Articles producció científica> Bioquímica i Biotecnologia

Regulation of yeast fatty acid desaturase in response to iron deficiency

  • Identification data

    Identifier: imarina:5132027
    Authors:
    Romero A., Jordá T., Rozès N., Martínez-Pastor M., Puig S.
    Abstract:
    Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the Cdc48Npl4/Ufd1 complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses
  • Others:

    Author, as appears in the article.: Romero A., Jordá T., Rozès N., Martínez-Pastor M., Puig S.
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Rozès, Nicolas Andre Louis
    Keywords: Yeast Saccharomyces cerevisiae Ole1 Mga2 Iron deficiency Hypoxia Fatty acids Cold saccharomyces cerevisiae ole1 mga2 iron deficiency hypoxia fatty acids cold
    Abstract: Unsaturated fatty acids (UFA) are essential components of phospholipids that greatly contribute to the biophysical properties of cellular membranes. Biosynthesis of UFAs relies on a conserved family of iron-dependent fatty acid desaturases, whose representative in the model yeast Saccharomyces cerevisiae is Ole1. OLE1 expression is tightly regulated to adapt UFA biosynthesis and lipid bilayer properties to changes in temperature, and in UFA or oxygen availability. Despite iron deficiency being the most extended nutritional disorder worldwide, very little is known about the mechanisms and the biological relevance of fatty acid desaturases regulation in response to iron starvation. In this report, we show that endoplasmic reticulum-anchored transcription factor Mga2 activates OLE1 transcription in response to nutritional and genetic iron deficiencies. Cells lacking MGA2 display low UFA levels and do not grow under iron-limited conditions, unless UFAs are supplemented or OLE1 is overexpressed. The proteasome, E3 ubiquitin ligase Rsp5 and the Cdc48Npl4/Ufd1 complex are required for OLE1 activation during iron depletion. Interestingly, Mga2 also activates the transcription of its own mRNA in response to iron deficiency, hypoxia, low temperature and low UFAs. MGA2 up-regulation contributes to increase OLE1 expression in these situations. These results reveal the mechanism of OLE1 regulation when iron is scarce and identify the MGA2 auto-regulation as a potential activation strategy in multiple stresses
    Thematic Areas: Química Molecular biology Medicina ii Medicina i Linguística e literatura Interdisciplinar Farmacia Engenharias iii Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Cell biology Biotecnología Biophysics Biodiversidade Biochemistry & molecular biology
    ISSN: 13881981
    Author's mail: nicolasrozes@urv.cat
    Author identifier: 0000-0001-9718-3429
    Last page: 668
    Record's date: 2024-09-07
    Journal volume: 1863
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1388198118300490
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids. 1863 (6): 657-668
    APA: Romero A., Jordá T., Rozès N., Martínez-Pastor M., Puig S. (2018). Regulation of yeast fatty acid desaturase in response to iron deficiency. Biochimica Et Biophysica Acta-Molecular And Cell Biology Of Lipids, 1863(6), 657-668. DOI: 10.1016/j.bbalip.2018.03.008
    Article's DOI: 10.1016/j.bbalip.2018.03.008
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    First page: 657
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry & Molecular Biology,Biophysics,Cell Biology,Molecular Biology
    Yeast
    Saccharomyces cerevisiae
    Ole1
    Mga2
    Iron deficiency
    Hypoxia
    Fatty acids
    Cold
    saccharomyces cerevisiae
    ole1
    mga2
    iron deficiency
    hypoxia
    fatty acids
    cold
    Química
    Molecular biology
    Medicina ii
    Medicina i
    Linguística e literatura
    Interdisciplinar
    Farmacia
    Engenharias iii
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Cell biology
    Biotecnología
    Biophysics
    Biodiversidade
    Biochemistry & molecular biology
  • Documents:

  • Cerca a google

    Search to google scholar