Author, as appears in the article.: Pérez-Cataluña A; Salas-Massó N; Diéguez A; Balboa S; Lema A; Romalde J; Figueras M
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Figueras Salvat, María Josefa / PÉREZ CATALUÑA, ALBA
Keywords: aliiarcobacter gen. nov. arcobacter haloarcobacter gen. nov. malacobacter gen. nov. poseidonibacter gen. nov. pseudoarcobacter gen. nov. Aliiarcobacter gen. nov. Arcobacter Haloarcobacter gen. nov. Malacobacter gen. nov. Poseidonibacter gen. nov. Pseudoarcobacter gen. nov. Taxonomic criteria
Abstract: © 2018 Pérez-Cataluña, Salas-Massó, Diéguez, Balboa, Lema, Romalde and Figueras. Since the description of the genus Arcobacter in 1991, a total of 27 species have been described, although some species have shown 16S rRNA similarities below 95%, which is the cut-off that usually separates species that belong to different genera. The objective of the present study was to reassess the taxonomy of the genus Arcobacter using information derived from the core genome (286 genes), a Multilocus Sequence Analysis (MLSA) with 13 housekeeping genes, as well as different genomic indexes like Average Nucleotide Identity (ANI), in silico DNA-DNA hybridization (isDDH), Average Amino-acid Identity (AAI), Percentage of Conserved Proteins (POCPs), and Relative Synonymous Codon Usage (RSCU). The study included a total of 39 strains that represent all the 27 species included in the genus Arcobacter together with 13 strains that are potentially new species, and the analysis of 57 genomes. The different phylogenetic analyses showed that the Arcobacter species grouped into four clusters. In addition, A. lekithochrous and the candidatus species 'A. aquaticus' appeared, as did A. nitrofigilis, the type species of the genus, in separate branches. Furthermore, the genomic indices ANI and isDDH not only confirmed that all the species were well-defined, but also the coherence of the clusters. The AAI and POCP values showed intra-cluster ranges above the respective cut-off values of 60% and 50% described for species belonging to the same genus. Phenotypic analysis showed that certain test combinations could allow the differentiation of the four clusters and the three orphan species established by the phylogenetic and genomic analyses. The origin of the strains showed that each of the clusters embraced species recovered from a common or related environment. The results obtained enable the division of the current genus Arcobacter in at least seven different genera, for which the names Arcobacter, Aliiarcobacter gen. nov., Pseudoarcobacter gen. nov., Haloarcobacter gen. nov., Malacobacter gen. nov., Poseidonibacter gen. nov., and Candidate 'Arcomarinus' gen. nov. are proposed.
Thematic Areas: Astronomia / física Biodiversidade Biotecnología Ciência da computação Ciência de alimentos Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Engenharias i Engenharias ii Engenharias iii Ensino Farmacia Geociências Geografía Interdisciplinar Matemática / probabilidade e estatística Materiais Medicina i Medicina ii Medicina veterinaria Microbiology Microbiology (medical) Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariajose.figueras@urv.cat
ISSN: 1664302X
Author identifier: 0000-0002-2268-8980
Record's date: 2023-02-22
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2018.02077/full
Papper original source: Frontiers In Microbiology. 9 (SEP): 2077-
APA: Pérez-Cataluña A; Salas-Massó N; Diéguez A; Balboa S; Lema A; Romalde J; Figueras M (2018). Revisiting the taxonomy of the genus arcobacter: Getting order from the chaos. Frontiers In Microbiology, 9(SEP), 2077-. DOI: 10.3389/fmicb.2018.02077
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.3389/fmicb.2018.02077
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV