Author, as appears in the article.: Martínez-Micaelo, N; Beltrán-Debón, R; Aragonés, G; Faiges, M; Alegret, JM
Department: Bioquímica i Biotecnologia
URV's Author/s: Alegret Colomé, Josep Maria / Aragonès Bargalló, Gerard / Beltrán Debón, Raúl Alejandro
Keywords: Microrna Endothelial damage Co-expression network Circulating endothelial microparticles Bioinformatics Bicuspid aortic valve Aortic dilation endothelial damage co-expression network circulating endothelial microparticles bioinformatics bicuspid aortic valve aortic dilation
Abstract: Background: We previously described that PECAM+ circulating endothelial microparticles (EMPs) are elevated in bicuspid aortic valve (BAV) disease as a manifestation of endothelial damage. In this study, we hypothesized that this endothelial damage, is functionally related to the secretion of a specific pattern of EMP-associated miRNAs. Methods: We used a bioinformatics approach to correlate the PECAM+ EMP levels with the miRNA expression profile in plasma in healthy individuals and BAV patients (n = 36). In addition, using the miRNAs that were significantly associated with PECAM+ EMP levels, we inferred a miRNA co-expression network using a Gaussian graphical modeling approach to identify highly co-expressed miRNAs or miRNA clusters whose expression could functionally regulate endothelial damage. Results: We identified a co-expression network composed of 131 miRNAs whose circulating expression was significantly associated with PECAM+ EMP levels. Using a topological analysis, we found that miR-494 was the most important hub within the co-expression network. Furthermore, through positional gene enrichment analysis, we identified a cluster of 19 highly co-expressed miRNAs, including miR-494, that was located in the 14q32 locus on chromosome 14 (p = 1.9 × 10-7). We evaluated the putative biological role of this miRNA cluster by determining the biological significance of the genes targeted by the cluster using functional enrichment analysis. We found that this cluster was involved in the regulation of genes with various functions, specifically the 'cellular nitrogen compound metabolic process' (p = 2.34 × 10-145), 'immune system process' (p = 2.57 × 10-6), and 'extracellular matrix organization' (p = 8.14 × 10-5) gene ontology terms and the 'TGF-β signaling pathway' KEGG term (p = 2.59 × 10-8). Conclusions: Using an integrative bioinformatics approach, we identified the circulating miRNA expression profile associated with secreted PECAM+ EMPs in BAV disease. Additionally, we identified a highly co-expressed miRNA cluster that could mediate crucial biological processes in BAV disease, including the nitrogen signaling pathway, cellular activation, and the transforming growth factor beta signaling pathway. In conclusion, EMP-associated and co-expressed miRNAs could act as molecular effectors of the intercellular communication carried out by EMPs in response to endothelial damage.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Physiology (medical) Physiology Odontología Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Farmacia Ensino Engenharias iv Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Astronomia / física Administração pública e de empresas, ciências contábeis e turismo
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 1664042X
Author's mail: josepmaria.alegret@urv.cat gerard.aragones@urv.cat raul.beltran@urv.cat
Author identifier: 0000-0002-6117-5512 0000-0001-9691-1906
Record's date: 2024-07-27
Papper version: info:eu-repo/semantics/publishedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Frontiers In Physiology. 8 (SEP): 648-
APA: Martínez-Micaelo, N; Beltrán-Debón, R; Aragonés, G; Faiges, M; Alegret, JM (2017). MicroRNAs Clustered within the 14q32 Locus Are Associated with Endothelial Damage and Microparticle Secretion in Bicuspid Aortic Valve Disease.. Frontiers In Physiology, 8(SEP), 648-. DOI: 10.3389/fphys.2017.00648
Entity: Universitat Rovira i Virgili
Journal publication year: 2017
Publication Type: Journal Publications
Repositori URV