Articles producció científica> Enginyeria Química

Amperometric biosensor for glyphosate based on the inhibition of tyrosinase conjugated to carbon nano-onions in a chitosan matrix on a screen-printed electrode

  • Identification data

    Identifier: imarina:5873596
    Authors:
    Sok, VibolFragoso, Alex
    Abstract:
    Glyphosate [N-(phosphonomethyl)glycine] is the most frequently used herbicide to date. Due to its indiscriminate use, it has become a globally occurring pollutant of surface waters. A biosensor for glyphosate is described here that consists of a carbon nano-onion/tyrosinase conjugate immobilized in a chitosan matrix on a screen-printed electrode. The analytical principle is based on the inhibition of the enzyme tyrosinase by glyphosate. L-DOPA is used as the enzyme substrate. The presence of the carbon nano-onions has a beneficial effect on the sensitivity of the assay. Glyphosate can be amperometrically quantified in the 0.015 to 10 μM concentration range and with a 6.5 nM (1.1 μg L-1) detection limit. The biosensor is stable more than 2 months at 4 °C. It was applied to the detection of glyphosate in water and soil samples taken from irrigation of a rice field after aerial application. Results were in good agreement with data obtained by a commercial ELISA. Graphical abstract A highly sensitive amperometric biosensor for glyphosate is reported, based on the covalent immobilization of a carbon nano-onion/tyrosinase conjugate on a chitosan matrix.
  • Others:

    Author, as appears in the article.: Sok, Vibol; Fragoso, Alex
    Department: Enginyeria Química
    URV's Author/s: Fragoso Sierra, Alex / SOK, VIBOL
    Keywords: Water pollutants, chemical Water Soil Sensors Rice Pollutant Phenol Organophosphorus Nanostructures Nanomaterial Monophenol monooxygenase Limit of detection Irrigation Immobilization Horseradish-peroxidase Herbicides Herbicide Glyphosate Glycine Enzymes, immobilized Enzyme Elisa Electrodes Electrochemical detection Contamination Chitosan Carbon Carbamate Biosensing techniques Alkaline-phosphatase
    Abstract: Glyphosate [N-(phosphonomethyl)glycine] is the most frequently used herbicide to date. Due to its indiscriminate use, it has become a globally occurring pollutant of surface waters. A biosensor for glyphosate is described here that consists of a carbon nano-onion/tyrosinase conjugate immobilized in a chitosan matrix on a screen-printed electrode. The analytical principle is based on the inhibition of the enzyme tyrosinase by glyphosate. L-DOPA is used as the enzyme substrate. The presence of the carbon nano-onions has a beneficial effect on the sensitivity of the assay. Glyphosate can be amperometrically quantified in the 0.015 to 10 μM concentration range and with a 6.5 nM (1.1 μg L-1) detection limit. The biosensor is stable more than 2 months at 4 °C. It was applied to the detection of glyphosate in water and soil samples taken from irrigation of a rice field after aerial application. Results were in good agreement with data obtained by a commercial ELISA. Graphical abstract A highly sensitive amperometric biosensor for glyphosate is reported, based on the covalent immobilization of a carbon nano-onion/tyrosinase conjugate on a chitosan matrix.
    Thematic Areas: Zootecnia / recursos pesqueiros Química Materiais Interdisciplinar Geociências Engenharias iv Engenharias ii Ciências biológicas i Ciências ambientais Chemistry, analytical Biotecnología Biodiversidade Astronomia / física Analytical chemistry
    ISSN: 00263672
    Author's mail: alex.fragoso@urv.cat
    Author identifier: 0000-0003-4839-6094
    Record's date: 2024-10-12
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Microchimica Acta. 186 (8): 569-569
    APA: Sok, Vibol; Fragoso, Alex (2019). Amperometric biosensor for glyphosate based on the inhibition of tyrosinase conjugated to carbon nano-onions in a chitosan matrix on a screen-printed electrode. Microchimica Acta, 186(8), 569-569. DOI: 10.1007/s00604-019-3672-6
    Article's DOI: 10.1007/s00604-019-3672-6
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2019
    Publication Type: Journal Publications
  • Keywords:

    Analytical Chemistry,Chemistry, Analytical
    Water pollutants, chemical
    Water
    Soil
    Sensors
    Rice
    Pollutant
    Phenol
    Organophosphorus
    Nanostructures
    Nanomaterial
    Monophenol monooxygenase
    Limit of detection
    Irrigation
    Immobilization
    Horseradish-peroxidase
    Herbicides
    Herbicide
    Glyphosate
    Glycine
    Enzymes, immobilized
    Enzyme
    Elisa
    Electrodes
    Electrochemical detection
    Contamination
    Chitosan
    Carbon
    Carbamate
    Biosensing techniques
    Alkaline-phosphatase
    Zootecnia / recursos pesqueiros
    Química
    Materiais
    Interdisciplinar
    Geociências
    Engenharias iv
    Engenharias ii
    Ciências biológicas i
    Ciências ambientais
    Chemistry, analytical
    Biotecnología
    Biodiversidade
    Astronomia / física
    Analytical chemistry
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