Articles producció científica> Ciències Mèdiques Bàsiques

Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases Through the Supplementation with Fish Oils

  • Identification data

    Identifier: imarina:5895826
    Authors:
    Silvia Muñoz, Lucía Méndez, Gabriel Dasilva, Josep Lluís Torres, Sara Ramos-Romero, Marta Romeu, M. Rosa Nogués, Isabel Medina
    Abstract:
    The present study addressed the ability of long-chain ?-3 polyunsaturated fatty acids (?-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ?-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ?-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ?-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ?-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver.
  • Others:

    Author, as appears in the article.: Silvia Muñoz, Lucía Méndez, Gabriel Dasilva, Josep Lluís Torres, Sara Ramos-Romero, Marta Romeu, M. Rosa Nogués, Isabel Medina
    Department: Ciències Mèdiques Bàsiques
    e-ISSN: 1660-3397
    URV's Author/s: Nogués Llort, Maria Rosa / Romeu Ferran, Marta
    Keywords: Sprague-dawley rat Oxidative stress Marine omega-3 fatty acids Liver protein damage High-fat high-sucrose diet Fish oils Carbonylation sprague-dawley rat marine omega-3 fatty acids liver protein damage high-fat high-sucrose diet fish oils carbonylation
    Abstract: The present study addressed the ability of long-chain ?-3 polyunsaturated fatty acids (?-3 PUFA), i.e., eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), to ameliorate liver protein damage derived from oxidative stress and induced by consumption of high-caloric diets, typical of Westernized countries. The experimental design included an animal model of Sprague-Dawley rats fed high-fat high-sucrose (HFHS) diet supplemented with ?-3 EPA and DHA for a complete hepatic proteome analysis to map carbonylated proteins involved in specific metabolic pathways. Results showed that the intake of marine ?-3 PUFA through diet significantly decreased liver protein carbonylation caused by long-term HFHS consumption and increased antioxidant system. Fish oil modulated the carbonylation level of more than twenty liver proteins involved in critical metabolic pathways, including lipid metabolism (e.g., albumin), carbohydrate metabolism (e.g., pyruvate carboxylase), detoxification process (e.g., aldehyde dehydrogenase 2), urea cycle (e.g., carbamoyl-phosphate synthase), cytoskeleton dynamics (e.g., actin), or response to oxidative stress (e.g., catalase) among others, which might be under the control of diet marine ?-3 PUFA. In parallel, fish oil significantly changed the liver fatty acid profile given by the HFHS diet, resulting in a more anti-inflammatory phenotype. In conclusion, the present study highlights the significance of marine ?-3 PUFA intake for the health of rats fed a Westernized diet by describing several key metabolic pathways which are protected in liver.
    Thematic Areas: Zootecnia / recursos pesqueiros Química Pharmacology, toxicology and pharmaceutics (miscellaneous) Pharmacology & pharmacy Pharmaceutical science Nutrição Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Geociências Farmacia Engenharias iii Engenharias ii Educação física Drug discovery Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Chemistry, medicinal Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 16603397
    Author's mail: marta.romeu@urv.cat
    Author identifier: 0000-0002-2131-1858
    Record's date: 2024-09-07
    Journal volume: 16
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/1660-3397/16/10/353
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Marine Drugs. 16 (10): 353-360
    APA: Silvia Muñoz, Lucía Méndez, Gabriel Dasilva, Josep Lluís Torres, Sara Ramos-Romero, Marta Romeu, M. Rosa Nogués, Isabel Medina (2018). Targeting Hepatic Protein Carbonylation and Oxidative Stress Occurring on Diet-Induced Metabolic Diseases Through the Supplementation with Fish Oils. Marine Drugs, 16(10), 353-360. DOI: 10.3390/md16100353
    Article's DOI: 10.3390/md16100353
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2018
    Publication Type: Journal Publications
  • Keywords:

    Chemistry, Medicinal,Drug Discovery,Pharmaceutical Science,Pharmacology & Pharmacy,Pharmacology, Toxicology and Pharmaceutics (Miscellaneous)
    Sprague-dawley rat
    Oxidative stress
    Marine omega-3 fatty acids
    Liver protein damage
    High-fat high-sucrose diet
    Fish oils
    Carbonylation
    sprague-dawley rat
    marine omega-3 fatty acids
    liver protein damage
    high-fat high-sucrose diet
    fish oils
    carbonylation
    Zootecnia / recursos pesqueiros
    Química
    Pharmacology, toxicology and pharmaceutics (miscellaneous)
    Pharmacology & pharmacy
    Pharmaceutical science
    Nutrição
    Medicina veterinaria
    Medicina iii
    Medicina ii
    Medicina i
    Interdisciplinar
    Geociências
    Farmacia
    Engenharias iii
    Engenharias ii
    Educação física
    Drug discovery
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Chemistry, medicinal
    Biotecnología
    Biodiversidade
    Astronomia / física
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