Articles producció científica> Ciències Mèdiques Bàsiques

Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp.

  • Identification data

    Identifier: imarina:5127413
    Authors:
    Figueras, Maria JoséLevican, ArturoCollado, Luis
    Abstract:
    Background: Arcobacter spp. (family Campylobacteraceae) are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, using a single endonuclease (MseI). The number of characterised Arcobacter species has since risen to 17. The aim of the present study was to update the 16S rRNA-RFLP identification method to include all currently characterised species of Arcobacter. Results: Digestion of the 16S rRNA gene with the endonuclease MseI produced clear, distinctive patterns for 10 of the 17 species, while the remaining species shared a common or very similar RFLP pattern. Subsequent digestion of the 16S rRNA gene from these species with the endonucleases MnlI and/or BfaI generated species-specific RFLP patterns. Conclusions: 16S rRNA-RFLP analysis identified 17 Arcobacter spp. using either polyacrylamide or agarose gel electrophoresis. Microheterogeneities within the 16S rRNA gene, which interfered with the RFLP identification, were also documented for the first time in this genus, particularly in strains of Arcobacter cryaerophilus isolated from animal faeces and aborted foetuses.
  • Others:

    Author, as appears in the article.: Figueras, Maria José; Levican, Arturo; Collado, Luis;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Figueras Salvat, María Josefa
    Keywords: Taxonomy Sp nov. Polyacrylamide Pcr assay Multiplex Identification Helicobacter Gene Fragment-length-polymorphism Campylobacter Butzleri Arcobacter Agarose Aeromonas 16s rrna-rflp 16s rrna gene mutations
    Abstract: Background: Arcobacter spp. (family Campylobacteraceae) are ubiquitous zoonotic bacteria that are being increasingly recognised as a threat to human health. A previously published 16S rRNA-RFLP Arcobacter spp. identification method produced specific RFLP patterns for the six species described at that time, using a single endonuclease (MseI). The number of characterised Arcobacter species has since risen to 17. The aim of the present study was to update the 16S rRNA-RFLP identification method to include all currently characterised species of Arcobacter. Results: Digestion of the 16S rRNA gene with the endonuclease MseI produced clear, distinctive patterns for 10 of the 17 species, while the remaining species shared a common or very similar RFLP pattern. Subsequent digestion of the 16S rRNA gene from these species with the endonucleases MnlI and/or BfaI generated species-specific RFLP patterns. Conclusions: 16S rRNA-RFLP analysis identified 17 Arcobacter spp. using either polyacrylamide or agarose gel electrophoresis. Microheterogeneities within the 16S rRNA gene, which interfered with the RFLP identification, were also documented for the first time in this genus, particularly in strains of Arcobacter cryaerophilus isolated from animal faeces and aborted foetuses.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: mariajose.figueras@urv.cat
    Author identifier: 0000-0002-2268-8980
    Record's date: 2023-02-22
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-12-292
    Papper original source: Bmc Microbiology. 12 (292): 292-
    APA: Figueras, Maria José; Levican, Arturo; Collado, Luis; (2012). Updated 16S rRNA-RFLP method for the identification of all currently characterised Arcobacter spp.. Bmc Microbiology, 12(292), 292-. DOI: 10.1186/1471-2180-12-292
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1186/1471-2180-12-292
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2012
    Publication Type: Journal Publications
  • Keywords:

    Microbiology,Microbiology (Medical)
    Taxonomy
    Sp nov.
    Polyacrylamide
    Pcr assay
    Multiplex
    Identification
    Helicobacter
    Gene
    Fragment-length-polymorphism
    Campylobacter
    Butzleri
    Arcobacter
    Agarose
    Aeromonas
    16s rrna-rflp
    16s rrna gene mutations
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Microbiology (medical)
    Microbiology
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
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