Author, as appears in the article.: Karri, Venkatanaidu; Ramos, David; Bauza Martinez, Julia; Odena, Antonia; Oliveira, Eliandre; Coort, Susan L.; Evelo, Chris T.; Mariman, Edwin C. M.; Schuhmacher, Marta; Kumar, Vikas;
Department: Enginyeria Química
URV's Author/s: Kumar, Vikas / Schuhmacher Ansuategui, Marta
Keywords: Proteomics Pathway visualization Neurodegenerative diseases Metal exposure pathway visualization neurodegenerative diseases metal exposure
Abstract: Chronic exposure to heavy metals such as Pb, As, and MeHg can be associated with an increased risk of developing neurodegenerative diseases. Our in vitro bioassays results showed the potency of heavy metals in the order of Pb < As < MeHg on hippocampal cells. The main objective of this study was combining in vitro label free proteomics and systems biology approach for elucidating patterns of biological response, discovering underlying mechanisms of Pb, As, and MeHg toxicity in hippocampal cells. The omics data was refined by using different filters and normalization and multilevel analysis tools were employed to explore the data visualization. The functional and pathway visualization was performed by using Gene ontology and PathVisio tools. Using these all integrated approaches, we identified significant proteins across treatments within the mitochondrial dysfunction, oxidative stress, ubiquitin proteome dysfunction, and mRNA splicing related to neurodegenerative diseases. The systems biology analysis revealed significant alterations in proteins implicated in Parkinson's disease (PD) and Alzheimer's disease (AD). The current proteomics analysis of three metals support the insight into the proteins involved in neurodegeneration and the altered proteins can be useful for metal-specific biomarkers of exposure and its adverse effects.
Thematic Areas: Zootecnia / recursos pesqueiros Química Psicología Odontología Medicina veterinaria Medicina iii Medicina ii Medicina i Materiais Interdisciplinar Farmacia Ensino Engenharias iii Engenharias ii Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biophysics Biodiversidade Biochemistry Biochemical research methods Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: vikas.kumar@urv.cat marta.schuhmacher@urv.cat
Author identifier: 0000-0002-9795-5967 0000-0003-4381-2490
Record's date: 2024-09-07
Papper version: info:eu-repo/semantics/acceptedVersion
Link to the original source: https://www.sciencedirect.com/science/article/abs/pii/S1874391918302641
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Journal Of Proteomics. 187 106-125
APA: Karri, Venkatanaidu; Ramos, David; Bauza Martinez, Julia; Odena, Antonia; Oliveira, Eliandre; Coort, Susan L.; Evelo, Chris T.; Mariman, Edwin C. M.; (2018). Differential protein expression of hippocampal cells associated with heavy metals (Pb, As, and MeHg) neurotoxicity: Deepening into the molecular mechanism of neurodegenerative diseases. Journal Of Proteomics, 187(), 106-125. DOI: 10.1016/j.jprot.2018.06.020
Article's DOI: 10.1016/j.jprot.2018.06.020
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV