Articles producció científica> Enginyeria Química

Apatite nanoparticles strongly improve red blood cell cryopreservation by mediating trehalose delivery via enhanced membrane permeation

  • Identification data

    Identifier: imarina:5131502
  • Authors:

    Stefanic, Martin
    Ward, Kevin
    Tawfik, Harvey
    Seemann, Ralf
    Baulin, Vladimir
    Guo, Yachong
    Fleury, Jean-Baptiste
    Drouet, Christophe
  • Others:

    Author, as appears in the article.: Stefanic, Martin; Ward, Kevin; Tawfik, Harvey; Seemann, Ralf; Baulin, Vladimir; Guo, Yachong; Fleury, Jean-Baptiste; Drouet, Christophe;
    Department: Enginyeria Química
    URV's Author/s: Baulin, Vladimir / GUO, YACHONG
    Keywords: Suspensions Red blood cells Preservation Polymers Nanoparticles Nanomedicine Membrane permeation Lipid-bilayers Lipid bilayer Cryosurvival Cryopreservation Colloids Biomimetic apatite Biomedical applications Apatite Adsorption nanoparticles membrane permeation lipid bilayer cryopreservation apatite
    Abstract: Cryopreservation of red blood cells (RBC) is an important method for maintaining an inventory of rare RBC units and managing special transfusion circumstances. Currently, in a clinical setting, glycerol is used as cryoprotectant against freezing damage. After thawing and before transfusion, glycerol must however be removed to avoid intravascular hemolysis, via a complex and time-consuming deglycerolization process which requires specialized equipment. Improved cryopreservation methods using non-toxic agents are required to increase biocompatibility and decrease processing time. Biocompatible cryoprotectants (e.g. trehalose) were proposed, but their low permeation through RBC membranes limits their cryoprotection efficacy. Herein, we report for the first time a glycerol-free cryopreservation approach, using colloidal bioinspired apatite nanoparticles (NP) as bioactive promoters of RBC cryopreservation mediated by trehalose. Addition of apatite NP in the medium tremendously increases RBC cryosurvival, up to 91% (42% improvement compared to a control without NP) which is comparable to FDA-approved cryoprotection protocol employing glycerol. NP concentration and incubation conditions strongly modulate the NP bioactivity. Complementary experimental and computational analyses of the interaction between apatite NP and model lipid bilayers revealed complex events occurring at the NP-bilayer interface. Apatite NP do not cross the bilayer but momentarily modulate its physical status. These changes affect the membrane behavior, and promote the permeation of trehalose and a model fluorescent molecule (FITC). This approach is a new alternative to using toxic glycerol for cells cryopreservation, and the identification of this enhancing no-pore permeation mechanism of apatite NP appears as an original delivery pathway for cryoprotectant agents and beyond.
    Thematic Areas: Química Odontología Nanoscience and nanotechnology Medicina veterinaria Medicina ii Mechanics of materials Materials science, biomaterials Interdisciplinar General medicine Farmacia Engineering, biomedical Engenharias iv Engenharias ii Ciências biológicas ii Ciências biológicas i Ceramics and composites Biotecnología Biophysics Biomaterials Bioengineering Biodiversidade
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: vladimir.baulin@urv.cat
    Author identifier: 0000-0003-2086-4271
    Record's date: 2023-05-20
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0142961217304179
    Papper original source: Biomaterials. 140 138-149
    APA: Stefanic, Martin; Ward, Kevin; Tawfik, Harvey; Seemann, Ralf; Baulin, Vladimir; Guo, Yachong; Fleury, Jean-Baptiste; Drouet, Christophe; (2017). Apatite nanoparticles strongly improve red blood cell cryopreservation by mediating trehalose delivery via enhanced membrane permeation. Biomaterials, 140(), 138-149. DOI: 10.1016/j.biomaterials.2017.06.018
    Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.1016/j.biomaterials.2017.06.018
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2017
    Publication Type: Journal Publications
  • Keywords:

    Bioengineering,Biomaterials,Biophysics,Ceramics and Composites,Engineering, Biomedical,Materials Science, Biomaterials,Mechanics of Materials,Nanoscience and Nanotechnology
    Suspensions
    Red blood cells
    Preservation
    Polymers
    Nanoparticles
    Nanomedicine
    Membrane permeation
    Lipid-bilayers
    Lipid bilayer
    Cryosurvival
    Cryopreservation
    Colloids
    Biomimetic apatite
    Biomedical applications
    Apatite
    Adsorption
    nanoparticles
    membrane permeation
    lipid bilayer
    cryopreservation
    apatite
    Química
    Odontología
    Nanoscience and nanotechnology
    Medicina veterinaria
    Medicina ii
    Mechanics of materials
    Materials science, biomaterials
    Interdisciplinar
    General medicine
    Farmacia
    Engineering, biomedical
    Engenharias iv
    Engenharias ii
    Ciências biológicas ii
    Ciências biológicas i
    Ceramics and composites
    Biotecnología
    Biophysics
    Biomaterials
    Bioengineering
    Biodiversidade
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