Author, as appears in the article.: Guo, Yachong; Terazzi, Emmanuel; Seemann, Ralf; Fleury, Jean Baptiste; Baulin, Vladimir A.;
Department: Enginyeria Química
URV's Author/s: Baulin, Vladimir / GUO, YACHONG
Keywords: Translocation dynamics Toxicity Thermodynamics Penetration Membranes Mechanisms Insertion Gold nanoparticles Disruption Cytotoxicity Cellular uptake nanoparticles microfluidics lipid bilayers
Abstract: Hydrophobic nanoparticles introduced into living systems may lead to increased toxicity, can activate immune cells, or can be used as nanocarriers for drug or gene delivery. It is generally accepted that small hydrophobic nanoparticles are blocked by lipid bilayers and accumulate in the bilayer core, whereas big nanoparticles can only penetrate cells through slow energy-dependent processes, such as endocytosis, lasting minutes. In contrast to expectations, we demonstrate that lipid-covered hydrophobic nanoparticles may translocate through lipid membranes by direct penetration within milliseconds. We identified the threshold size for translocation: nanoparticles with diameters smaller than 5 nm stay trapped in the bilayer, whereas those with diameters larger than 5 nm insert into the bilayer, opening pores in the bilayer. The direct proof of this size-dependent translocation was provided by an in situ observation of a single event of a nanoparticle quitting the bilayer. This was achieved with a specially designed microfluidic device combining optical fluorescence microscopy with simultaneous electrophysiological measurements. A quantitative analysis of the kinetic pathway of a single nanoparticle translocation event demonstrated that the translocation is irreversible and that the nanoparticle can translocate only once. This newly discovered one-way translocation mechanism provides numerous opportunities for biotechnological applications, ranging from targeted biomaterial elimination and/or delivery to precise and controlled trapping of nanoparticles.
Thematic Areas: Química Multidisciplinary sciences Multidisciplinary Medicine (miscellaneous) Interdisciplinar Geociências General medicine Engenharias iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vladimir.baulin@urv.cat
Author identifier: 0000-0003-2086-4271
Record's date: 2024-01-13
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.science.org/doi/10.1126/sciadv.1600261
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Science Advances. 2 (11): e1600261-
APA: Guo, Yachong; Terazzi, Emmanuel; Seemann, Ralf; Fleury, Jean Baptiste; Baulin, Vladimir A.; (2016). Direct proof of spontaneous translocation of lipid-covered hydrophobic nanoparticles through a phospholipid bilayer. Science Advances, 2(11), e1600261-. DOI: 10.1126/sciadv.1600261
Article's DOI: 10.1126/sciadv.1600261
Entity: Universitat Rovira i Virgili
Journal publication year: 2016
Publication Type: Journal Publications
Repositori URV