Articles producció científica> Ciències Mèdiques Bàsiques

Daylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii

  • Identification data

    Identifier: imarina:5130934
    Authors:
    Perez-Laguna, VanesaRezusta, AntonioJose Ramos, JuanMiguel Ferrer, LuisGene, JosepaJose Revillo, MariaGilaberte, Yolanda
    Abstract:
    Arthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.
  • Others:

    Author, as appears in the article.: Perez-Laguna, Vanesa; Rezusta, Antonio; Jose Ramos, Juan; Miguel Ferrer, Luis; Gene, Josepa; Jose Revillo, Maria; Gilaberte, Yolanda;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Gené Díaz, Josepa
    Keywords: Trichophyton spp. Tinea/ringworm Photoinactivation Photodynamic therapy
    Abstract: Arthroderma vanbreuseghemii has been identified molecularly as the causative agent of dermatophytosis in a flock of sheep. It is necessary to explore new treatment alternatives because antifungals are not approved for use on small ruminant animals in the European Union. Antimicrobial photodynamic therapy (aPDT) has been shown to be effective for the treatment of dermatophytosis in humans. It is based on the application of a photosensitizer such as methylene blue (MB) that is activated by visible light to generate reactive oxygen species that are cytotoxic to cells. The use of daylight to perform aPDT (aDL-PDT) avoids the requirement of specific equipment because it uses sunlight to activate the photosensitizer. The aim of our study is to determine the efficacy of aDL-PDT using a 1% MB solution to treat dermatophytosis caused by A. vanbreuseghemii in ewes. Two different topical protocols (1% MB solution spray applications once or twice a week) were assayed in two groups of five infected animals. Twenty-five infected sheep were untreated. All the sheep were exposed to sunlight every day for an approximate duration of 10 h for a total of four weeks. At the end of the study, all the animals treated with aDL-PDT showed the same clinical response to both protocols. In contrast, the animals exposed only to sunlight required an additional two to four weeks before their infections resolved. Conclusion: aDL-PDT with 1% MB solution demonstrates efficacy, safety and efficiency in the treatment of dermatophytosis in sheep.
    Thematic Areas: Zootecnia / recursos pesqueiros Química Nutrição Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geografía Food animals Farmacia Engenharias iv Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade Animal science and zoology Agriculture, dairy & animal science
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: josepa.gene@urv.cat
    Author identifier: 0000-0001-6195-9299
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0921448817300792?via%3Dihub
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Small Ruminant Research. 150 97-101
    APA: Perez-Laguna, Vanesa; Rezusta, Antonio; Jose Ramos, Juan; Miguel Ferrer, Luis; Gene, Josepa; Jose Revillo, Maria; Gilaberte, Yolanda; (2017). Daylight photodynamic therapy using methylene blue to treat sheep with dermatophytosis caused by Arthroderma vanbreuseghemii. Small Ruminant Research, 150(), 97-101. DOI: 10.1016/j.smallrumres.2017.03.011
    Article's DOI: 10.1016/j.smallrumres.2017.03.011
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications
  • Keywords:

    Agriculture, Dairy & Animal Science,Animal Science and Zoology,Food Animals
    Trichophyton spp.
    Tinea/ringworm
    Photoinactivation
    Photodynamic therapy
    Zootecnia / recursos pesqueiros
    Química
    Nutrição
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Geografía
    Food animals
    Farmacia
    Engenharias iv
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
    Animal science and zoology
    Agriculture, dairy & animal science
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