Author, as appears in the article.: Mas-Capdevila A, Iglesias-Carres L, Arola-Arnal A, Aragonès G, Aleixandre A, Bravo FI, Muguerza B
Department: Bioquímica i Biotecnologia
e-ISSN: 2072-6643
URV's Author/s: Aragonès Bargalló, Gerard / Arola Arnal, Anna / Bravo Vázquez, Francisca Isabel / Muguerza Marquínez, Maria Begoña
Keywords: Spontaneously hypertensive rats Proteins Pro Oxidative stress Nitric oxide Mechanisms Endothelial dysfunction Egg-white hydrolysate Cardiovascular-diseases Blood pressure Bioactivity Bioactive peptide Antihypertensive peptides Alpha-lactorphin Ace-inhibitory peptides nitric oxide endothelial dysfunction blood pressure bioactivity bioactive peptide
Abstract: AVFQHNCQE is an antihypertensive nonapeptide obtained from a chicken foot protein hydrolysate. The present study aims to investigate the mechanisms involved in its blood pressure (BP)-lowering effect. Male (17?20 weeks old) spontaneously hypertensive rats (SHR) were used in this study. Rats were divided into two groups and orally administered water or 10 mg/kg body weight (bw) AVFQHNCQE. One hour post-administration, animals of both groups were intra-peritoneally treated with 1 mL of saline or with 1 mL of saline containing 30 mg/kg bw N?-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide (NO) synthesis, or with 1 mL of saline containing 5 mg/kg bw indomethacin, which is an inhibitor of prostacyclin synthesis (n = 6 per group). Systolic BP was recorded before oral administration and six hours after oral administration. In an additional experiment, SHR were administered water or 10 mg/kg bw AVFQHNCQE (n = 6 per group) and sacrificed six hours post-administration to study the mechanisms underlying the peptide anti-hypertensive effect. Moreover, the relaxation caused by AVFQHNCQE in isolated aortic rings from Sprague-Dawley rats was evaluated. The BP-lowering effect of the peptide was not changed after indomethacin administration but was completely abolished by L-NAME, which demonstrates that its anti-hypertensive effect is mediated by changes in endothelium-derived NO availability. In addition, AVFQHNCQE administration downregulated aortic gene expression of the vasoconstrictor factor endothelin-1 and the endothelial major free radical producer NADPH. Moreover, while no changes in plasma ACE activity were observed after its administration, liver GSH levels were higher in the peptide-treated group than in the water group, which demonstrates that AVFQHNCQE presents antioxidant properties.
Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Psicología Planejamento urbano e regional / demografia Nutrition and dietetics Nutrition & dietetics Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Food science Farmacia Engenharias iv Engenharias ii Enfermagem Educação física Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Ciência de alimentos Biotecnología
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: gerard.aragones@urv.cat franciscaisabel.bravo@urv.cat begona.muguerza@urv.cat anna.arola@urv.cat
Author identifier: 0000-0002-6468-3088 0000-0001-7384-8588 0000-0001-6529-1345
Record's date: 2023-02-18
Journal volume: 11
Papper version: info:eu-repo/semantics/publishedVersion
Papper original source: Nutrients. 11 (2): e225-
APA: Mas-Capdevila A, Iglesias-Carres L, Arola-Arnal A, Aragonès G, Aleixandre A, Bravo FI, Muguerza B (2019). Evidence that Nitric Oxide is Involved in the Blood Pressure Lowering Effect of the Peptide AVFQHNCQE in Spontaneously Hypertensive Rats.. Nutrients, 11(2), e225-. DOI: 10.3390/nu11020225
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV