Articles producció científica> Enginyeria Química

The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells

  • Identification data

    Identifier: imarina:5131851
    Authors:
    Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP
    Abstract:
    Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.
  • Others:

    Author, as appears in the article.: Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP
    Department: Enginyeria Química
    URV's Author/s: Baulin, Vladimir
    Keywords: Substratum Proliferation Poly-l-lysine Pc12 neuronal differentiation Neuronal differentiation Nerve growth factor (ngf) Laminin Fibronectin Adhesion pc12 neuronal differentiation nerve growth factor (ngf) laminin fibronectin
    Abstract: Cellular attachment plays a vital role in the differentiation of pheochromocytoma (PC12) cells. PC12 cells are noradrenergic clonal cells isolated from the adrenal medulla of Rattus norvegicus and studied extensively as they have the ability to differentiate into sympathetic neuron-like cells. The effect of several experimental parameters including (i) the concentration of nerve growth factor (NGF); (ii) substratum coatings, such as poly-L-lysine (PLL), fibronectin (Fn), and laminin (Lam); and (iii) double coatings composed of PLL/Lam and PLL/Fn on the differentiation process of PC12 cells were studied. Cell morphology was visualised using brightfield phase contrast microscopy, cellular metabolism and proliferation were quantified using a 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay, and the neurite outgrowth and axonal generation of the PC12 cells were evaluated using wide field fluorescence microscopy. It was found that double coatings of PLL/Lam and PLL/Fn supported robust adhesion and a two-fold enhanced neurite outgrowth of PC12 cells when treated with 100 ng/mL of NGF while exhibiting stable metabolic activity, leading to the accelerated generation of axons.
    Thematic Areas: Química Odontología Medicina veterinaria Medicina i Materials science, multidisciplinary Materiais Interdisciplinar General materials science Engenharias iv Engenharias iii Engenharias ii Engenharias i Condensed matter physics Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Astronomia / física Arquitetura, urbanismo e design
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 19961944
    Author's mail: vladimir.baulin@urv.cat
    Author identifier: 0000-0003-2086-4271
    Record's date: 2023-07-31
    Journal volume: 11
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1996-1944/11/1/60
    Papper original source: Materials. 11 (1): 60-
    APA: Orlowska A, Perera PT, Al Kobaisi MA, Dias A, Nguyen HKD, Ghanaati S, Baulin V, Crawford RJ, Ivanova EP (2018). The Effect of Coatings and Nerve Growth Factor on Attachment and Differentiation of Pheochromocytoma Cells. Materials, 11(1), 60-. DOI: 10.3390/ma11010060
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.3390/ma11010060
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    First page: Article number 60
    Publication Type: Journal Publications
  • Keywords:

    Materials Science, Multidisciplinary
    Substratum
    Proliferation
    Poly-l-lysine
    Pc12 neuronal differentiation
    Neuronal differentiation
    Nerve growth factor (ngf)
    Laminin
    Fibronectin
    Adhesion
    pc12 neuronal differentiation
    nerve growth factor (ngf)
    laminin
    fibronectin
    Química
    Odontología
    Medicina veterinaria
    Medicina i
    Materials science, multidisciplinary
    Materiais
    Interdisciplinar
    General materials science
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Condensed matter physics
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Astronomia / física
    Arquitetura, urbanismo e design
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