Author, as appears in the article.: Arellano-Orden, Elena; Calero, Carmen; Lopez-Ramirez, Cecilia; Sanchez-Lopez, Veronica; Luis Lopez-Villalobos, Jose; Abad Arranz, Maria; Blanco-Orozco, Ana; Otero-Candelera, Remedios; Luis Lopez-Campos, Jose;
Department: Medicina i Cirurgia
URV's Author/s: Porras Ledantes, Jose Antonio
Keywords: Tissues Systemic inflammation Serum amyloid a Parenchyma Obstructive pulmonary-disease Inflammation Expression Copd Comorbidity Cells C-reactive protein Arterial wall parenchyma inflammation copd c-reactive protein arterial wall
Abstract: Background: Previous studies have shown that the arterial wall is a potential source of inflammatory markers in COPD. Here, we sought to compare the expression of acute phase reactants (APRs) in COPD patients and controls both at the local (pulmonary arteries and lung parenchyma) and systemic (peripheral blood leukocytes and plasma) compartments.
Methods: Consecutive patients undergoing elective surgery for suspected primary lung cancer were eligible for the study. Patients were categorized either as COPD or control group based on the spirometry results. Pulmonary arteries and lung parenchyma sections, peripheral blood leukocytes, and plasma samples were obtained from all participants. Gene expression levels of C-reactive protein (CRP) and serum amyloid A (SAA1, SAA2, and SAA4) were evaluated in tissue samples and peripheral blood leukocytes by reverse transciption-PCR. Plasma CRP and SAA protein levels were measured by enzyme-linked immunosorbent assays. Proteins were evaluated in paraffin-embedded lung tissues by immunohistochemistry.
Results: A total of 40 patients with COPD and 62 controls were enrolled. We did not find significant differences in the gene expression between COPD and control group. Both CRP and SAA were overexpressed in the lung parenchyma compared with pulmonary arteries and peripheral blood leukocytes. The expression of SAA was significantly higher in the lung parenchyma than in the pulmonary artery (2-fold higher for SAA1 and SAA4, P=0.015 and P<0.001, respectively; 8-fold higher for SAA2, P<0.001) and peripheral blood leukocytes (16-fold higher for SAA1, 439-fold higher for SAA2, and 5-fold higher for SAA4; P<0.001). No correlation between plasma levels of inflammatory markers and their expression in the lung and peripheral blood leukocytes was observed.
Conclusions: The expression of SAA in lung parenchyma is higher than in pulmonary artery and peripheral blood leukocytes. Notably, no associations were noted between lung expression of APRs and their circulating plasma levels, making the leakage of inflammatory proteins from the lung to the bloodstream unlikely. Based on these results, other potential sources of systemic inflammation in COPD (eg, the liver) need further scrutiny.
Thematic Areas: Saúde coletiva Respiratory system Pulmonary and respiratory medicine Public health, environmental and occupational health Nutrição Medicine (miscellaneous) Medicina veterinaria Medicina iii Medicina ii Medicina i Interdisciplinar Health policy Engenharias iv Enfermagem Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Biotecnología Biodiversidade
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 11782005
Author's mail: joseantonio.porras@urv.cat
Author identifier: 0000-0001-6418-1822
Record's date: 2023-02-22
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6592023/
Papper original source: International Journal Of Chronic Obstructive Pulmonary Disease. 14 1323-1332
APA: Arellano-Orden, Elena; Calero, Carmen; Lopez-Ramirez, Cecilia; Sanchez-Lopez, Veronica; Luis Lopez-Villalobos, Jose; Abad Arranz, Maria; Blanco-Orozco (2019). Evaluation of lung parenchyma, blood vessels, and peripheral blood lymphocytes as a potential source of acute phase reactants in patients with COPD. International Journal Of Chronic Obstructive Pulmonary Disease, 14(), 1323-1332. DOI: 10.2147/COPD.S188567
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.2147/COPD.S188567
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV