Author, as appears in the article.: Vincent A; Fernández-Bravo A; Sanchis M; Mayayo E; Figueras M; Charette S
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Fernández Bravo, Ana / Figueras Salvat, María Josefa / Mayayo Artal, Emilio Manuel / Sanchis Talón, Marta
Keywords: aeromonas salmonicida infection necrotizing fasciitis pathogenicity type iii secretion systems Aeromonas salmonicida Genes Identification Iii secretion system Infection Necrotizing fasciitis Pathogenicity Resistance Temperature Type iii secretion systems Whole genome sequencing
Abstract: © 2018 Elsevier B.V. The bacterium Aeromonas salmonicida is known since long time as a major fish pathogen unable to grow at 37 °C. However, some cases of human infection by putative mesophilic A. salmonicida have been reported. The goal of the present study is to examine two clinical cases of human infection by A. salmonicida in Spain and to investigate the pathogenicity in mammals of selected mesophilic A. salmonicida strains. An evaluation of the pathogenicity in a mouse model of clinical and environmental A. salmonicida strains was performed. The genomes of the strains were sequenced and analyzed in order to find the virulence determinants of these strains. The experimental infection in mice showed a gradient in the virulence of these strains and that some of them can cause necrotizing fasciitis and tissue damage in the liver. In addition to demonstrating significant genomic diversity among the strains studied, bioinformatics analyses permitted also to shed light on crucial elements for the virulence of the strains, like the presence of a type III secretion system in the one that caused the highest mortality in the experimental infection. Clinicians and microbiologists should consider these results for the inclusion of A. salmonicida in diagnosis tests since it is now clear that some mesophilic strains are also pathogens for humans.
Thematic Areas: Biodiversidade Biotecnología Ciência da computação Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Ecology, evolution, behavior and systematics Enfermagem Ensino Farmacia General medicine Genetics Infectious diseases Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Microbiology Microbiology (medical) Molecular biology Planejamento urbano e regional / demografia Saúde coletiva Zootecnia / recursos pesqueiros
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariajose.figueras@urv.cat emilio.mayayo@urv.cat marta.sanchis@urv.cat ana.fernandez@urv.cat
ISSN: 15671348
Author identifier: 0000-0002-2268-8980 0000-0003-4485-3809 0000-0001-7251-6792 0000-0001-7637-5958
Record's date: 2023-02-22
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1567134818304775?via%3Dihub
Papper original source: Infection Genetics And Evolution. 68 1-9
APA: Vincent A; Fernández-Bravo A; Sanchis M; Mayayo E; Figueras M; Charette S (2019). Investigation of the virulence and genomics of Aeromonas salmonicida strains isolated from human patients. Infection Genetics And Evolution, 68(), 1-9. DOI: 10.1016/j.meegid.2018.11.019
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1016/j.meegid.2018.11.019
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV