Author, as appears in the article.: Sok, Vibol; Fragoso, Alex
Department: Enginyeria Química
URV's Author/s: Fragoso Sierra, Alex / SOK, VIBOL
Keywords: Oxidation-reduction Nanostructures Horseradish peroxidase Glucose oxidase Enzymes, immobilized Enzyme stability Enzyme immobilization Cattle Carbon nano-onions Carbon Aspergillus niger Armoracia Animals Alkaline phosphatase
Abstract: Carbon nanomaterials have emerged as suitable supports for enzyme immobilization and stabilization due to their inherently large surface area, high electrical conductivity, chemical stability, and mechanical strength. In this paper, carbon nano-onions (CNOs) were used as supports to immobilize alkaline phosphatase, horseradish peroxidase, and glucose oxidase. CNOs were first functionalized by oxidation to generate carboxylic groups on the surface followed by the covalent linking of using a soluble carbodiimide as coupling agent. The CNO–enzyme conjugates were characterized by transmission electron microscopy and Raman spectroscopy. Thermogravimetric analysis revealed a specific enzyme load of ∼0.5 mg of protein per milligram of CNO. The immobilized enzymes showed enhanced storage stability without altering the optimum pH and temperatures. These properties make the prepared nanobiocatalyst of potential interest in biosensing and other biotechnological applications.
Thematic Areas: Medicine (miscellaneous) Interdisciplinar Farmacia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Biotecnología Biotechnology & applied microbiology Biotechnology Biodiversidade Biochemistry & molecular biology Biochemistry Biochemical research methods
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: alex.fragoso@urv.cat
Author identifier: 0000-0003-4839-6094
Record's date: 2025-02-08
Paper version: info:eu-repo/semantics/acceptedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Preparative Biochemistry & Biotechnology. 48 (2): 136-143
APA: Sok, Vibol; Fragoso, Alex (2018). Preparation and characterization of alkaline phosphatase, horseradish peroxidase, and glucose oxidase conjugates with carboxylated carbon nano-onions. Preparative Biochemistry & Biotechnology, 48(2), 136-143. DOI: 10.1080/10826068.2017.1405025
Entity: Universitat Rovira i Virgili
Journal publication year: 2018
Publication Type: Journal Publications
Repositori URV