Articles producció científica> Ciències Mèdiques Bàsiques

Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts

  • Identification data

    Identifier: imarina:5131119
    Authors:
    López-Díaz C, Rahjoo V, Sulyok M, Ghionna V, Martín-Vicente A, Capilla J, Di Pietro A, López-Berges MS.
    Abstract:
    Fusaric acid (FA) is amongst the oldest identified secondary metabolites produced by Fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of FA and its function in fungal pathogenicity remain unknown. Here, we investigated the role of FA in Fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. Targeted deletion of fub1, encoding a predicted orthologue of the polyketide synthase involved in FA biosynthesis in F. verticillioides and F. fujikuroi, abolished the production of FA and its derivatives in F. oxysporum. We further showed that the expression of fub1 was positively controlled by the master regulator of secondary metabolism LaeA and the alkaline pH regulator PacC through the modulation of chromatin accessibility at the fub1 locus. FA exhibited strong phytotoxicity on tomato plants, which was rescued by the exogenous supply of copper, iron or zinc, suggesting a possible function of FA as a chelating agent of these metal ions. Importantly, the severity of vascular wilt symptoms on tomato plants and the mortality of immunosuppressed mice were significantly reduced in fub1Δ mutants and fully restored in the complemented strains. Collectively, these results provide new insights into the regulation and mode of action of FA, as well as on the function of this phytotoxin during the infection process of F. oxysporum.
  • Others:

    Author, as appears in the article.: López-Díaz C, Rahjoo V, Sulyok M, Ghionna V, Martín-Vicente A, Capilla J, Di Pietro A, López-Berges MS.
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Capilla Luque, Javier
    Keywords: Virulence Phytotoxicity Mycotoxins Metal chelation Fusarium oxysporum Fusaric acid Fungal pathogenicity phytotoxicity mycotoxins metal chelation fusarium oxysporum fusaric acid fungal pathogenicity
    Abstract: Fusaric acid (FA) is amongst the oldest identified secondary metabolites produced by Fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of FA and its function in fungal pathogenicity remain unknown. Here, we investigated the role of FA in Fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. Targeted deletion of fub1, encoding a predicted orthologue of the polyketide synthase involved in FA biosynthesis in F. verticillioides and F. fujikuroi, abolished the production of FA and its derivatives in F. oxysporum. We further showed that the expression of fub1 was positively controlled by the master regulator of secondary metabolism LaeA and the alkaline pH regulator PacC through the modulation of chromatin accessibility at the fub1 locus. FA exhibited strong phytotoxicity on tomato plants, which was rescued by the exogenous supply of copper, iron or zinc, suggesting a possible function of FA as a chelating agent of these metal ions. Importantly, the severity of vascular wilt symptoms on tomato plants and the mortality of immunosuppressed mice were significantly reduced in fub1Δ mutants and fully restored in the complemented strains. Collectively, these results provide new insights into the regulation and mode of action of FA, as well as on the function of this phytotoxin during the infection process of F. oxysporum.
    Thematic Areas: Soil science Química Plant sciences Plant science Molecular biology Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Agronomy and crop science
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 14646722
    Author's mail: javier.capilla@urv.cat
    Author identifier: 0000-0002-0765-6403
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.12536
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Molecular Plant Pathology. 19 (2): 440-453
    APA: López-Díaz C, Rahjoo V, Sulyok M, Ghionna V, Martín-Vicente A, Capilla J, Di Pietro A, López-Berges MS. (2018). Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts. Molecular Plant Pathology, 19(2), 440-453. DOI: 10.1111/mpp.12536
    Article's DOI: 10.1111/mpp.12536
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    Publication Type: Journal Publications
  • Keywords:

    Agronomy and Crop Science,Molecular Biology,Plant Science,Plant Sciences,Soil Science
    Virulence
    Phytotoxicity
    Mycotoxins
    Metal chelation
    Fusarium oxysporum
    Fusaric acid
    Fungal pathogenicity
    phytotoxicity
    mycotoxins
    metal chelation
    fusarium oxysporum
    fusaric acid
    fungal pathogenicity
    Soil science
    Química
    Plant sciences
    Plant science
    Molecular biology
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Biotecnología
    Biodiversidade
    Agronomy and crop science
  • Documents:

  • Cerca a google

    Search to google scholar