Articles producció científica> Bioquímica i Biotecnologia

Analysis of ribosomal RNA stability in dead cells of wine yeast by quantitative PCR

  • Identification data

    Identifier: imarina:5132417
    Authors:
    Sunyer-Figueres M., Wang C., Mas A.
    Abstract:
    During wine production, some yeasts enter a Viable But Not Culturable (VBNC) state, which may influence the quality and stability of the final wine through remnant metabolic activity or by resuscitation. Culture-independent techniques are used for obtaining an accurate estimation of the number of live cells, and quantitative PCR could be the most accurate technique. As a marker of cell viability, rRNA was evaluated by analyzing its stability in dead cells. The species-specific stability of rRNA was tested in Saccharomyces cerevisiae, as well as in three species of non-Saccharomyces yeast (Hanseniaspora uvarum, Torulaspora delbrueckii and Starmerella bacillaris). High temperature and antimicrobial dimethyl dicarbonate (DMDC) treatments were efficient in lysing the yeast cells. rRNA gene and rRNA (as cDNA) were analyzed over 48 h after cell lysis by quantitative PCR. The results confirmed the stability of rRNA for 48 h after the cell lysis treatments. To sum up, rRNA may not be a good marker of cell viability in the wine yeasts that were tested.
  • Others:

    Author, as appears in the article.: Sunyer-Figueres M., Wang C., Mas A.
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Mas Baron, Alberto
    Keywords: Wine yeasts Heat Dimethyl dicarbonate (dmdc) Cell lysis heat dimethyl dicarbonate (dmdc) cell lysis
    Abstract: During wine production, some yeasts enter a Viable But Not Culturable (VBNC) state, which may influence the quality and stability of the final wine through remnant metabolic activity or by resuscitation. Culture-independent techniques are used for obtaining an accurate estimation of the number of live cells, and quantitative PCR could be the most accurate technique. As a marker of cell viability, rRNA was evaluated by analyzing its stability in dead cells. The species-specific stability of rRNA was tested in Saccharomyces cerevisiae, as well as in three species of non-Saccharomyces yeast (Hanseniaspora uvarum, Torulaspora delbrueckii and Starmerella bacillaris). High temperature and antimicrobial dimethyl dicarbonate (DMDC) treatments were efficient in lysing the yeast cells. rRNA gene and rRNA (as cDNA) were analyzed over 48 h after cell lysis by quantitative PCR. The results confirmed the stability of rRNA for 48 h after the cell lysis treatments. To sum up, rRNA may not be a good marker of cell viability in the wine yeasts that were tested.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Safety, risk, reliability and quality Química Odontología Nutrição Microbiology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Interdisciplinar Geociências General medicine Food science & technology Food science Farmacia Engenharias ii Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología Biodiversidade
    ISSN: 01681605
    Author's mail: albert.mas@urv.cat
    Author identifier: 0000-0002-0763-1679
    Last page: 4
    Record's date: 2024-09-07
    Journal volume: 270
    Papper version: info:eu-repo/semantics/submittedVersion
    Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S0168160518300369
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: International Journal Of Food Microbiology. 270 1-4
    APA: Sunyer-Figueres M., Wang C., Mas A. (2018). Analysis of ribosomal RNA stability in dead cells of wine yeast by quantitative PCR. International Journal Of Food Microbiology, 270(), 1-4. DOI: 10.1016/j.ijfoodmicro.2018.01.020
    Article's DOI: 10.1016/j.ijfoodmicro.2018.01.020
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    First page: 1
    Publication Type: Journal Publications
  • Keywords:

    Food Science,Food Science & Technology,Medicine (Miscellaneous),Microbiology,Safety, Risk, Reliability and Quality
    Wine yeasts
    Heat
    Dimethyl dicarbonate (dmdc)
    Cell lysis
    heat
    dimethyl dicarbonate (dmdc)
    cell lysis
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Safety, risk, reliability and quality
    Química
    Odontología
    Nutrição
    Microbiology
    Medicine (miscellaneous)
    Medicina veterinaria
    Medicina ii
    Medicina i
    Interdisciplinar
    Geociências
    General medicine
    Food science & technology
    Food science
    Farmacia
    Engenharias ii
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências agrárias i
    Ciência de alimentos
    Biotecnología
    Biodiversidade
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