Articles producció científica> Ciències Mèdiques Bàsiques

Metabolic Rescue of Obese Adipose-Derived Stem Cells by Lin28/Let7 Pathway

  • Identification data

    Identifier: imarina:6157349
    Authors:
    Perez, Laura M.Bernal, AuroraSan Martin, NuriaLorenzo, MargaritaFernandez-Veledo, SoniaGalvez, Beatriz G.
    Abstract:
    Adipose-derived stem cells (ASCs) are promising candidates for autologous cell-based regeneration therapies by virtue of their multilineage differentiation potential and immunogenicity; however, relatively little is known about their role in adipose tissue physiology and dysfunction. Here we evaluated whether ASCs isolated from nonobese and obese tissue differed in their metabolic characteristics and differentiation potential. During differentiation to mature adipocytes, mouse and human ASCs derived from nonobese tissues both increased their insulin sensitivity and inhibition of lipolysis, whereas obese-derived ASCs were insulin-resistant, showing impaired insulin-stimulated glucose uptake and resistance to the antilipolytic effect of insulin. Furthermore, obese-derived ABCs showed enhanced release of proinflammatory cytokines and impaired production of adiponectin. Interestingly, the delivery of cytosol from control ABCs into obese-derived ASCs using a lipid-based, protein-capture methodology restored insulin sensitivity on glucose and lipid metabolism and reversed the proinflammatory cytokine profile, in part due to the restoration of Lin28 protein levels. In conclusion, glucose and lipid metabolism as well as maturation of ABCs is truncated in an obese environment. The reversal of the altered pathways in obese cells by delivery of normal subcellular fractions offers a potential new tool for cell therapy.
  • Others:

    Author, as appears in the article.: Perez, Laura M.; Bernal, Aurora; San Martin, Nuria; Lorenzo, Margarita; Fernandez-Veledo, Sonia; Galvez, Beatriz G.;
    Department: Ciències Mèdiques Bàsiques
    URV's Author/s: Fernandez Veledo, Sonia
    Keywords: Tumor-necrosis-factor Tissue Skeletal-muscle Insulin-resistance Hyperinsulinemia Glucose Free fatty-acids Differentiation Alpha Adipogenesis
    Abstract: Adipose-derived stem cells (ASCs) are promising candidates for autologous cell-based regeneration therapies by virtue of their multilineage differentiation potential and immunogenicity; however, relatively little is known about their role in adipose tissue physiology and dysfunction. Here we evaluated whether ASCs isolated from nonobese and obese tissue differed in their metabolic characteristics and differentiation potential. During differentiation to mature adipocytes, mouse and human ASCs derived from nonobese tissues both increased their insulin sensitivity and inhibition of lipolysis, whereas obese-derived ASCs were insulin-resistant, showing impaired insulin-stimulated glucose uptake and resistance to the antilipolytic effect of insulin. Furthermore, obese-derived ABCs showed enhanced release of proinflammatory cytokines and impaired production of adiponectin. Interestingly, the delivery of cytosol from control ABCs into obese-derived ASCs using a lipid-based, protein-capture methodology restored insulin sensitivity on glucose and lipid metabolism and reversed the proinflammatory cytokine profile, in part due to the restoration of Lin28 protein levels. In conclusion, glucose and lipid metabolism as well as maturation of ABCs is truncated in an obese environment. The reversal of the altered pathways in obese cells by delivery of normal subcellular fractions offers a potential new tool for cell therapy.
    Thematic Areas: Saúde coletiva Química Psicología Odontología Nutrição Medicina iii Medicina ii Medicina i Internal medicine Interdisciplinar General medicine Farmacia Engenharias iv Enfermagem Endocrinology, diabetes and metabolism Endocrinology & metabolism Educação física Ciências biológicas iii Ciências biológicas ii Ciências biológicas i Ciência de alimentos Ciência da computação Biotecnología
    licence for use: https://creativecommons.org/licenses/by/3.0/es/
    ISSN: 00121797
    Author's mail: sonia.fernandez@urv.cat
    Author identifier: 0000-0003-2906-3788
    Record's date: 2023-02-19
    Papper version: info:eu-repo/semantics/publishedVersion
    Link to the original source: https://diabetes.diabetesjournals.org/content/62/7/2368
    Papper original source: Diabetes. 62 (7): 2368-2379
    APA: Perez, Laura M.; Bernal, Aurora; San Martin, Nuria; Lorenzo, Margarita; Fernandez-Veledo, Sonia; Galvez, Beatriz G.; (2013). Metabolic Rescue of Obese Adipose-Derived Stem Cells by Lin28/Let7 Pathway. Diabetes, 62(7), 2368-2379. DOI: 10.2337/db12-1220
    Licence document URL: https://repositori.urv.cat/ca/proteccio-de-dades/
    Article's DOI: 10.2337/db12-1220
    Entity: Universitat Rovira i Virgili
    Journal publication year: 2013
    Publication Type: Journal Publications
  • Keywords:

    Endocrinology & Metabolism,Endocrinology, Diabetes and Metabolism,Internal Medicine
    Tumor-necrosis-factor
    Tissue
    Skeletal-muscle
    Insulin-resistance
    Hyperinsulinemia
    Glucose
    Free fatty-acids
    Differentiation
    Alpha
    Adipogenesis
    Saúde coletiva
    Química
    Psicología
    Odontología
    Nutrição
    Medicina iii
    Medicina ii
    Medicina i
    Internal medicine
    Interdisciplinar
    General medicine
    Farmacia
    Engenharias iv
    Enfermagem
    Endocrinology, diabetes and metabolism
    Endocrinology & metabolism
    Educação física
    Ciências biológicas iii
    Ciências biológicas ii
    Ciências biológicas i
    Ciência de alimentos
    Ciência da computação
    Biotecnología
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