Articles producció científica> Bioquímica i Biotecnologia

Glycolytic Proteins Interact With Intracellular Melatonin in Saccharomyces cerevisiae

  • Identification data

    Identifier: imarina:5987740
    Authors:
    Angeles Morcillo-Parra, MariaJose Valera, MariaBeltran, GemmaMas, AlbertTorija, Maria-Jesus
    Abstract:
    Melatonin is a bioactive compound that is present in fermented beverages and synthesized by yeast during alcoholic fermentation. Many studies have shown that melatonin interacts with some mammalian proteins, such as sirtuins or orphan receptor family proteins. The aim of this study was to determine the intracellular synthesis profile of melatonin in Saccharomyces cerevisiae and to identify the proteins that may interact with this molecule in yeast cells. Melatonin from fermentation samples was analyzed by liquid chromatography mass spectrometry, and proteins bound to melatonin were immunopurified by melatonin-IgG-Dynabeads. Melatonin was produced intracellularly in the lag phase of yeast growth and was exported to the extracellular media during the stationary phase. During this period, melatonin was bound to six proteins with molecular weights from 55 to 35 kDa. Sequence analysis showed that most proteins shared high levels of homology with glycolytic enzymes. An RNA-binding protein was also identified, the elongation alpha factor, which is related to mitochondria. This study reports for the first time the interaction of melatonin and proteins inside yeast cells. These results highlight the possible role of melatonin as a signal molecule and provide a new perspective for understanding its role in yeast.
  • Others:

    Author, as appears in the article.: Angeles Morcillo-Parra, Maria; Jose Valera, Maria; Beltran, Gemma; Mas, Albert; Torija, Maria-Jesus
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Beltran Casellas, Gemma / Mas Baron, Alberto / MORCILLO PARRA, MARÍA DE LOS ÁNGELES / Torija Martínez, María Jesús / VALERA MARTÍNEZ, MARÍA JOSÉ
    Keywords: Wine yeast Receptors Pyruvate kinase Oxidative stress Mitochondria Metabolites Melatonin Isomers Glycolysis Gland hormone melatonin Gadph Fermented beverages Family Enzymes Enolase Alcoholic fermentation melatonin glycolysis gadph fermented beverages enolase
    Abstract: Melatonin is a bioactive compound that is present in fermented beverages and synthesized by yeast during alcoholic fermentation. Many studies have shown that melatonin interacts with some mammalian proteins, such as sirtuins or orphan receptor family proteins. The aim of this study was to determine the intracellular synthesis profile of melatonin in Saccharomyces cerevisiae and to identify the proteins that may interact with this molecule in yeast cells. Melatonin from fermentation samples was analyzed by liquid chromatography mass spectrometry, and proteins bound to melatonin were immunopurified by melatonin-IgG-Dynabeads. Melatonin was produced intracellularly in the lag phase of yeast growth and was exported to the extracellular media during the stationary phase. During this period, melatonin was bound to six proteins with molecular weights from 55 to 35 kDa. Sequence analysis showed that most proteins shared high levels of homology with glycolytic enzymes. An RNA-binding protein was also identified, the elongation alpha factor, which is related to mitochondria. This study reports for the first time the interaction of melatonin and proteins inside yeast cells. These results highlight the possible role of melatonin as a signal molecule and provide a new perspective for understanding its role in yeast.
    Thematic Areas: Zootecnia / recursos pesqueiros Saúde coletiva Química Odontología Nutrição Microbiology (medical) Microbiology Medicina veterinaria Medicina ii Medicina i Materiais Matemática / probabilidade e estatística Interdisciplinar Geografía Geociências Farmacia Ensino Engenharias iii Engenharias ii Engenharias i Economia Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências ambientais Ciências agrárias i Ciência de alimentos Ciência da computação Biotecnología Biodiversidade Astronomia / física
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 1664302X
    Author's mail: gemma.beltran@urv.cat mjesus.torija@urv.cat albert.mas@urv.cat
    Author identifier: 0000-0002-7071-205X 0000-0001-6419-0745 0000-0002-0763-1679
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.frontiersin.org/articles/10.3389/fmicb.2019.02424/full
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Frontiers In Microbiology. 10 (2424): 2424-
    APA: Angeles Morcillo-Parra, Maria; Jose Valera, Maria; Beltran, Gemma; Mas, Albert; Torija, Maria-Jesus (2019). Glycolytic Proteins Interact With Intracellular Melatonin in Saccharomyces cerevisiae. Frontiers In Microbiology, 10(2424), 2424-. DOI: 10.3389/fmicb.2019.02424
    Article's DOI: 10.3389/fmicb.2019.02424
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2019
    Publication Type: Journal Publications
  • Keywords:

    Microbiology,Microbiology (Medical)
    Wine yeast
    Receptors
    Pyruvate kinase
    Oxidative stress
    Mitochondria
    Metabolites
    Melatonin
    Isomers
    Glycolysis
    Gland hormone melatonin
    Gadph
    Fermented beverages
    Family
    Enzymes
    Enolase
    Alcoholic fermentation
    melatonin
    glycolysis
    gadph
    fermented beverages
    enolase
    Zootecnia / recursos pesqueiros
    Saúde coletiva
    Química
    Odontología
    Nutrição
    Microbiology (medical)
    Microbiology
    Medicina veterinaria
    Medicina ii
    Medicina i
    Materiais
    Matemática / probabilidade e estatística
    Interdisciplinar
    Geografía
    Geociências
    Farmacia
    Ensino
    Engenharias iii
    Engenharias ii
    Engenharias i
    Economia
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciências ambientais
    Ciências agrárias i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
    Biodiversidade
    Astronomia / física
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