Author, as appears in the article.: Navarro-Mendoza MI, Pérez-Arques C, Murcia L, Martínez-García P, Lax C, Sanchis M, Capilla J, Nicolás FE, Garre V.
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Capilla Luque, Javier / Sanchis Talón, Marta
Keywords: Zygomycosis Siderophores Saccharomyces-cerevisiae Plasma-membrane Phylogenies Mucormycosis Epidemiology Antifungal Affinity iron permease Acquisition
Abstract: Mucormycosis is an emerging angio-invasive infection caused by Mucorales that presents unacceptable mortality rates. Iron uptake has been related to mucormycosis, since serum iron availability predisposes the host to suffer this infection. In addition, iron uptake has been described as a limiting factor that determines virulence in other fungal infections, becoming a promising field to study virulence in Mucorales. Here, we identified a gene family of three ferroxidases in Mucor circinelloides, fet3a, fet3b and fet3c, which are overexpressed during infection in a mouse model for mucormycosis, and their expression in vitro is regulated by the availability of iron in the culture media and the dimorphic state. Thus, only fet3a is specifically expressed during yeast growth under anaerobic conditions, whereas fet3b and fet3c are specifically expressed in mycelium during aerobic growth. A deep genetic analysis revealed partially redundant roles of the three genes, showing a predominant role of fet3c, which is required for virulence during in vivo infections, and shared functional roles with fet3b and fet3c during vegetative growth in media with low iron concentration. These results represent the first described functional specialization of an iron uptake system during fungal dimorphism.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Odontología Nutrição Multidisciplinary sciences Multidisciplinary Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Letras / linguística Interdisciplinar Geografía Geociências Farmacia Engenharias iv Engenharias iii Engenharias ii Enfermagem Educação física Educação Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 20452322
Author's mail: marta.sanchis@urv.cat javier.capilla@urv.cat
Author identifier: 0000-0001-7251-6792 0000-0002-0765-6403
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.nature.com/articles/s41598-018-26051-x
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Scientific Reports. 8 (1): 7660-
APA: Navarro-Mendoza MI, Pérez-Arques C, Murcia L, Martínez-García P, Lax C, Sanchis M, Capilla J, Nicolás FE, Garre V. (2018). Components of a new gene family of ferroxidases involved in virulence are functionally specialized in fungal dimorphism. Scientific Reports, 8(1), 7660-. DOI: 10.1038/s41598-018-26051-x
Article's DOI: 10.1038/s41598-018-26051-x
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications