Author, as appears in the article.: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M.
Department: Bioquímica i Biotecnologia
URV's Author/s: Ardévol Grau, Anna / Arola Ferrer, Luis Maria / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Keywords: Transepithelial activity Swine Peptide yy Intestines Intestinal mucosa In vitro techniques Gut hormone secretion Food bioactives Female Ex vivo model Enteroendocrine system Enteroendocrine cells Biological assay Animals gut hormone secretion food bioactives ex vivo model enteroendocrine system
Abstract: The enteroendocrine system coordinates gastrointestinal (GI) tract functionality and the whole organism. However, the scarcity of enteroendocrine cells and their scattered distribution make them difficult to study. Here, we glued segments of the GI wall of pigs to a silicon tube, keeping the apical and the basolateral sides separate. The fact that there was less than 1% of 70-kDa fluorescein isothiocyanate (FITC)-dextran on the basolateral side proved that the gluing was efficient. Since the lactate dehydrogenase leakage at basolateral side was lower than 0.1% (1.40 ± 0.17 nKatals) it proved that the tissue was viable. The intestinal barrier function was maintained as it is in segments mounted in Ussing chambers (the amount of Lucifer Yellow crossing it, was similar between them; respectively, % LY, 0.48 ± 0.13; 0.52 ± 0.09; p > 0.05). Finally, apical treatments with two different extract produced differential basolateral enterohormone secretions (basolateral PYY secretion vs control; animal extract, 0.35 ± 0.16; plant extract, 2.5 ± 0.74; p < 0.05). In conclusion, we report an ex vivo system called "Ap-to-Bas" for assaying vectorial transepithelial processes that makes it possible to work with several samples at the same time. It is an optimal device for enterohormone studies in the intestine.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Planejamento urbano e regional / demografia Odontología Nutrição Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry General agricultural and biological sciences Food science & technology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física Agriculture, multidisciplinary Agriculture Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all)
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 00218561
Author's mail: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat lluis.arola@urv.cat
Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0003-2767-1974
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://pubs.acs.org/doi/10.1021/acs.jafc.8b03046
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Agricultural And Food Chemistry. 66 (44): 11622-11629
APA: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M. (2018). Novel ex Vivo Experimental Setup to Assay the Vectorial Transepithelial Enteroendocrine Secretions of Different Intestinal Segments.. Journal Of Agricultural And Food Chemistry, 66(44), 11622-11629. DOI: 10.1021/acs.jafc.8b03046
Article's DOI: 10.1021/acs.jafc.8b03046
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications