Articles producció científica> Bioquímica i Biotecnologia

De Novo Synthesis of Benzenoid Compounds by the Yeast Hanseniaspora vineae Increases the Flavor Diversity of Wines

  • Identification data

    Identifier: imarina:5130496
    Authors:
    Martin, ValentinaGiorello, FacundoFarina, LauraMinteguiaga, ManuelSalzman, ValentinaBoido, EduardoAguilar, Pablo S.Gaggero, CarinaDellacassa, EduardoMas, AlbertCarrau, Francisco
    Abstract:
    Benzyl alcohol and other benzenoid-derived metabolites of particular importance in plants confer floral and fruity flavors to wines. Among the volatile aroma components in Vitis vinifera grape varieties, benzyl alcohol is present in its free and glycosylated forms. These compounds are considered to originate from grapes only and not from fermentative processes. We have found increased levels of benzyl alcohol in red Tannat wine compared to that in grape juice, suggesting de novo formation of this metabolite during vinification. In this work, we show that benzyl alcohol, benzaldehyde, p-hydroxybenzaldehyde, and p-hydroxybenzyl alcohol are synthesized de novo in the absence of grape-derived precursors by Hanseniaspora vineae. Levels of benzyl alcohol produced by 11 different H. vineae strains were 20-200 times higher than those measured in fermentations with Saccharomyces cerevisiae strains. These results show that H. vineae contributes to flavor diversity by increasing grape variety aroma concentration in a chemically defined medium. Feeding experiments with phenylalanine, tryptophan, tyrosine, p-aminobenzoic acid, and ammonium in an artificial medium were tested to evaluate the effect of these compounds either as precursors or as potential pathway regulators for the formation of benzenoid-derived aromas. Genomic analysis shows that the phenylalanine ammonia-lyase (PAL) and tyrosine ammonia lyase (TAL) pathways, used by plants to generate benzyl alcohols from aromatic amino acids, are absent in the H. vineae genome. Consequently, alternative pathways derived from chorismate with mandelate as an intermediate are discussed.
  • Others:

    Author, as appears in the article.: Martin, Valentina; Giorello, Facundo; Farina, Laura; Minteguiaga, Manuel; Salzman, Valentina; Boido, Eduardo; Aguilar, Pablo S.; Gaggero, Carina; Dellacassa, Eduardo; Mas, Albert; Carrau, Francisco;
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Mas Baron, Alberto
    Keywords: Wine yeast fermentation Pal/tal alternative biosynthetic pathway Hanseniaspora vineae Genome Benzyl alcohol pal/tal alternative biosynthetic pathway hanseniaspora vineae genome benzyl alcohol
    Abstract: Benzyl alcohol and other benzenoid-derived metabolites of particular importance in plants confer floral and fruity flavors to wines. Among the volatile aroma components in Vitis vinifera grape varieties, benzyl alcohol is present in its free and glycosylated forms. These compounds are considered to originate from grapes only and not from fermentative processes. We have found increased levels of benzyl alcohol in red Tannat wine compared to that in grape juice, suggesting de novo formation of this metabolite during vinification. In this work, we show that benzyl alcohol, benzaldehyde, p-hydroxybenzaldehyde, and p-hydroxybenzyl alcohol are synthesized de novo in the absence of grape-derived precursors by Hanseniaspora vineae. Levels of benzyl alcohol produced by 11 different H. vineae strains were 20-200 times higher than those measured in fermentations with Saccharomyces cerevisiae strains. These results show that H. vineae contributes to flavor diversity by increasing grape variety aroma concentration in a chemically defined medium. Feeding experiments with phenylalanine, tryptophan, tyrosine, p-aminobenzoic acid, and ammonium in an artificial medium were tested to evaluate the effect of these compounds either as precursors or as potential pathway regulators for the formation of benzenoid-derived aromas. Genomic analysis shows that the phenylalanine ammonia-lyase (PAL) and tyrosine ammonia lyase (TAL) pathways, used by plants to generate benzyl alcohols from aromatic amino acids, are absent in the H. vineae genome. Consequently, alternative pathways derived from chorismate with mandelate as an intermediate are discussed.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Planejamento urbano e regional / demografia Odontología Nutrição Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar General medicine General chemistry General agricultural and biological sciences Food science & technology Farmacia Ensino Engenharias iv Engenharias iii Engenharias ii Educação Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Chemistry, applied Chemistry (miscellaneous) Chemistry (all) Biotecnología Biodiversidade Astronomia / física Agriculture, multidisciplinary Agriculture Agricultural and biological sciences (miscellaneous) Agricultural and biological sciences (all)
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: albert.mas@urv.cat
    Author identifier: 0000-0002-0763-1679
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://pubs.acs.org/doi/10.1021/acs.jafc.5b05442
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Journal Of Agricultural And Food Chemistry. 64 (22): 4574-4583
    APA: Martin, Valentina; Giorello, Facundo; Farina, Laura; Minteguiaga, Manuel; Salzman, Valentina; Boido, Eduardo; Aguilar, Pablo S.; Gaggero, Carina; Dell (2016). De Novo Synthesis of Benzenoid Compounds by the Yeast Hanseniaspora vineae Increases the Flavor Diversity of Wines. Journal Of Agricultural And Food Chemistry, 64(22), 4574-4583. DOI: 10.1021/acs.jafc.5b05442
    Article's DOI: 10.1021/acs.jafc.5b05442
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications
  • Keywords:

    Agricultural and Biological Sciences (Miscellaneous),Agriculture,Agriculture, Multidisciplinary,Chemistry (Miscellaneous),Chemistry, Applied,Food Science & Technology
    Wine yeast fermentation
    Pal/tal alternative biosynthetic pathway
    Hanseniaspora vineae
    Genome
    Benzyl alcohol
    pal/tal alternative biosynthetic pathway
    hanseniaspora vineae
    genome
    benzyl alcohol
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Planejamento urbano e regional / demografia
    Odontología
    Nutrição
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Interdisciplinar
    General medicine
    General chemistry
    General agricultural and biological sciences
    Food science & technology
    Farmacia
    Ensino
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Educação
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Chemistry, applied
    Chemistry (miscellaneous)
    Chemistry (all)
    Biotecnología
    Biodiversidade
    Astronomia / física
    Agriculture, multidisciplinary
    Agriculture
    Agricultural and biological sciences (miscellaneous)
    Agricultural and biological sciences (all)
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