Articles producció científica> Bioquímica i Biotecnologia

Long-Lasting Effects of GSPE on Ileal GLP-1R Gene Expression Are Associated with a Hypomethylation of the GLP-1R Promoter in Female Wistar Rats

  • Identification data

    Identifier: imarina:6040284
    Authors:
    Gines, IrisGil-Cardoso, KatherineD'Addario, ClaudioFalconi, AnastasiaBellia, FabioTeresa Blay, MTerra, XimenaArdeyol, AnnaPinent, MontserratBeltran-Debon, Raul
    Abstract:
    Flavonoids have been shown to modulate GLP-1 in obesity. GLP-1 induces some of its effects through the intestinal GLP-1 receptor (GLP-1R), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal GLP-1R and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated GLP-1R mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the GLP-1R promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated GLP-1R without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on GLP-1R gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.
  • Others:

    Author, as appears in the article.: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M; Terra, Ximena; Ardeyol, Anna; Pinent, Montserrat; Beltran-Debon, Raul
    Department: Bioquímica i Biotecnologia
    URV's Author/s: Ardévol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
    Keywords: Up-regulation Supplementation Sp1 Rna, messenger Receptor Rats, wistar Rats Promoter regions, genetic Proanthocyanidins Polyphenols Plant extracts Methylation Intestine Intestinal mucosa Ileum Gut microbiota Grape seed proanthocyanidins Grape seed flavanols Grape seed extract Glucagon-like peptide-1 receptor Glp-1r Flavanols Female Epigenetics Dna methylation Diet-induced obesity Cells Animals sp1 methylation intestine glp-1r flavanols epigenetics
    Abstract: Flavonoids have been shown to modulate GLP-1 in obesity. GLP-1 induces some of its effects through the intestinal GLP-1 receptor (GLP-1R), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal GLP-1R and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated GLP-1R mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the GLP-1R promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated GLP-1R without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on GLP-1R gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.
    Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 2218273X
    Author's mail: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat raul.beltran@urv.cat
    Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0001-9691-1906
    Record's date: 2024-10-12
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://www.mdpi.com/2218-273X/9/12/865
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Papper original source: Biomolecules. 9 (12): 865-
    APA: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M; Terra, Ximena; Ardeyol, Anna; Pinent, Mont (2019). Long-Lasting Effects of GSPE on Ileal GLP-1R Gene Expression Are Associated with a Hypomethylation of the GLP-1R Promoter in Female Wistar Rats. Biomolecules, 9(12), 865-. DOI: 10.3390/biom9120865
    Article's DOI: 10.3390/biom9120865
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2019
    Publication Type: Journal Publications
  • Keywords:

    Biochemistry,Biochemistry & Molecular Biology,Molecular Biology
    Up-regulation
    Supplementation
    Sp1
    Rna, messenger
    Receptor
    Rats, wistar
    Rats
    Promoter regions, genetic
    Proanthocyanidins
    Polyphenols
    Plant extracts
    Methylation
    Intestine
    Intestinal mucosa
    Ileum
    Gut microbiota
    Grape seed proanthocyanidins
    Grape seed flavanols
    Grape seed extract
    Glucagon-like peptide-1 receptor
    Glp-1r
    Flavanols
    Female
    Epigenetics
    Dna methylation
    Diet-induced obesity
    Cells
    Animals
    sp1
    methylation
    intestine
    glp-1r
    flavanols
    epigenetics
    Química
    Molecular biology
    Materiais
    General medicine
    Farmacia
    Ensino
    Biochemistry & molecular biology
    Biochemistry
  • Documents:

  • Cerca a google

    Search to google scholar