Articles producció científica> Química Analítica i Química Orgànica

In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case

  • Identification data

    Identifier: imarina:5131947
    Authors:
    Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus A.
    Abstract:
    Monitoring and understanding the in vitro behaviour of polyester based scaffolds both comprising the study of the hydrolytic degradation and the cell seeding viability is essential to ensure the desired functionality, according to a given biomedical purpose. As a model case to compare the performance of techniques to monitor the in vitro behaviour, poly(lactide-co-glycolide) (PLGA) scaffolds were chosen. The in vitro hydrolytic degradation of PLGA scaffolds was carried out in water and phosphate buffered saline (PBS). The evolution of the mass loss, the molar mass, the thermal properties and the surface morphology were monitored. The hydrolytic degradation media was correspondingly evaluated by means of the study of the pH, the amount of acid released and the conductivity. In addition, the in vitro biocompatibility regarding the cell culture viability was studied under physiological conditions. The cellular adhesion, cellular ability to proliferate on the scaffold, the scaffold inflammatory profile and the effect of the scaffold degradation compounds on the cells were assessed. A comparative analysis of the exploited techniques in terms of promptness of identification, depth of knowledge, simplicity of obtaining results and cost of the technique was implemented. The results showed that, depending on the balance between the interest in ascertaining the trigger of degradation or deep into the knowledge of the causes and effects of cell culture viability, an appropriate plan of analysis of the validation of polyester-based scaffolds could be designed.
  • Others:

    Author, as appears in the article.: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus A.
    Department: Química Analítica i Química Orgànica
    URV's Author/s: Serra Albet, Maria Angels
    Keywords: Water-absorption Scaffold Release Polymer testing Polymer degradation Polylactide Polyester Poly(lactide-co-glycolide) (plga) Plga Performance In vitro validation Fibrous scaffolds Erosion Degradation behavior Biocomposites Biocompatibility Bastides
    Abstract: Monitoring and understanding the in vitro behaviour of polyester based scaffolds both comprising the study of the hydrolytic degradation and the cell seeding viability is essential to ensure the desired functionality, according to a given biomedical purpose. As a model case to compare the performance of techniques to monitor the in vitro behaviour, poly(lactide-co-glycolide) (PLGA) scaffolds were chosen. The in vitro hydrolytic degradation of PLGA scaffolds was carried out in water and phosphate buffered saline (PBS). The evolution of the mass loss, the molar mass, the thermal properties and the surface morphology were monitored. The hydrolytic degradation media was correspondingly evaluated by means of the study of the pH, the amount of acid released and the conductivity. In addition, the in vitro biocompatibility regarding the cell culture viability was studied under physiological conditions. The cellular adhesion, cellular ability to proliferate on the scaffold, the scaffold inflammatory profile and the effect of the scaffold degradation compounds on the cells were assessed. A comparative analysis of the exploited techniques in terms of promptness of identification, depth of knowledge, simplicity of obtaining results and cost of the technique was implemented. The results showed that, depending on the balance between the interest in ascertaining the trigger of degradation or deep into the knowledge of the causes and effects of cell culture viability, an appropriate plan of analysis of the validation of polyester-based scaffolds could be designed.
    Thematic Areas: Química Polymers and plastics Polymer science Organic chemistry Odontología Medicina ii Medicina i Materials science, characterization & testing Materiais Interdisciplinar Engenharias iv Engenharias iii Engenharias ii Engenharias i Ciências biológicas iii Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Astronomia / física
    ISSN: 01429418
    Author's mail: angels.serra@urv.cat
    Author identifier: 0000-0003-1387-0358
    Last page: 267
    Record's date: 2024-09-07
    Journal volume: 66
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0142941817315647
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Polymer Testing. 66 256-267
    APA: Gil-Castell O., Badia J.D., Ontoria-Oviedo I., Castellano D., Marco B., Rabal A., Bou J.J., Serra A., Monreal L., Blanes M., Sepúlveda P., Ribes-Greus (2018). In vitro validation of biomedical polyester-based scaffolds: Poly(lactide-co-glycolide) as model-case. Polymer Testing, 66(), 256-267. DOI: 10.1016/j.polymertesting.2018.01.027
    Article's DOI: 10.1016/j.polymertesting.2018.01.027
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    First page: 256
    Publication Type: Journal Publications
  • Keywords:

    Materials Science, Characterization & Testing,Organic Chemistry,Polymer Science,Polymers and Plastics
    Water-absorption
    Scaffold
    Release
    Polymer testing
    Polymer degradation
    Polylactide
    Polyester
    Poly(lactide-co-glycolide) (plga)
    Plga
    Performance
    In vitro validation
    Fibrous scaffolds
    Erosion
    Degradation behavior
    Biocomposites
    Biocompatibility
    Bastides
    Química
    Polymers and plastics
    Polymer science
    Organic chemistry
    Odontología
    Medicina ii
    Medicina i
    Materials science, characterization & testing
    Materiais
    Interdisciplinar
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Engenharias i
    Ciências biológicas iii
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Astronomia / física
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