Author, as appears in the article.: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M; Terra, Ximena; Ardeyol, Anna; Pinent, Montserrat; Beltran-Debon, Raul
Department: Bioquímica i Biotecnologia
URV's Author/s: Ardévol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Keywords: Up-regulation; Supplementation; Sp1; Rna, messenger; Receptor; Rats, wistar; Rats; Promoter regions, genetic; Proanthocyanidins; Polyphenols; Plant extracts; Methylation; Intestine; Intestinal mucosa; Ileum; Gut microbiota; Grape seed proanthocyanidins; Grape seed flavanols; Grape seed extract; Glucagon-like peptide-1 receptor; Glp-1r; Flavanols; Female; Epigenetics; Dna methylation; Diet-induced obesity; Cells; Animals; sp1; methylation; intestine; glp-1r; flavanols; epigenetics
Abstract: Flavonoids have been shown to modulate GLP-1 in obesity. GLP-1 induces some of its effects through the intestinal GLP-1 receptor (GLP-1R), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal GLP-1R and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated GLP-1R mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the GLP-1R promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated GLP-1R without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on GLP-1R gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.
Thematic Areas: Química; Molecular biology; Materiais; General medicine; Farmacia; Ensino; Biochemistry & molecular biology; Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2218273X
Author's mail: anna.ardevol@urv.cat; ximena.terra@urv.cat; mteresa.blay@urv.cat; montserrat.pinent@urv.cat; raul.beltran@urv.cat
Record's date: 2024-10-12
Paper version: info:eu-repo/semantics/publishedVersion
Link to the original source: https://www.mdpi.com/2218-273X/9/12/865
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Paper original source: Biomolecules. 9 (12): 865-
APA: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M; Terra, Ximena; Ardeyol, Anna; Pinent, Mont (2019). Long-Lasting Effects of GSPE on Ileal GLP-1R Gene Expression Are Associated with a Hypomethylation of the GLP-1R Promoter in Female Wistar Rats. Biomolecules, 9(12), 865-. DOI: 10.3390/biom9120865
Article's DOI: 10.3390/biom9120865
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV