferase, α4-subunit and the α7 receptor. CPF decreases the expression of vesicular acetylcholine transporter (VAChT) mRNA in the forebrain only in apoE3 mice. The expression of paraoxonase-2 in the forebrain was also influenced by APOE genotype and CPF. Differences between genotypes were observed in litter size, ChE activity, expression of butyrylcholinesterase and paraoxonase-1 in liver and variants of acetylcholinesterase, VAChT and the α7 receptor in the forebrain. These results support that there are different vulnerabilities to postnatal CPF exposure according to the APOE polymorphism, which in turn affects the cholinergic system and defenses to oxidative stress.
Thematic Areas: Toxicology; Saúde coletiva; Química; Odontología; Nutrição; Medicine (miscellaneous); Medicina veterinaria; Medicina iii; Medicina ii; Medicina i; Materiais; Interdisciplinar; Food science & technology; Food science; Farmacia; Ensino; Engenharias iv; Engenharias ii; Enfermagem; Educação física; Ciências biológicas iii; Ciências biológicas ii; Ciências biológicas i; Ciências ambientais; Ciências agrárias i; Ciência de alimentos; Biotecnología; Biodiversidade; Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 02786915
Author's mail: maria.cabre@urv.cat; joseluis.domingo@urv.cat; mariateresa.colomina@urv.cat
Record's date: 2025-02-08
Paper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S0278691518302898?via%3Dihub
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Food And Chemical Toxicology. 118 42-52
APA: Basaure, Pia; Guardia-Escote, Laia; Cabre, Maria; Peris-Sampedro, Fiona; Sanchez-Santed, Fernando; Domingo, Jose L; Teresa Colomina, Maria (2018). Postnatal chlorpyrifos exposure and apolipoprotein E (APOE) genotype differentially affect cholinergic expression and developmental parameters in transgenic mice. Food And Chemical Toxicology, 118(), 42-52. DOI: 10.1016/j.fct.2018.04.065
Article's DOI: 10.1016/j.fct.2018.04.065
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV