Articles producció científica> Ciències Mèdiques Bàsiques

Dual DNA barcoding for the molecular identification of the agents of invasive fungal infections

  • Identification data

    Identifier: imarina:5874132
    Authors:
    Hoang MTV, Irinyi L, Chen SCA, Sorrell TC,, Meyer W ISHAM Barcoding of Medical Fungi Working Group
    Abstract:
    Copyright © 2019 Hoang, Irinyi, Chen, Sorrell, the ISHAM Barcoding of Medical Fungi Working Group and Meyer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Invasive fungal infections, such as aspergillosis, candidiasis, and cryptococcosis, have significantly increased among immunocompromised people. To tackle these infections the first and most decisive step is the accurate identification of the causal pathogen. Routine identification of invasive fungal infections has progressed away from culture-dependent methods toward molecular techniques, including DNA barcoding, a highly efficient and widely used diagnostic technique. Fungal DNA barcoding previously relied on a single barcoding region, the internal transcribed spacer (ITS) region. However, this allowed only for 75% of all fungi to be correctly identified. As such, the translational elongation factor 1α (TEF1α) was recently introduced as the secondary barcode region to close the gap. Both loci together form the dual fungal DNA barcoding scheme. As a result, the ISHAM Barcoding Database has been expanded to include sequences for both barcoding regions to enable practical implementation of the dual barcoding scheme into clinical practice. The present study investigates the impact of the secondary barcode on the identification of clinically important fungal taxa, that have been demonstrated to cause severe invasive disease.
  • Others:

    Author, as appears in the article.: Hoang MTV, Irinyi L, Chen SCA, Sorrell TC,, Meyer W ISHAM Barcoding of Medical Fungi Working Group
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Guarro Artigas, Josep
    Keywords: dual barcoding system fungal dna barcoding identification internal transcribed spacer region invasive fungal diseases isham barcoding database Dual barcoding system Fungal dna barcoding Identification Internal transcribed spacer region Invasive fungal diseases Isham barcoding database Translational elongation factor 1? Translational elongation factor 1α
    Abstract: Copyright © 2019 Hoang, Irinyi, Chen, Sorrell, the ISHAM Barcoding of Medical Fungi Working Group and Meyer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. Invasive fungal infections, such as aspergillosis, candidiasis, and cryptococcosis, have significantly increased among immunocompromised people. To tackle these infections the first and most decisive step is the accurate identification of the causal pathogen. Routine identification of invasive fungal infections has progressed away from culture-dependent methods toward molecular techniques, including DNA barcoding, a highly efficient and widely used diagnostic technique. Fungal DNA barcoding previously relied on a single barcoding region, the internal transcribed spacer (ITS) region. However, this allowed only for 75% of all fungi to be correctly identified. As such, the translational elongation factor 1α (TEF1α) was recently introduced as the secondary barcode region to close the gap. Both loci together form the dual fungal DNA barcoding scheme. As a result, the ISHAM Barcoding Database has been expanded to include sequences for both barcoding regions to enable practical implementation of the dual barcoding scheme into clinical practice. The present study investigates the impact of the secondary barcode on the identification of clinically important fungal taxa, that have been demonstrated to cause severe invasive disease. Analysis of the barcoding regions was performed using barcoding gap analysis based on the genetic distances generated with the Kimura 2-parameter model. The secondary barcode demonstrated an improvement in identification for all taxa that were unidentifiable with the primary barcode, and when combined with the primary barcode ensured accurate identification for all taxa analyzed, making DNA barcoding an important, efficient and reliable addition to the diagnostic toolset of invasive fungal infections.
    Thematic Areas: Astronomia / física Biodiversidade Biotecnología Ciência da computação Ciência de alimentos Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Economia Engenharias i Engenharias ii Engenharias iii Ensino Farmacia Geociências Geografía Interdisciplinar Matemática / probabilidade e estatística Materiais Medicina i Medicina ii Medicina veterinaria Microbiology Microbiology (medical) Nutrição Odontología Química Saúde coletiva Zootecnia / recursos pesqueiros
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    Author's mail: josep.guarro@urv.cat
    ISSN: 1664302X
    Author identifier: 0000-0002-7839-7568
    Record's date: 2023-02-18
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01647/full
    Papper original source: Frontiers In Microbiology. 10 (JULY): 1647-
    APA: Hoang MTV, Irinyi L, Chen SCA, Sorrell TC,, Meyer W ISHAM Barcoding of Medical Fungi Working Group (2019). Dual DNA barcoding for the molecular identification of the agents of invasive fungal infections. Frontiers In Microbiology, 10(JULY), 1647-. DOI: 10.3389/fmicb.2019.01647
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.3389/fmicb.2019.01647
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2019
    Publication Type: Journal Publications
  • Keywords:

    Microbiology,Microbiology (Medical)
    dual barcoding system
    fungal dna barcoding
    identification
    internal transcribed spacer region
    invasive fungal diseases
    isham barcoding database
    Dual barcoding system
    Fungal dna barcoding
    Identification
    Internal transcribed spacer region
    Invasive fungal diseases
    Isham barcoding database
    Translational elongation factor 1?
    Translational elongation factor 1α
    Astronomia / física
    Biodiversidade
    Biotecnología
    Ciência da computação
    Ciência de alimentos
    Ciências agrárias i
    Ciências ambientais
    Ciências biológicas i
    Ciências biológicas ii
    Ciências biológicas iii
    Economia
    Engenharias i
    Engenharias ii
    Engenharias iii
    Ensino
    Farmacia
    Geociências
    Geografía
    Interdisciplinar
    Matemática / probabilidade e estatística
    Materiais
    Medicina i
    Medicina ii
    Medicina veterinaria
    Microbiology
    Microbiology (medical)
    Nutrição
    Odontología
    Química
    Saúde coletiva
    Zootecnia / recursos pesqueiros
    1664302X
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