Author, as appears in the article.: Iturrieta-Gonzalez, Isabel; Pujol, Isabel; Iftimie, Simona; Garcia, Dania; Morente, Vanesa; Queralt, Rosana; Guevara-Suarez, Marcela; Alastruey-Izquierdo, Ana; Ballester, Frederic; Hernandez-Restrepo, Margarita; Gene, Josepa;
Department: Ciències Mèdiques Bàsiques
URV's Author/s: García Sánchez, Dania / Gené Díaz, Josepa / Iftimie Iftimie, Simona Mihaela / Iturrieta González, Isabel Antonieta / Pujol Bajador, Isabel
Keywords: Taxonomy Sp-nov Renal-transplant recipient Posaconazole therapy Pleosporaceae Pheohyphomycosis Patient Opportunistic infections Multiple sequence alignment Molecular identification Mold Immunocompromised patients Diagnosis Barley Antifungal susceptibility Alternariosis Alternaria infectoria opportunistic infections molecular identification immunocompromised patients antifungal susceptibility alternariosis pleosporaceae alternaria infectoria
Abstract: Background Cutaneous phaeohyphomycosis is an emerging disease in immunocompromised patients, being Alternaria one of the most common genera reported as a causative agent. Species identification is not carried out mainly due to the complexity of the genus. Analysis of the ITS barcode has become standard for fungal identification, but in Alternaria it is only able to discriminate among species-groups or sections. Methods We present three cases of cutaneous infection caused by Alternaria isolates morphologically identified as belonging to section Infectoriae. They have been morphologically characterised and phylogenetically delineated with five molecular markers (ITS, ATPase, gapdh, rpb2 and tef1). Results Mycotic infections have been diagnosed by repeated cultures and histopathological examination in two of the cases. The polyphasic approach has allowed to delineate three new species of Alternaria section Infectoriae, that is A anthropophila, A atrobrunnea and A guarroi. ATPase has been the only locus able to discriminate most of the species (29 out of 31) currently sequenced in this section, including A infectoria the commonest reported species causing alternariosis. Susceptibility test showed different antifungal patterns for the three species, although terbinafine was the most active in vitro drug against these fungi. Conclusions The ATPase gene is recommended as an alternative barcode locus to identify Alternaria clinical isolates in section Infectoriae. Our results reinforce the relevance of identification of Alternaria isolates at the species level and the necessity to carry out antifungal susceptibility testing to determine the most adequate drug for treatment.
Thematic Areas: Saúde coletiva Química Odontología Mycology Medicine (miscellaneous) Medicina veterinaria Medicina ii Medicina i Materiais Interdisciplinar Infectious diseases General medicine Farmacia Engenharias iv Engenharias ii Engenharias i Enfermagem Dermatology & venereal diseases Dermatology Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciências agrárias i Biotecnología Biodiversidade Astronomia / física
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 09337407
Author's mail: simonamihaela.iftime@urv.cat isabel.pujol@urv.cat josepa.gene@urv.cat dania.garcias@urv.cat
Author identifier: 0000-0003-0714-8414 0000-0001-6195-9299 0000-0003-2397-1646
Record's date: 2024-02-03
Papper version: info:eu-repo/semantics/acceptedVersion
Papper original source: Mycoses. 63 (2): 212-224
APA: Iturrieta-Gonzalez, Isabel; Pujol, Isabel; Iftimie, Simona; Garcia, Dania; Morente, Vanesa; Queralt, Rosana; Guevara-Suarez, Marcela; Alastruey-Izquie (2020). Polyphasic identification of three new species in Alternaria section Infectoriae causing human cutaneous infection. Mycoses, 63(2), 212-224. DOI: 10.1111/myc.13026
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Entity: Universitat Rovira i Virgili
Journal publication year: 2020
Publication Type: Journal Publications
Repositori URV