Articles producció científica> Bioquímica i Biotecnologia

Epigallocatechin-3-gallate loaded PEGylated-PLGA nanoparticles: A new anti-seizure strategy for temporal lobe epilepsy.

  • Identification data

    Identifier: imarina:5132025
    Authors:
    Cano A, Ettcheto M, Espina M, Auladell C, Calpena AC, Folch J, Barenys M, Sánchez-López E, Camins A, García ML.
    Abstract:
    Temporal lobe epilepsy is the most common type of pharmacoresistant epilepsy in adults. Epigallocatechin-3-gallate has aroused much interest because of its multiple therapeutic effects, but its instability compromises the potential effectiveness. PEGylated-PLGA nanoparticles of Epigallocatechin-3-gallate were designed to protect the drug and to increase the brain delivery. Nanoparticles were prepared by the double emulsion method and cytotoxicity, behavioral, Fluoro-Jade C, Iba1 and GFAP immunohistochemistry studies were carried out to determine their effectiveness. Nanoparticles showed an average size of 169 nm, monodisperse population, negative surface charge, encapsulation efficiency of 95% and sustained release profile. Cytotoxicity assays exhibited that these nanocarriers were non-toxic. Behavioral test showed that nanoparticles reduced most than free drug the number of epileptic episodes and their intensity. Neurotoxicity and immunohistochemistry studies confirmed a decrease in neuronal death and neuroinflammation. In conclusion, Epigallocatechin-3-gallate PEGylated-PLGA nanoparticles could be a suitable strategy for the treatment of temporal lobe epilepsy.
  • Others:

    Author, as appears in the article.: Cano A, Ettcheto M, Espina M, Auladell C, Calpena AC, Folch J, Barenys M, Sánchez-López E, Camins A, García ML.
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Folch Lopez, Jaume
    Keywords: Polymeric nanoparticles Plga-peg Neurodegeneration Epilepsy Epigallocatechin-3-gallate plga-peg epilepsy epigallocatechin-3-gallate
    Abstract: Temporal lobe epilepsy is the most common type of pharmacoresistant epilepsy in adults. Epigallocatechin-3-gallate has aroused much interest because of its multiple therapeutic effects, but its instability compromises the potential effectiveness. PEGylated-PLGA nanoparticles of Epigallocatechin-3-gallate were designed to protect the drug and to increase the brain delivery. Nanoparticles were prepared by the double emulsion method and cytotoxicity, behavioral, Fluoro-Jade C, Iba1 and GFAP immunohistochemistry studies were carried out to determine their effectiveness. Nanoparticles showed an average size of 169 nm, monodisperse population, negative surface charge, encapsulation efficiency of 95% and sustained release profile. Cytotoxicity assays exhibited that these nanocarriers were non-toxic. Behavioral test showed that nanoparticles reduced most than free drug the number of epileptic episodes and their intensity. Neurotoxicity and immunohistochemistry studies confirmed a decrease in neuronal death and neuroinflammation. In conclusion, Epigallocatechin-3-gallate PEGylated-PLGA nanoparticles could be a suitable strategy for the treatment of temporal lobe epilepsy.
    Thematic Areas: Química Psicología Pharmaceutical science Odontología Nanoscience and nanotechnology Nanoscience & nanotechnology Molecular medicine Medicine, research & experimental Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Materials science (miscellaneous) Materials science (all) Materiais Interdisciplinar General medicine General materials science Farmacia Engenharias iv Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biomedical engineering Bioengineering Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 15499634
    Author's mail: jaume.folch@urv.cat
    Author identifier: 0000-0002-5051-8858
    Record's date: 2024-09-07
    Papper version: info:eu-repo/semantics/acceptedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1549963418300315?via%3Dihub
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Nanomedicine-Nanotechnology Biology And Medicine. 14 (4): 1073-1085
    APA: Cano A, Ettcheto M, Espina M, Auladell C, Calpena AC, Folch J, Barenys M, Sánchez-López E, Camins A, García ML. (2018). Epigallocatechin-3-gallate loaded PEGylated-PLGA nanoparticles: A new anti-seizure strategy for temporal lobe epilepsy.. Nanomedicine-Nanotechnology Biology And Medicine, 14(4), 1073-1085. DOI: 10.1016/j.nano.2018.01.019
    Article's DOI: 10.1016/j.nano.2018.01.019
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    Publication Type: Journal Publications
  • Keywords:

    Bioengineering,Biomedical Engineering,Materials Science (Miscellaneous),Medicine (Miscellaneous),Medicine, Research & Experimental,Molecular Medicine,Nanoscience & Nanotechnology,Nanoscience and Nanotechnology,Pharmaceutical Science
    Polymeric nanoparticles
    Plga-peg
    Neurodegeneration
    Epilepsy
    Epigallocatechin-3-gallate
    plga-peg
    epilepsy
    epigallocatechin-3-gallate
    Química
    Psicología
    Pharmaceutical science
    Odontología
    Nanoscience and nanotechnology
    Nanoscience & nanotechnology
    Molecular medicine
    Medicine, research & experimental
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Materials science (miscellaneous)
    Materials science (all)
    Materiais
    Interdisciplinar
    General medicine
    General materials science
    Farmacia
    Engenharias iv
    Engenharias iii
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Biotecnología
    Biomedical engineering
    Bioengineering
    Astronomia / física
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