Repositori URV

Author, as appears in the article.: Levican A, Figueras M

Department: Ciències Mèdiques Bàsiques

URV's Author/s: Figueras Salvat, María Josefa

Keywords: Sp nov. Skirrowii Shellfish Rflp method Pcr Molecular methods M-pcr M pcr Identification Helicobacter Gyra Diversity Cryaerophilus Comparison Campylobacter Arcobacter 23s rrna 16s rrna-rflp 16s rrna rflp

Abstract: Background: Bacteria belonging to the Arcobacter genus are emerging enteropathogens and potential zoonotic agents. Their taxonomy has evolved very rapidly, and there are presently 18 recorded species. The prevalence of species belonging to Arcobacter is underestimated because of the limitations of currently available methods for species identification. The aim of this study was to compare the performance of five PCR based methods that target regions of 16S rRNA, 23S rRNA or gyrA genes to identify Arcobacter species, and to review previous results reported in the literature using these methods. Results: The five tested methods were found not to be reliable. They misidentified between 16.8% and 67.4% of the studied strains; this was dependent upon the target regions of the tested genes. The worst results obtained were for the identification of Arcobacter cryaerophilus and Arcobacter butzleri when the 23S rRNA gene was used as the target. These species were confused with many non-targeted species. Conclusion: Our results suggest that the known diversity of Arcobacter spp. in different environments could be expanded if reliable identification methods are applied in future studies.

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: mariajose.figueras@urv.cat

Author identifier: 0000-0002-2268-8980

Record's date: 2023-02-22

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://bmcmicrobiol.biomedcentral.com/articles/10.1186/1471-2180-13-220

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Bmc Microbiology. 13 (1):

APA: Levican A, Figueras M (2013). Performance of five molecular methods for monitoring Arcobacter spp.. Bmc Microbiology, 13(1), -. DOI: 10.1186/1471-2180-13-220

Article's DOI: 10.1186/1471-2180-13-220

Entity: Universitat Rovira i Virgili

Journal publication year: 2013

Publication Type: Journal Publications