Author, as appears in the article.: Pachon-Pena, Gisela; Donnelly, Conor; Ruiz-Canada, Catalina; Katz, Adam; Fernandez-Veledo, Sonia; Vendrell, Joan; Sackstein, Robert;
Department: Medicina i Cirurgia Ciències Mèdiques Bàsiques
URV's Author/s: Fernandez Veledo, Sonia / Vendrell Ortega, Juan José
Keywords: Stromal cells Slex Sle(x) Sle x Sialyl lewis x Mesenchymal stem cell Hematopoietic cell e-/l-selectin ligand Hematopoietic cell e-/ l-selectin ligand Glycosyltransferase-programmed stereosubstitution Fucosyltransferase Fucosyl-transferases Exofucosylation E-selectin ligands E-selectin ligand sialyl lewis x mesenchymal stem cell hematopoietic cell e-/l-selectin ligand glycosyltransferase-programmed stereosubstitution fucosyltransferase exofucosylation e-selectin ligand
Abstract: The clinical effectiveness of systemically administered human mesenchymal stem cells (hMSCs) depends on their capacity to engage vascular endothelium. hMSCs derived from bone marrow (BM-hMSCs) natively lack endothelial binding capacity, but express a CD44 glycovariant containing N-linked sialyllactosamines that can be α(1,3)-fucosylated using fucosyltransferase-VI (FTVI) to enforce sLeX decorations, thereby creating hematopoietic cell E-/L-selectin ligand (HCELL). HCELL expression programs potent shear-resistant adhesion of circulating cells to endothelial beds expressing E-selectin. An alternative source of hMSCs is adipose tissue (A-hMSCs), and we assessed whether A-hMSCs bind E-selectin and/or possess sialyllactosamine-decorated CD44 accessible to α(1,3)-fucosylation. Similar to BM-hMSCs, we found that A-hMSCs natively lack E-selectin ligands, but FTVI-mediated cell surface α(1,3)-fucosylation induces sLeX expression and robust E-selectin binding secondary to conversion of CD44 into HCELL. Moreover, treatment with the α(1,3)-fucosyltransferase-FTVII also generated expression of HCELL on both BM-hMSCs and A-hMSCs, with sLeX decorations created on N-linked glycans of the 'standard' CD44 (CD44s) isoform. The finding that hMSCs from both source tissues each lack native E-selectin ligand expression prompted examination of the expression of glycosyltransferases that direct lactosaminyl glycan synthesis. These studies reveal that both types of hMSCs conspicuously lack transcripts encoding α(1,3)-fucosyltransferases, but equally express glycosyltransferases critical to creation of sialyllactosamines. Collectively, these data indicate that assembly of a sialyllactosaminyl-decorated CD44s glycovariant is a conserved feature of hMSCs derived from adipose tissue and marrow, thus identifying a CD44 glycosignature of these cells and supporting the applicability of cell surface α(1,3)-fucosylation in programming migration of systemically administered A-hMSCs to sites of tissue injury/inflammation. Stem Cells 2017;35:1080-1092.
Thematic Areas: Oncology Nutrição Molecular medicine Medicine (miscellaneous) Medicine (all) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Hematology General medicine Farmacia Engenharias ii Educação física Developmental biology Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Cell & tissue engineering Biotecnología Biotechnology & applied microbiology
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 15494918
Author's mail: sonia.fernandez@urv.cat juanjose.vendrell@urv.cat
Author identifier: 0000-0003-2906-3788 0000-0002-6994-6115
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Stem Cells. 35 (4): 1080-1092
APA: Pachon-Pena, Gisela; Donnelly, Conor; Ruiz-Canada, Catalina; Katz, Adam; Fernandez-Veledo, Sonia; Vendrell, Joan; Sackstein, Robert; (2017). A Glycovariant of Human CD44 Is Characteristically Expressed on Human Mesenchymal Stem Cells. Stem Cells, 35(4), 1080-1092. DOI: 10.1002/stem.2549
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications
Repositori URV