Functional effects of the buckwheat iminosugar d-fagomine on rats with diet-induced prediabetes.

Identifier:  imarina:5132534

Handle: https://hdl.handle.net/20.500.11797/imarina5132534

Authors:  Ramos-Romero, Sara; Hereu, Merce; Atienza, Lidia; Casas, Josefina; Taltavull, Nuria; Romeu, Marta; Amezqueta, Susana; Dasilva, Gabriel; Medina, Isabel; Torres, Josep L

Abstract:
The goals of this work are to test if d-fagomine, an iminosugar that reduces body weight gain, can delay the appearance of a fat-induced prediabetic state in a rat model and to explore possible mechanisms behind its functional action.Wistar Kyoto rats were fed a high-fat diet supplemented with d-fagomine (or not, for comparison) or a standard diet (controls) for 24 weeks. The variables measured were fasting blood glucose and insulin levels; glucose tolerance; diacylglycerols as intracellular mediators of insulin resistance in adipose tissue (AT), liver, and muscle; inflammation markers (plasma IL-6 and leptin, and liver and AT histology markers); eicosanoids from arachidonic acid as lipid mediators of inflammation; and the populations of Bacteroidetes, Firmicutes, Enterobacteriales, and Bifidobacteriales in feces. It was found that d-fagomine reduces fat-induced impaired glucose tolerance, inflammation markers, and mediators (hepatic microgranulomas and lobular inflammation, plasma IL-6, prostaglandin E2 , and leukotriene B4 ) while attenuating the changes in the populations of Enterobacteriales and Bifidobacteriales.d-Fagomine delays the development of a fat-induced prediabetic state in rats by reducing low-grade inflammation. We suggest that the anti-inflammatory effect of d-fagomine may be linked to a reduction in fat-induced overpopulation of minor gut bacteria.© 2018 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.