Author, as appears in the article.: López-Díaz C, Rahjoo V, Sulyok M, Ghionna V, Martín-Vicente A, Capilla J, Di Pietro A, López-Berges MS.
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Capilla Luque, Javier
Keywords: Virulence Phytotoxicity Mycotoxins Metal chelation Fusarium oxysporum Fusaric acid Fungal pathogenicity phytotoxicity mycotoxins metal chelation fusarium oxysporum fusaric acid fungal pathogenicity
Abstract: Fusaric acid (FA) is amongst the oldest identified secondary metabolites produced by Fusarium species, known for a long time to display strong phytotoxicity and moderate toxicity to animal cells; however, the cellular targets of FA and its function in fungal pathogenicity remain unknown. Here, we investigated the role of FA in Fusarium oxysporum, a soil-borne cross-kingdom pathogen that causes vascular wilt on more than 100 plant species and opportunistic infections in humans. Targeted deletion of fub1, encoding a predicted orthologue of the polyketide synthase involved in FA biosynthesis in F. verticillioides and F. fujikuroi, abolished the production of FA and its derivatives in F. oxysporum. We further showed that the expression of fub1 was positively controlled by the master regulator of secondary metabolism LaeA and the alkaline pH regulator PacC through the modulation of chromatin accessibility at the fub1 locus. FA exhibited strong phytotoxicity on tomato plants, which was rescued by the exogenous supply of copper, iron or zinc, suggesting a possible function of FA as a chelating agent of these metal ions. Importantly, the severity of vascular wilt symptoms on tomato plants and the mortality of immunosuppressed mice were significantly reduced in fub1Δ mutants and fully restored in the complemented strains. Collectively, these results provide new insights into the regulation and mode of action of FA, as well as on the function of this phytotoxin during the infection process of F. oxysporum.
Thematic Areas: Soil science Química Plant sciences Plant science Molecular biology Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Agronomy and crop science
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 14646722
Author's mail: javier.capilla@urv.cat
Author identifier: 0000-0002-0765-6403
Record's date: 2023-02-18
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://bsppjournals.onlinelibrary.wiley.com/doi/full/10.1111/mpp.12536
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Molecular Plant Pathology. 19 (2): 440-453
APA: López-Díaz C, Rahjoo V, Sulyok M, Ghionna V, Martín-Vicente A, Capilla J, Di Pietro A, López-Berges MS. (2018). Fusaric acid contributes to virulence of Fusarium oxysporum on plant and mammalian hosts. Molecular Plant Pathology, 19(2), 440-453. DOI: 10.1111/mpp.12536
Article's DOI: 10.1111/mpp.12536
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV