Author, as appears in the article.: Ceperuelo-Mallafre, Victoria; Ejarque, Miriam; Serena, Carolina; Duran, Xavier; Montori-Grau, Marta; Angel Rodriguez, Miguel; Yanes, Oscar; Nunez-Roa, Catalina; Roche, Kelly; Puthanveetil, Prasanth; Garrido-Sanchez, Lourdes; Saez, Enrique; Tinahones, Francisco J; Garcia-Roves, Pablo M; Ma Gomez-Foix, Anna; Saltiel, Alan R; Vendrell, Joan; Fernandez-Veledo, Sonia
Department: Ciències Mèdiques Bàsiques Medicina i Cirurgia Enginyeria Electrònica, Elèctrica i Automàtica
URV's Author/s: Ceperuelo Mallafré, Maria Victoria / Fernandez Veledo, Sonia / Serena Perelló, Carolina / Vendrell Ortega, Juan José / Yanes Torrado, Óscar
Keywords: Targeting subunits Retinal-pigment epithelium Proinflammatory cytokine Obesity Necrosis-factor-alpha Macrophage Lipid-metabolism Insulin-resistance Insulin resistance Glycogen Glucose-transport Endoplasmic-reticulum stress Diabetes Binding domain Autophagy Adipose tissue Adipocyte Activated protein-kinase macrophage insulin resistance glycogen autophagy adipocyte
Abstract: Objective: Glycogen metabolism has emerged as a mediator in the control of energy homeostasis and studies in murine models reveal that adipose tissue might contain glycogen stores. Here we investigated the physio(patho)logical role of glycogen in human adipose tissue in the context of obesity and insulin resistance. Methods: We studied glucose metabolic flux of hypoxic human adipoctyes by nuclear magnetic resonance and mass spectrometry-based metabolic approaches. Glycogen synthesis and glycogen content in response to hypoxia was analyzed in human adipocytes and macrophages. To explore the metabolic effects of enforced glycogen deposition in adipocytes and macrophages, we overexpressed PTG, the only glycogen-associated regulatory subunit (PP1-GTS) reported in murine adipocytes. Adipose tissue gene expression analysis was performed on wild type and homozygous PTG KO male mice. Finally, glycogen metabolism gene expression and glycogen accumulation was analyzed in adipose tissue, mature adipocytes and resident macrophages from lean and obese subjects with different degrees of insulin resistance in 2 independent cohorts. Results: We show that hypoxia modulates glucose metabolic flux in human adipocytes and macrophages and promotes glycogenesis. Enforced glycogen deposition by overexpression of PTG re-orients adipocyte secretion to a pro-inflammatory response linked to insulin resistance and monocyte/lymphocyte migration. Furthermore, glycogen accumulation is associated with inhibition of mTORC1 signaling and increased basal autophagy flux, correlating with greater leptin release in glycogen-loaded adipocytes. PTG-KO mice have reduced expression of key inflammatory genes in adipose tissue and PTG overexpression in M0 macrophages induces a pro-inflammatory and glycolytic M1 phenotype. Increased glycogen synthase expression correlates with glycogen deposition in subcutaneous adipose tissue of obese patients. Glycogen content in subcutaneous mature adipocytes is associated with BMI and leptin expression. Conclusion: Our data establish glycogen mishandling in adipose tissue as a potential key feature of inflammatory-related metabolic stress in human obesity.
Thematic Areas: Molecular biology Endocrinology & metabolism Ciências biológicas ii Cell biology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 22128778
Author's mail: oscar.yanes@urv.cat carolina.serena@urv.cat victoria.ceperuelo@urv.cat sonia.fernandez@urv.cat juanjose.vendrell@urv.cat
Author identifier: 0000-0003-3695-7157 0000-0002-4460-9761 0000-0003-2906-3788 0000-0002-6994-6115
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.sciencedirect.com/science/article/pii/S2212877815001969
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Molecular Metabolism. 5 (1): 5-18
APA: Ceperuelo-Mallafre, Victoria; Ejarque, Miriam; Serena, Carolina; Duran, Xavier; Montori-Grau, Marta; Angel Rodriguez, Miguel; Yanes, Oscar; Nunez-Roa, (2016). Adipose tissue glycogen accumulation is associated with obesity-linked inflammation in humans. Molecular Metabolism, 5(1), 5-18. DOI: 10.1016/j.molmet.2015.10.001
Article's DOI: 10.1016/j.molmet.2015.10.001
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV