Author, as appears in the article.: Pol, Laura; Eckstein, Chris; Acosta, Laura K; Xifre-Perez, Elisabet; Ferre-Borrull, Josep; Marsal, Lluis F
Department: Enginyeria Electrònica, Elèctrica i Automàtica
e-ISSN: 2079-4991
URV's Author/s: Acosta Capilla, Laura Karen / Ferré Borrull, José / Marsal Garví, Luis Francisco / POL AIRA, LAURA / Xifré Pérez, Elisabet
Keywords: Streptavidin Rifs Nanoporous anodic alumina Biotin Biosensing rifs nanoporous anodic alumina biotin biosensing
Abstract: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. The chemical modification, or functionalization, of the surfaces of nanomaterials is a key step to achieve biosensors with the best sensitivity and selectivity. The surface modification of biosensors usually comprises several modification steps that have to be optimized. Real-time monitoring of all the reactions taking place during such modification steps can be a highly helpful tool for optimization. In this work, we propose nanoporous anodic alumina (NAA) functionalized with the streptavidin-biotin complex as a platform towards label-free biosensors. Using reflective interferometric spectroscopy (RIfS), the streptavidin-biotin complex formation, using biotinylated thrombin as a molecule model, was monitored in real-time. The study compared the performance of different NAA pore sizes in order to achieve the highest response. Furthermore, the optimal streptavidin concentration that enabled the efficient detection of the biotinylated thrombin attachment was estimated. Finally, the ability of the NAA-RIfS system to quantify the concentration of biotinylated thrombin was evaluated. This study provides an optimized characterization method to monitor the chemical reactions that take place during the biotinylated molecules attachment within the NAA pores.
Thematic Areas: Physics, applied Nanoscience & nanotechnology Materials science, multidisciplinary Materials science (miscellaneous) Materials science (all) General materials science General chemical engineering Engenharias ii Chemistry, multidisciplinary Chemical engineering (miscellaneous) Chemical engineering (all)
licence for use: thttps://creativecommons.org/licenses/by/3.0/es/
ISSN: 20794991
Author's mail: laurakaren.acosta@urv.cat josep.ferre@urv.cat elisabet.xifre@urv.cat lluis.marsal@urv.cat
Author identifier: 0000-0002-5210-5133 0000-0001-6072-9889 0000-0002-5976-1408
Record's date: 2024-10-12
Journal volume: 9
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2079-4991/9/3/478
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Nanomaterials. 9 (3): 478-
APA: Pol, Laura; Eckstein, Chris; Acosta, Laura K; Xifre-Perez, Elisabet; Ferre-Borrull, Josep; Marsal, Lluis F (2019). Real-time monitoring of biotinylated molecules detection dynamics in nanoporous anodic alumina for bio-sensing. Nanomaterials, 9(3), 478-. DOI: 10.3390/nano9030478
Article's DOI: 10.3390/nano9030478
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications