Articles producció científica> Enginyeria Química

Direct proof of spontaneous translocation of lipid-covered hydrophobic nanoparticles through a phospholipid bilayer

  • Identification data

    Identifier: imarina:5131910
    Authors:
    Guo, YachongTerazzi, EmmanuelSeemann, RalfFleury, Jean BaptisteBaulin, Vladimir A.
    Abstract:
    Hydrophobic nanoparticles introduced into living systems may lead to increased toxicity, can activate immune cells, or can be used as nanocarriers for drug or gene delivery. It is generally accepted that small hydrophobic nanoparticles are blocked by lipid bilayers and accumulate in the bilayer core, whereas big nanoparticles can only penetrate cells through slow energy-dependent processes, such as endocytosis, lasting minutes. In contrast to expectations, we demonstrate that lipid-covered hydrophobic nanoparticles may translocate through lipid membranes by direct penetration within milliseconds. We identified the threshold size for translocation: nanoparticles with diameters smaller than 5 nm stay trapped in the bilayer, whereas those with diameters larger than 5 nm insert into the bilayer, opening pores in the bilayer. The direct proof of this size-dependent translocation was provided by an in situ observation of a single event of a nanoparticle quitting the bilayer. This was achieved with a specially designed microfluidic device combining optical fluorescence microscopy with simultaneous electrophysiological measurements. A quantitative analysis of the kinetic pathway of a single nanoparticle translocation event demonstrated that the translocation is irreversible and that the nanoparticle can translocate only once. This newly discovered one-way translocation mechanism provides numerous opportunities for biotechnological applications, ranging from targeted biomaterial elimination and/or delivery to precise and controlled trapping of nanoparticles.
  • Others:

    Author, as appears in the article.: Guo, Yachong; Terazzi, Emmanuel; Seemann, Ralf; Fleury, Jean Baptiste; Baulin, Vladimir A.;
    Department: Enginyeria Química
    URV's Author/s: Baulin, Vladimir / GUO, YACHONG
    Keywords: Translocation dynamics Toxicity Thermodynamics Penetration Membranes Mechanisms Insertion Gold nanoparticles Disruption Cytotoxicity Cellular uptake nanoparticles microfluidics lipid bilayers
    Abstract: Hydrophobic nanoparticles introduced into living systems may lead to increased toxicity, can activate immune cells, or can be used as nanocarriers for drug or gene delivery. It is generally accepted that small hydrophobic nanoparticles are blocked by lipid bilayers and accumulate in the bilayer core, whereas big nanoparticles can only penetrate cells through slow energy-dependent processes, such as endocytosis, lasting minutes. In contrast to expectations, we demonstrate that lipid-covered hydrophobic nanoparticles may translocate through lipid membranes by direct penetration within milliseconds. We identified the threshold size for translocation: nanoparticles with diameters smaller than 5 nm stay trapped in the bilayer, whereas those with diameters larger than 5 nm insert into the bilayer, opening pores in the bilayer. The direct proof of this size-dependent translocation was provided by an in situ observation of a single event of a nanoparticle quitting the bilayer. This was achieved with a specially designed microfluidic device combining optical fluorescence microscopy with simultaneous electrophysiological measurements. A quantitative analysis of the kinetic pathway of a single nanoparticle translocation event demonstrated that the translocation is irreversible and that the nanoparticle can translocate only once. This newly discovered one-way translocation mechanism provides numerous opportunities for biotechnological applications, ranging from targeted biomaterial elimination and/or delivery to precise and controlled trapping of nanoparticles.
    Thematic Areas: Química Multidisciplinary sciences Multidisciplinary Medicine (miscellaneous) Interdisciplinar Geociências General medicine Engenharias iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: vladimir.baulin@urv.cat
    Author identifier: 0000-0003-2086-4271
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.science.org/doi/10.1126/sciadv.1600261
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Science Advances. 2 (11): e1600261-
    APA: Guo, Yachong; Terazzi, Emmanuel; Seemann, Ralf; Fleury, Jean Baptiste; Baulin, Vladimir A.; (2016). Direct proof of spontaneous translocation of lipid-covered hydrophobic nanoparticles through a phospholipid bilayer. Science Advances, 2(11), e1600261-. DOI: 10.1126/sciadv.1600261
    Article's DOI: 10.1126/sciadv.1600261
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2016
    Publication Type: Journal Publications
  • Keywords:

    Medicine (Miscellaneous),Multidisciplinary,Multidisciplinary Sciences
    Translocation dynamics
    Toxicity
    Thermodynamics
    Penetration
    Membranes
    Mechanisms
    Insertion
    Gold nanoparticles
    Disruption
    Cytotoxicity
    Cellular uptake
    nanoparticles
    microfluidics
    lipid bilayers
    Química
    Multidisciplinary sciences
    Multidisciplinary
    Medicine (miscellaneous)
    Interdisciplinar
    Geociências
    General medicine
    Engenharias iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Biotecnología
    Biodiversidade
    Astronomia / física
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