Author, as appears in the article.: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M; Terra, Ximena; Ardeyol, Anna; Pinent, Montserrat; Beltran-Debon, Raul
Department: Bioquímica i Biotecnologia
URV's Author/s: Ardévol Grau, Anna / Beltrán Debón, Raúl Alejandro / Blay Olivé, Maria Teresa / GINÉS MIR, IRIS / Pinent Armengol, Montserrat / Terra Barbadora, Ximena
Keywords: Up-regulation Supplementation Sp1 Rna, messenger Receptor Rats, wistar Rats Promoter regions, genetic Proanthocyanidins Polyphenols Plant extracts Methylation Intestine Intestinal mucosa Ileum Gut microbiota Grape seed proanthocyanidins Grape seed flavanols Grape seed extract Glucagon-like peptide-1 receptor Glp-1r Flavanols Female Epigenetics Dna methylation Diet-induced obesity Cells Animals sp1 methylation intestine glp-1r flavanols epigenetics
Abstract: Flavonoids have been shown to modulate GLP-1 in obesity. GLP-1 induces some of its effects through the intestinal GLP-1 receptor (GLP-1R), though no data exist on how flavonoids affect this receptor. Here, we examine how a dose of grape seed proanthocyanidin extract (GSPE) with anti-obesity activity affects intestinal GLP-1R and analyze whether epigenetics play a role in the long-lasting effects of GSPE. We found that 10-day GSPE administration prior to the cafeteria diet upregulated GLP-1R mRNA in the ileum 17 weeks after the GSPE treatment. This was associated with a hypomethylation of the GLP-1R promoter near the region where the SP1 transcription factor binds. In the colon, the cafeteria diet upregulated GLP-1R without showing any GSPE effect. In conclusion, we have identified long-lasting GSPE effects on GLP-1R gene expression in the ileum that are partly mediated by hypomethylation at the gene promoter and may affect the SP1 binding factor.
Thematic Areas: Química Molecular biology Materiais General medicine Farmacia Ensino Biochemistry & molecular biology Biochemistry
licence for use: https://creativecommons.org/licenses/by/3.0/es/
ISSN: 2218273X
Author's mail: anna.ardevol@urv.cat ximena.terra@urv.cat mteresa.blay@urv.cat montserrat.pinent@urv.cat raul.beltran@urv.cat
Author identifier: 0000-0003-0156-7538 0000-0003-1043-5844 0000-0002-6256-9847 0000-0003-3550-5378 0000-0001-9691-1906
Record's date: 2024-10-12
Papper version: info:eu-repo/semantics/publishedVersion
Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
Papper original source: Biomolecules. 9 (12): 865-
APA: Gines, Iris; Gil-Cardoso, Katherine; D'Addario, Claudio; Falconi, Anastasia; Bellia, Fabio; Teresa Blay, M; Terra, Ximena; Ardeyol, Anna; Pinent, Mont (2019). Long-Lasting Effects of GSPE on Ileal GLP-1R Gene Expression Are Associated with a Hypomethylation of the GLP-1R Promoter in Female Wistar Rats. Biomolecules, 9(12), 865-. DOI: 10.3390/biom9120865
Entity: Universitat Rovira i Virgili
Journal publication year: 2019
Publication Type: Journal Publications
Repositori URV