Author, as appears in the article.: Rodriguez-Calvo, Ricardo; Girona, Josefa; Rodriguez, Marina; Samino, Sara; Barroso, Emma; de Gonzalo-Calvo, David; Guaita-Esteruelas, Sandra; Heras, Mercedes; van der Meer, Rutger W; Lamb, Hildo J; Yanes, Oscar; Correig, Xavier; Llorente-Cortes, Vicenta; Vazquez-Carrera, Manuel; Masana, Lluis
Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques
URV's Author/s: Correig Blanchar, Francesc Xavier / Girona Tell, Josefa / GUAITA ESTERUELAS, SANDRA / HERAS IBAÑEZ, MERCEDES / Masana Marín, Luis / Rodríguez Calvo, Ricardo / Rodríguez Chacón, Matilde / SAMINO GENÉ, SARA / Yanes Torrado, Óscar
Keywords: Waist circumference Upregulation Unclassified drug Triglycerides Triglyceride content Triacylglycerol blood level Triacylglycerol Suppressor of cytokine signaling 3 Subcutaneous fat Steatosis Stat3 protein Signal transduction Resistin Pyruvate dehydrogenase kinase 4 Pyrazoles Pyrazole derivative Proton nuclear magnetic resonance Protein phosphorylation Protein inhibitor Protein expression level Protein blood level Phospholipid Non insulin dependent diabetes mellitus Myocardium Myocardial neutral lipid content Middle aged Mice, inbred c57bl Mice Metabolism Metabolic syndrome Messenger rna Male Low density lipoprotein cholesterol Lipid metabolism Leptin Left-ventricular mass Insulin-resistance Insulin resistance Insulin Humans High density lipoprotein cholesterol Hemoglobin a1c Heart-failure Glucose transporter 4 Glucose Female Fatty acid-binding proteins Fatty acid binding protein 4 Fatty acid binding protein Fatty acid Fabp4 protein, mouse Fabp4 protein, human Fabp4 Diet, high-fat Diabetic patient Diabetic cardiomyopathy Diabetic cardiomyopathies Diabetes mellitus, type 2 Coronary-artery-disease Controlled study Colecalciferol Cholesterol derivative Cell line Cardiac-function Cardiac muscle cell Cardiac muscle Cardiac lipotoxicity C57bl mouse Bms309403 Bms 309403 Blood biochemistry Blood Biphenyl derivative Biphenyl compounds Biomarkers Biological marker Article Animals Animal model Animal experiment Animal cell Animal Adipose-tissue Adiponectin Accumulation 2-(2'-(5-ethyl-3,4-diphenyl-1h-pyrazol-1-yl)biphenyl-3-yloxy)acetic acid insulin resistance fabp4 cardiac lipotoxicity bms309403
Abstract: © 2019 Elsevier Inc. Objective: Fatty acid binding protein 4 (FABP4) is an intracellular lipid chaperone involved in the crosstalk between adipose and peripheral tissues, and it contributes to widespread insulin resistance in cells, including cardiac cells. However, the role of this adipokine in regulating cardiac metabolism and myocardial neutral lipid content in patients with type 2 diabetes has not been elucidated. Methods: The impact of circulating FABP4 on the cardiac neutral lipid content was measured by proton magnetic resonance spectroscopy ( 1 H-MRS) in patients with type 2 diabetes. Additionally, circulating FABP4 and the cardiac triglyceride content were analysed in high-fat diet (HFD)-fed mice, and the impact of the exogenous FABP4 was explored in HL-1 cardiac cells. Results: Serum FABP4 levels were higher in type 2 diabetic patients compared to healthy individuals. Circulating FABP4 levels were associated with myocardial neutral lipid content in type 2 diabetic patients. In HFD-fed mice, both serum FABP4 and myocardial triglyceride content were increased. In FABP4-challenged HL-1 cells, extracellular FABP4 increased intracellular lipid accumulation, which led to impairment of the insulin-signalling pathway and reduced insulin-stimulated glucose uptake. However, these effects were partially reversed by FABP4 inhibition with BMS309403, which attenuated the intracellular lipid content and improved insulin signalling and insulin-stimulated glucose uptake. Conclusions: Taken together, our results identify FABP4 as a molecule involved in diabetic/lipid-induced cardiomyopathy and indicate that this molecule may be an emerging biomarker for diabetic cardiomyopathy-related disturbances, such as myocardial neutral lipid accumulation. Additionally, FABP4 inhibition may be a potential therapeutic target for metabolic-related cardiac dysfunctions.
Thematic Areas: Saúde coletiva Odontología Nutrição Medicina iii Medicina ii Medicina i Interdisciplinar General medicine Farmacia Engenharias ii Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Endocrinology Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología Antropologia / arqueologia
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 00260495
Author's mail: josefa.girona@urv.cat ricardo.rodriguez@urv.cat oscar.yanes@urv.cat matilde.rodriguez@urv.cat josefa.girona@urv.cat xavier.correig@urv.cat luis.masana@urv.cat
Author identifier: 0000-0002-6267-8779 0000-0003-3695-7157 0000-0002-6267-8779 0000-0002-6902-3054 0000-0002-0789-4954
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Metabolism-Clinical And Experimental. 96 12-21
APA: Rodriguez-Calvo, Ricardo; Girona, Josefa; Rodriguez, Marina; Samino, Sara; Barroso, Emma; de Gonzalo-Calvo, David; Guaita-Esteruelas, Sandra; Heras, M (2019). Fatty acid binding protein 4 (FABP4) as a potential biomarker reflecting myocardial lipid storage in type 2 diabetes. Metabolism-Clinical And Experimental, 96(), 12-21. DOI: 10.1016/j.metabol.2019.04.007
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV