Author, as appears in the article.: Martinez-Micaelo, N.; Gonzalez-Abuin, N.; Pinent, M.; Ardevol, A.; Blay, M.;

Department: Bioquímica i Biotecnologia

URV's Author/s: Ardèvol Grau, Anna / Blay Olivé, Maria Teresa / GONZÁLEZ ABUÍN, NOEMÍ / Martínez Micaelo, Nieves Beatriz / Pinent Armengol, Montserrat

Keywords: Fatty acids Dietary supplements

Abstract: The Nod-like receptor protein 3 (NLRP3) inflammasome is considered to be a pivotal host platform responsible for sensing of exogenous and endogenous danger signals, including those generated as a result of metabolic dysregulation, and for the subsequent, IL-1β-mediated orchestration of inflammatory and innate immunity responses. In this way, although the molecular link between diet-induced obesity and inflammasome activation is still unclear, free fatty acids (FFA) have been proposed as a triggering event. We report that dietary fatty acid (FA) composition is sensed by the NLRP3 inflammasome in human macrophages. For this purpose, we have analysed three roles of FA supplementation: as a priming signal for ATP-activated macrophages, in determining where the administration of dietary FAs interferes with LPS-mediated inflammasome activation and by inducing inflammasome activation per se. In this study, we confirm that saturated (SFAs) activated the NLRP3 inflammasome and stimulated the secretion of the IL-1β cytokine, while PUFAs were mainly inhibitors. Moreover, in general, DHA (n-3 PUFA) was more effective in preventing inflammasome activation than arachidonic acid (n-6 PUFA).

Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Food science & technology Food science Farmacia Educação física Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biochemistry & molecular biology

licence for use: https://creativecommons.org/licenses/by/3.0/es/

Author's mail: neus.martinez@urv.cat montserrat.pinent@urv.cat mteresa.blay@urv.cat anna.ardevol@urv.cat

Author identifier: 0000-0003-3550-5378 0000-0002-6256-9847 0000-0003-0156-7538

Record's date: 2023-09-09

Papper version: info:eu-repo/semantics/publishedVersion

Link to the original source: https://pubs.rsc.org/en/content/articlelanding/2016/FO/C6FO00477F#!divAbstract

Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/

Papper original source: Food & Function. 7 (8): 3480-3487

APA: Martinez-Micaelo, N.; Gonzalez-Abuin, N.; Pinent, M.; Ardevol, A.; Blay, M.; (2016). Dietary fatty acid composition is sensed by the NLRP3 inflammasome: omega-3 fatty acid (DHA) prevents NLRP3 activation in human macrophages. Food & Function, 7(8), 3480-3487. DOI: 10.1039/C6FO00477F

Article's DOI: 10.1039/c6fo00477f

Entity: Universitat Rovira i Virgili

Journal publication year: 2016

Publication Type: Journal Publications