Author, as appears in the article.: Perez-Cataluna, Aka; SaLas-Masso, Nuria; Jose Figueras, Maria;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: Figueras Salvat, María Josefa / PÉREZ CATALUÑA, ALBA
Keywords: ani arcobacter caeni arcobacter lacus isddh mlpa Algorithm Ani Arcobacter caeni Arcobacter lacus Campylobacter Genomes Helicobacter Isddh Minimal standards Mlpa Proposal Reclaimed water Taxonomy
Abstract: Two strains (RW43-9(T) and RW17-10(T)) recovered from secondary treated wastewater from the Wastewater Treatment Plant (WWTP) in Reus (Spain) were characterized by a polyphasic taxonomic study, showing evidence that they represented two novel Arcobacter species. Based on the 16S rRNA gene for strain RW43-9(T) , the closest relative was Arcobacter butzleri LMG 10828(T) (99.9 % similarity), while for strain RW17-10(T) it was Arcobacter venerupis CECT 7836(T) (99.4 %). Additionally, multilocus phylogenetic analysis of five concatenated housekeeping genes (atpA, gyrA, gyrB, hsp60 and rpoB) showed that the two strains formed separate branches that are different from known Arcobacter species. Whole genome sequences of the two strains (RW43-9(T) and RW17-10(T) ) were obtained and they were compared with those of the type strains of their nearest species. Using average nucleotide identity and in silico DNA-DNA hybridization gave values that were below 96 and 70 %, respectively. These results clearly confirm that they represent novel species. Additionally, the phenotypic characterization of the strains allowed their differentiation from other species. Therefore, the strains are proposed as representing two novel species with the names Arcobacter lacus sp. nov. (type strain RW43-9(T) =CECT 8994(T)=LMG 29062(T)) and Arcobacter caeni sp. nov. (type strain RW17-10(T) =CECT 9140(T) =LMG 29151(T)).
Thematic Areas: Biodiversidade Biotecnología Ciência de alimentos Ciências agrárias i Ciências ambientais Ciências biológicas i Ciências biológicas ii Ciências biológicas iii Ecology, evolution, behavior and systematics Engenharias ii Engenharias iii Ensino Farmacia General medicine Geociências Interdisciplinar Medicina i Medicina ii Medicina iii Medicina veterinaria Medicine (miscellaneous) Microbiology Química Saúde coletiva
licence for use: https://creativecommons.org/licenses/by/3.0/es/
Author's mail: mariajose.figueras@urv.cat
ISSN: 14665026
Author identifier: 0000-0002-2268-8980
Record's date: 2023-02-22
Papper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.003101
Papper original source: International Journal Of Systematic And Evolutionary Microbiology. 69 (11): 3326-3331
APA: Perez-Cataluna, Aka; SaLas-Masso, Nuria; Jose Figueras, Maria; (2019). Arcobacter lacus sp. nov. and Arcobacter caeni sp. nov., two novel species isolated from reclaimed water. International Journal Of Systematic And Evolutionary Microbiology, 69(11), 3326-3331. DOI: 10.1099/ijsem.0.003101
Licence document URL: http://repositori.urv.cat/ca/proteccio-de-dades/
Article's DOI: 10.1099/ijsem.0.003101
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV