Author, as appears in the article.: H Julia Ellis, M Carmen Bermudo Redondo, Tanja Suligoj, Beatriz Corte-Real , Paul J Ciclitira
Department: Enginyeria Química
URV's Author/s: Bermudo Redondo, Maria del Carmen
Keywords: Mostres reals Immunoassaig Gluten Gliadin Detecció del gluten
Abstract: Coeliac disease (CD) is an immune mediated condition involving dietary gluten, found in wheat, rye, and barley; avoiding them is the only treatment. We generated a mAb to the immunodominant gliadin epitope alpha-2-gli-57-75 and developed assays to quantify gluten in foods, comparing the results with those from the R5 assay. Competition assays were developed using native gliadin as the competing and coating antigen. Results were compared with those from the Ingenesa Ingezim Gluten ® kit. The mAb recognized prolamins from wheat, spelt, rye and barley. A competition assay that detected gliadin at 0.027ppm in a foodstuff revealed gliadin contamination of several foods labelled gluten-free. Purified wheat starch, used as wheat flour substitute to generate gluten-free foods, contains quantifiable amounts of the immunodominant epitope. The assay is sufficiently sensitive to assess the suitability of foods for coeliac patients and could be used as an alternative to the R5.
Thematic Areas: Saúde coletiva Nutrição Educação física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: carmen.bermudo@urv.cat carmen.bermudo@urv.cat carmen.bermudo@urv.cat
Author identifier: 0000-0002-4128-9803 0000-0002-4128-9803 0000-0002-4128-9803
Record's date: 2024-01-13
Papper version: info:eu-repo/semantics/publishedVersion
Papper original source: Food And Nutrition Report. 1 (3): 1-7
APA: H Julia Ellis, M Carmen Bermudo Redondo, Tanja Suligoj, Beatriz Corte-Real , Paul J Ciclitira (2016). Gluten Quantification of foods via the Immunodominang Gliadin Epitope. Food And Nutrition Report, 1(3), 1-7. DOI: 10.24218/fnr.2015.11
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV