Articles producció científica> Bioquímica i Biotecnologia

Novel ex Vivo Experimental Setup to Assay the Vectorial Transepithelial Enteroendocrine Secretions of Different Intestinal Segments.

  • Identification data

    Identifier: imarina:4684392
    Authors:
    Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M.
    Abstract:
    The enteroendocrine system coordinates gastrointestinal (GI) tract functionality and the whole organism. However, the scarcity of enteroendocrine cells and their scattered distribution make them difficult to study. Here, we glued segments of the GI wall of pigs to a silicon tube, keeping the apical and the basolateral sides separate. The fact that there was less than 1% of 70-kDa fluorescein isothiocyanate (FITC)-dextran on the basolateral side proved that the gluing was efficient. Since the lactate dehydrogenase leakage at basolateral side was lower than 0.1% (1.40 ± 0.17 nKatals) it proved that the tissue was viable. The intestinal barrier function was maintained as it is in segments mounted in Ussing chambers (the amount of Lucifer Yellow crossing it, was similar between them; respectively, % LY, 0.48 ± 0.13; 0.52 ± 0.09; p > 0.05). Finally, apical treatments with two different extract produced differential basolateral enterohormone secretions (basolateral PYY secretion vs control; animal extract, 0.35 ± 0.16; plant extract, 2.5 ± 0.74; p < 0.05). In conclusion, we report an ex vivo system called "Ap-to-Bas" for assaying vectorial transepithelial processes that makes it possible to work with several samples at the same time. It is an optimal device for enterohormone studies in the intestine.
  • Others:

    Author, as appears in the article.: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M.
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Ardévol Grau, Anna / Arola Ferrer, Luis Maria / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
    Keywords: Transepithelial activity Swine Peptide yy Intestines Intestinal mucosa In vitro techniques Gut hormone secretion Food bioactives Female Ex vivo model Enteroendocrine system Enteroendocrine cells Biological assay Animals gut hormone secretion food bioactives ex vivo model enteroendocrine system
    Abstract: The enteroendocrine system coordinates gastrointestinal (GI) tract functionality and the whole organism. However, the scarcity of enteroendocrine cells and their scattered distribution make them difficult to study. Here, we glued segments of the GI wall of pigs to a silicon tube, keeping the apical and the basolateral sides separate. The fact that there was less than 1% of 70-kDa fluorescein isothiocyanate (FITC)-dextran on the basolateral side proved that the gluing was efficient. Since the lactate dehydrogenase leakage at basolateral side was lower than 0.1% (1.40 ± 0.17 nKatals) it proved that the tissue was viable. The intestinal barrier function was maintained as it is in segments mounted in Ussing chambers (the amount of Lucifer Yellow crossing it, was similar between them; respectively, % LY, 0.48 ± 0.13; 0.52 ± 0.09; p > 0.05). Finally, apical treatments with two different extract produced differential basolateral enterohormone secretions (basolateral PYY secretion vs control; animal extract, 0.35 ± 0.16; plant extract, 2.5 ± 0.74; p < 0.05). In conclusion, we report an ex vivo system called "Ap-to-Bas" for assaying vectorial transepithelial processes that makes it possible to work with several samples at the same time. It is an optimal device for enterohormone studies in the intestine.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Planejamento urbano e regional / demografia Odontología Nutrição Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry General agricultural and biological sciences Food science & technology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física Agriculture, multidisciplinary Agriculture Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00218561
    Author's mail: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat lluis.arola@urv.cat
    Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0003-2767-1974
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://pubs.acs.org/doi/10.1021/acs.jafc.8b03046
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Journal Of Agricultural And Food Chemistry. 66 (44): 11622-11629
    APA: Ginés I, Gil-Cardoso K, Robles P, Arola L, Terra X, Blay M, Ardévol A, Pinent M. (2018). Novel ex Vivo Experimental Setup to Assay the Vectorial Transepithelial Enteroendocrine Secretions of Different Intestinal Segments.. Journal Of Agricultural And Food Chemistry, 66(44), 11622-11629. DOI: 10.1021/acs.jafc.8b03046
    Article's DOI: 10.1021/acs.jafc.8b03046
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    Publication Type: Journal Publications
  • Keywords:

    Agricultural and Biological Sciences (Miscellaneous),Agriculture,Agriculture, Multidisciplinary,Chemistry (Miscellaneous),Chemistry, Applied,Food Science & Technology
    Transepithelial activity
    Swine
    Peptide yy
    Intestines
    Intestinal mucosa
    In vitro techniques
    Gut hormone secretion
    Food bioactives
    Female
    Ex vivo model
    Enteroendocrine system
    Enteroendocrine cells
    Biological assay
    Animals
    gut hormone secretion
    food bioactives
    ex vivo model
    enteroendocrine system
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Planejamento urbano e regional / demografia
    Odontología
    Nutrição
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General medicine
    General chemistry
    General agricultural and biological sciences
    Food science & technology
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Educação
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, applied
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
    Astronomia / física
    Agriculture, multidisciplinary
    Agriculture
    Agricultural and biological sciences (miscellaneous)
    Agricultural and biological sciences (all)
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