Author, as appears in the article.: López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J.
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Capilla Luque, Javier / Guarro Artigas, Josep / Sanchis Talón, Marta
Keywords: Whole genome sequencing Virulence factors Virulence factor Proteins Murine model infection Mucormycosis Mucor Genome comparison Gene Fungi Fungal pathogenesis Chitosan Chitin Cell-wall Aspergillus-fumigatus virulence factors virulence factor murine model infection mucormycosis mucor genome comparison fungal pathogenesis
Abstract: The increasing number of infections by species of Mucorales and their high mortality constitute an important concern for public health. This study aims to decipher the genetic basis of Mucor circinelloides pathogenicity, which displays virulence in a strain dependent manner. Assuming that genetic differences between strains may be linked to different pathotypes, we have conducted a study to explore genes responsible for virulence in M. circinelloides by whole genome sequencing of the avirulent strain NRRL3631 and comparison with the virulent strain CBS277.49. This genome analysis revealed 773 truncated, discontiguous and absent genes in the NRRL3631 strain. We also examined phenotypic traits resulting in reduced heat stress tolerance, chitosan content and lower susceptibility to toxic compounds (calcofluor white and sodium dodecyl sulphate) in the virulent strain, suggesting the influence of cell wall on pathogenesis. Based on these results, we focused on studying extracellular protein-coding genes by gene deletion and further pathotype characterization of mutants in murine models of pulmonary and systemic infection. Deletion of gene ID112092, which codes for a hypothetical extracellular protein of unknown function, resulted in significant reduction of virulence. Although pathogenesis is a multifactorial process, these findings highlight the crucial role of surface and secreted proteins in M. circinelloides virulence and should promote further studies of other differential genes.
Thematic Areas: Saúde coletiva Parasitology Odontología Microbiology (medical) Microbiology Medicina ii Medicina i Interdisciplinar Infectious diseases Immunology General medicine Farmacia Engenharias i Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 21505594
Author's mail: marta.sanchis@urv.cat javier.capilla@urv.cat
Author identifier: 0000-0001-7251-6792 0000-0002-0765-6403
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.tandfonline.com/doi/full/10.1080/21505594.2018.1435249
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Virulence. 9 (1): 707-720
APA: López-Fernández L., Sanchis M., Navarro-Rodríguez P., Nicolás F., Silva-Franco F., Guarro J., Garre V., Navarro-Mendoza M., Pérez-Arques C., Capilla J (2018). Understanding Mucor circinelloides pathogenesis by comparative genomics and phenotypical studies. Virulence, 9(1), 707-720. DOI: 10.1080/21505594.2018.1435249
Article's DOI: 10.1080/21505594.2018.1435249
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications