Articles producció científica> Bioquímica i Biotecnologia

Melatonin Minimizes the Impact of Oxidative Stress Induced by Hydrogen Peroxide in Saccharomyces and Non-conventional Yeast

  • Identification data

    Identifier: imarina:5132418
    Authors:
    Vazquez, JenniferGrillitsch, KarlheinzDaum, GuentherMas, AlbertTorija, Maria-JesusBeltran, Gemma
    Abstract:
    Melatonin (N-acetyl-5-methoxytryptamine) is synthesized from tryptophan by Saccharomyces cerevisiae and non-conventional yeast species. Antioxidant properties have been suggested as a possible role of melatonin in a S. cerevisiae wine strain. However, the possible antioxidant melatonin effect on non-Saccharomyces species and other strains of S. cerevisiae must be evaluated. The aim of this study was to determine the antioxidant capacity of melatonin in eight S. cerevisiae strains and four non-conventional yeasts (Torulaspora delbrueckii, Metschnikowia pulcherrima, Starmerella bacillaris, and Hanseniaspora uvarum). Therefore, the ROS formation, lipid peroxidation, catalase activity, fatty acid composition, and peroxisome proliferation were investigated. The results showed that the presence of melatonin increases peroxisome accumulation and slightly increases the catalase activity. When cells grown in the presence of melatonin were exposed to oxidative stress induced by H2O2, lower ROS accumulation and lipid peroxidation were observed in all tested strains. Therefore, the increased catalase activity that was a consequence of oxidative stress was lower in the presence of melatonin. Moreover, the presence of MEL modulates cell FA composition, increasing oleic and palmitoleic acids and leading to higher UFA/SFA ratios, which have been previously related to a higher tolerance to H2O2. These findings demonstrate that melatonin can act as an antioxidant compound in both S. cerevisiae and non-Saccharomyces yeasts.
  • Others:

    Author, as appears in the article.: Vazquez, Jennifer; Grillitsch, Karlheinz; Daum, Guenther; Mas, Albert; Torija, Maria-Jesus; Beltran, Gemma
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / Torija Martínez, María Jesús
    Keywords: Torulaspora delbrueckii Tbars Starmerella bacillaris Ros Peroxisomes Metschnikowia pulcherrima Hanseniaspora uvarum Catalase torulaspora delbrueckii tbars starmerella bacillaris ros metschnikowia pulcherrima hanseniaspora uvarum catalase
    Abstract: Melatonin (N-acetyl-5-methoxytryptamine) is synthesized from tryptophan by Saccharomyces cerevisiae and non-conventional yeast species. Antioxidant properties have been suggested as a possible role of melatonin in a S. cerevisiae wine strain. However, the possible antioxidant melatonin effect on non-Saccharomyces species and other strains of S. cerevisiae must be evaluated. The aim of this study was to determine the antioxidant capacity of melatonin in eight S. cerevisiae strains and four non-conventional yeasts (Torulaspora delbrueckii, Metschnikowia pulcherrima, Starmerella bacillaris, and Hanseniaspora uvarum). Therefore, the ROS formation, lipid peroxidation, catalase activity, fatty acid composition, and peroxisome proliferation were investigated. The results showed that the presence of melatonin increases peroxisome accumulation and slightly increases the catalase activity. When cells grown in the presence of melatonin were exposed to oxidative stress induced by H2O2, lower ROS accumulation and lipid peroxidation were observed in all tested strains. Therefore, the increased catalase activity that was a consequence of oxidative stress was lower in the presence of melatonin. Moreover, the presence of MEL modulates cell FA composition, increasing oleic and palmitoleic acids and leading to higher UFA/SFA ratios, which have been previously related to a higher tolerance to H2O2. These findings demonstrate that melatonin can act as an antioxidant compound in both S. cerevisiae and non-Saccharomyces yeasts.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geografía Geociências Farmacia Ensino Engenharias iii Engenharias ii Engenharias i Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664302X
    Author's mail: gemma.beltran@urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
    Author identifier: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
    Record's date: 2024-10-12
    Journal volume: 9
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.frontiersin.org/articles/10.3389/fmicb.2018.01933/full
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Frontiers In Microbiology. 9 (AUG): 1933-
    APA: Vazquez, Jennifer; Grillitsch, Karlheinz; Daum, Guenther; Mas, Albert; Torija, Maria-Jesus; Beltran, Gemma (2018). Melatonin Minimizes the Impact of Oxidative Stress Induced by Hydrogen Peroxide in Saccharomyces and Non-conventional Yeast. Frontiers In Microbiology, 9(AUG), 1933-. DOI: 10.3389/fmicb.2018.01933
    Article's DOI: 10.3389/fmicb.2018.01933
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    First page: Article number 1933
    Publication Type: Journal Publications
  • Keywords:

    Microbiology,Microbiology (Medical)
    Torulaspora delbrueckii
    Tbars
    Starmerella bacillaris
    Ros
    Peroxisomes
    Metschnikowia pulcherrima
    Hanseniaspora uvarum
    Catalase
    torulaspora delbrueckii
    tbars
    starmerella bacillaris
    ros
    metschnikowia pulcherrima
    hanseniaspora uvarum
    catalase
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Microbiology (medical)
    Microbiology
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Geografía
    Geociências
    Farmacia
    Ensino
    Engenharias iii
    Engenharias ii
    Engenharias i
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
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