Author, as appears in the article.: Gil-Cardoso, K; Gines, I; Pinent, M; Ardevol, A; Terra, X; Blay, M
Department: Enginyeria Química Bioquímica i Biotecnologia
URV's Author/s: Ardévol Grau, Anna / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Keywords: Zonula occludens-1 protein Zo zonula occludens Weight gain Tumor necrosis factor-alpha Tj tight junctions Rv reverse Ros reactive oxygen species Receptors, leptin Reactive oxygen species Rats, zucker Rats, wistar Proanthocyanidins Peroxidase Pc principal components Oxidative stress Ocln occludin Occludin Obesity Mpo myeloperoxidase Lepr leptin receptor Intestine Inos isoform of nitric oxide synthase Inflammation Ileum Homa-ir homeostasis model assessment of insulin resistance Gut microbiota Gut Go genetic obesity Gi gastrointestinal Fw forward Female Feeding behavior Diet Claudin-1 Cafeteria diet Caf diet cafeteria diet Animals obesity inflammation gut cafeteria diet zo zonula occludens tj tight junctions rv reverse ros reactive oxygen species pc principal components ocln occludin mpo myeloperoxidase lepr leptin receptor inos isoform of nitric oxide synthase homa-ir homeostasis model assessment of insulin resistance go genetic obesity gi gastrointestinal fw forward caf diet cafeteria diet
Abstract: The gastrointestinal alterations associated with the consumption of an obesogenic diet, such as inflammation, permeability impairment and oxidative stress, have been poorly explored in both diet-induced obesity (DIO) and genetic obesity. The aim of the present study was to examine the impact of an obesogenic diet on the gut health status of DIO rats in comparison with the Zucker (fa/fa) rat leptin receptor-deficient model of genetic obesity over time. For this purpose, female Wistar rats (n 48) were administered a standard or a cafeteria diet (CAF diet) for 12, 14·5 or 17 weeks and were compared with fa/fa Zucker rats fed a standard diet for 10 weeks. Morphometric variables, plasma biochemical parameters, myeloperoxidase (MPO) activity and reactive oxygen species (ROS) levels in the ileum were assessed, as well as the expressions of proinflammatory genes (TNF-α and inducible nitric oxide synthase (iNOS)) and intestinal permeability genes (zonula occludens-1, claudin-1 and occludin). Both the nutritional model and the genetic obesity model showed increased body weight and metabolic alterations at the final time point. An increase in intestinal ROS production and MPO activity was observed in the gastrointestinal tracts of rats fed a CAF diet but not in the genetic obesity model. TNF-α was overexpressed in the ileum of both CAF diet and fa/fa groups, and ileal inflammation was associated with the degree of obesity and metabolic alterations. Interestingly, the 17-week CAF group and the fa/fa rats exhibited alterations in the expressions of permeability genes. Relevantly, in the hyperlipidic refined sugar diet model of obesity, the responses to chronic energy overload led to time-dependent increases in gut inflammation and oxidative stress.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrition and dietetics Nutrition & dietetics Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Matemática / probabilidade e estatística Interdisciplinar General medicine Farmacia Engenharias iv Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Administração pública e de empresas, ciências contábeis e turismo
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 14752662
Author's mail: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat
Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378
Record's date: 2024-10-19
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.cambridge.org/core/journals/british-journal-of-nutrition/article/cafeteria-diet-triggers-intestinal-inflammation-and-oxidative-stress-in-obese-rats/36481830A5146F496471C39333F94D57
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: British Journal Of Nutrition. 117 (2): 218-229
APA: Gil-Cardoso, K; Gines, I; Pinent, M; Ardevol, A; Terra, X; Blay, M (2017). A cafeteria diet triggers intestinal inflammation and oxidative stress in obese rats. British Journal Of Nutrition, 117(2), 218-229. DOI: 10.1017/S0007114516004608
Article's DOI: 10.1017/S0007114516004608
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications